Apical domain polarization localizes actin–myosin activity to drive ratchet-like apical constriction
Apical constriction promotes epithelia folding, which changes tissue architecture. During Drosophila gastrulation, mesoderm cells exhibit repeated contractile pulses that are stabilized such that cells apically constrict like a ratchet. The transcription factor Twist is required to stabilize cell sh...
| Main Authors: | , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Nature Publishing Group
2014
|
| Online Access: | http://hdl.handle.net/1721.1/85570 https://orcid.org/0000-0001-8060-2607 https://orcid.org/0000-0003-1338-494X |
| _version_ | 1826204909009108992 |
|---|---|
| author | Tworoger, Michael B. Martin, Adam C Mason, Frank M |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Tworoger, Michael B. Martin, Adam C Mason, Frank M |
| author_sort | Tworoger, Michael B. |
| collection | MIT |
| description | Apical constriction promotes epithelia folding, which changes tissue architecture. During Drosophila gastrulation, mesoderm cells exhibit repeated contractile pulses that are stabilized such that cells apically constrict like a ratchet. The transcription factor Twist is required to stabilize cell shape. However, it is unknown how Twist spatially coordinates downstream signals to prevent cell relaxation. We find that during constriction, Rho-associated kinase (Rok) is polarized to the middle of the apical domain (medioapical cortex), separate from adherens junctions. Rok recruits or stabilizes medioapical myosin II (Myo-II), which contracts dynamic medioapical actin cables. The formin Diaphanous mediates apical actin assembly to suppress medioapical E-cadherin localization and form stable connections between the medioapical contractile network and adherens junctions. Twist is not required for apical Rok recruitment, but instead polarizes Rok medioapically. Therefore, Twist establishes radial cell polarity of Rok/Myo-II and E-cadherin and promotes medioapical actin assembly in mesoderm cells to stabilize cell shape fluctuations. |
| first_indexed | 2024-09-23T13:03:17Z |
| format | Article |
| id | mit-1721.1/85570 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:03:17Z |
| publishDate | 2014 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/855702022-09-28T11:44:37Z Apical domain polarization localizes actin–myosin activity to drive ratchet-like apical constriction Tworoger, Michael B. Martin, Adam C Mason, Frank M Massachusetts Institute of Technology. Department of Biology Martin, Adam C. Mason, Frank M. Tworoger, Michael B. Martin, Adam C. Apical constriction promotes epithelia folding, which changes tissue architecture. During Drosophila gastrulation, mesoderm cells exhibit repeated contractile pulses that are stabilized such that cells apically constrict like a ratchet. The transcription factor Twist is required to stabilize cell shape. However, it is unknown how Twist spatially coordinates downstream signals to prevent cell relaxation. We find that during constriction, Rho-associated kinase (Rok) is polarized to the middle of the apical domain (medioapical cortex), separate from adherens junctions. Rok recruits or stabilizes medioapical myosin II (Myo-II), which contracts dynamic medioapical actin cables. The formin Diaphanous mediates apical actin assembly to suppress medioapical E-cadherin localization and form stable connections between the medioapical contractile network and adherens junctions. Twist is not required for apical Rok recruitment, but instead polarizes Rok medioapically. Therefore, Twist establishes radial cell polarity of Rok/Myo-II and E-cadherin and promotes medioapical actin assembly in mesoderm cells to stabilize cell shape fluctuations. National Institute of General Medical Sciences (U.S.) (Grant R00GM089826) 2014-03-10T15:26:48Z 2014-03-10T15:26:48Z 2013-07 2013-02 Article http://purl.org/eprint/type/JournalArticle 1465-7392 1476-4679 http://hdl.handle.net/1721.1/85570 Mason, Frank M., Michael Tworoger, and Adam C. Martin. “Apical Domain Polarization Localizes Actin–myosin Activity to Drive Ratchet-Like Apical Constriction.” Nature Cell Biology 15, no. 8 (July 7, 2013): 926–936. https://orcid.org/0000-0001-8060-2607 https://orcid.org/0000-0003-1338-494X en_US http://dx.doi.org/10.1038/ncb2796 Nature Cell Biology Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Nature Publishing Group Martin |
| spellingShingle | Tworoger, Michael B. Martin, Adam C Mason, Frank M Apical domain polarization localizes actin–myosin activity to drive ratchet-like apical constriction |
| title | Apical domain polarization localizes actin–myosin activity to drive ratchet-like apical constriction |
| title_full | Apical domain polarization localizes actin–myosin activity to drive ratchet-like apical constriction |
| title_fullStr | Apical domain polarization localizes actin–myosin activity to drive ratchet-like apical constriction |
| title_full_unstemmed | Apical domain polarization localizes actin–myosin activity to drive ratchet-like apical constriction |
| title_short | Apical domain polarization localizes actin–myosin activity to drive ratchet-like apical constriction |
| title_sort | apical domain polarization localizes actin myosin activity to drive ratchet like apical constriction |
| url | http://hdl.handle.net/1721.1/85570 https://orcid.org/0000-0001-8060-2607 https://orcid.org/0000-0003-1338-494X |
| work_keys_str_mv | AT tworogermichaelb apicaldomainpolarizationlocalizesactinmyosinactivitytodriveratchetlikeapicalconstriction AT martinadamc apicaldomainpolarizationlocalizesactinmyosinactivitytodriveratchetlikeapicalconstriction AT masonfrankm apicaldomainpolarizationlocalizesactinmyosinactivitytodriveratchetlikeapicalconstriction |