RpoS proteolysis is controlled directly by ATP levels in Escherichia coli
The master regulator of stationary phase in Escherichia coli, RpoS, responds to carbon availability through changes in stability, but the individual steps in the pathway are unknown. Here we systematically block key steps of glycolysis and the citric acid cycle and monitor the effect on RpoS degrada...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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Cold Spring Harbor Laboratory Press
2014
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| Online Access: | http://hdl.handle.net/1721.1/85569 |
| _version_ | 1810990040115838976 |
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| author | Peterson, Celeste N. Levchenko, Igor Baker, Tania Rabinowitz, Joshua D. Silhavy, Thomas J. |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Peterson, Celeste N. Levchenko, Igor Baker, Tania Rabinowitz, Joshua D. Silhavy, Thomas J. |
| author_sort | Peterson, Celeste N. |
| collection | MIT |
| description | The master regulator of stationary phase in Escherichia coli, RpoS, responds to carbon availability through changes in stability, but the individual steps in the pathway are unknown. Here we systematically block key steps of glycolysis and the citric acid cycle and monitor the effect on RpoS degradation in vivo. Nutrient upshifts trigger RpoS degradation independently of protein synthesis by activating metabolic pathways that generate small energy molecules. Using metabolic mutants and inhibitors, we show that ATP, but not GTP or NADH, is necessary for RpoS degradation. In vitro reconstitution assays directly demonstrate that ClpXP fails to degrade RpoS, but not other proteins, at low ATP hydrolysis rates. These data suggest that cellular ATP levels directly control RpoS stability. |
| first_indexed | 2024-09-23T12:31:28Z |
| format | Article |
| id | mit-1721.1/85569 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T12:31:28Z |
| publishDate | 2014 |
| publisher | Cold Spring Harbor Laboratory Press |
| record_format | dspace |
| spelling | mit-1721.1/855692022-10-01T09:33:08Z RpoS proteolysis is controlled directly by ATP levels in Escherichia coli Peterson, Celeste N. Levchenko, Igor Baker, Tania Rabinowitz, Joshua D. Silhavy, Thomas J. Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. School of Science Peterson, Celeste N. Levchenko, Igor Baker, Tania The master regulator of stationary phase in Escherichia coli, RpoS, responds to carbon availability through changes in stability, but the individual steps in the pathway are unknown. Here we systematically block key steps of glycolysis and the citric acid cycle and monitor the effect on RpoS degradation in vivo. Nutrient upshifts trigger RpoS degradation independently of protein synthesis by activating metabolic pathways that generate small energy molecules. Using metabolic mutants and inhibitors, we show that ATP, but not GTP or NADH, is necessary for RpoS degradation. In vitro reconstitution assays directly demonstrate that ClpXP fails to degrade RpoS, but not other proteins, at low ATP hydrolysis rates. These data suggest that cellular ATP levels directly control RpoS stability. National Institute of General Medical Sciences (U.S.) (Grant GM049224) 2014-03-10T15:21:42Z 2014-03-10T15:21:42Z 2012-03 2011-11 Article http://purl.org/eprint/type/JournalArticle 0890-9369 1549-5477 http://hdl.handle.net/1721.1/85569 Peterson, C. N., I. Levchenko, J. D. Rabinowitz, T. A. Baker, and T. J. Silhavy. “RpoS Proteolysis Is Controlled Directly by ATP Levels in Escherichia Coli.” Genes & Development 26, no. 6 (March 15, 2012): 548–553. Copyright © 2012 by Cold Spring Harbor Laboratory Press en_US http://dx.doi.org/10.1101/gad.183517.111 Genes & Development Creative Commons Attribution‐NonCommercial License http://creativecommons.org/licenses/by-nc/4.0/ application/pdf Cold Spring Harbor Laboratory Press Genes and Development |
| spellingShingle | Peterson, Celeste N. Levchenko, Igor Baker, Tania Rabinowitz, Joshua D. Silhavy, Thomas J. RpoS proteolysis is controlled directly by ATP levels in Escherichia coli |
| title | RpoS proteolysis is controlled directly by ATP levels in Escherichia coli |
| title_full | RpoS proteolysis is controlled directly by ATP levels in Escherichia coli |
| title_fullStr | RpoS proteolysis is controlled directly by ATP levels in Escherichia coli |
| title_full_unstemmed | RpoS proteolysis is controlled directly by ATP levels in Escherichia coli |
| title_short | RpoS proteolysis is controlled directly by ATP levels in Escherichia coli |
| title_sort | rpos proteolysis is controlled directly by atp levels in escherichia coli |
| url | http://hdl.handle.net/1721.1/85569 |
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