Muscle Cells Provide Instructions for Planarian Regeneration
Regeneration requires both potential and instructions for tissue replacement. In planarians, pluripotent stem cells have the potential to produce all new tissue. The identities of the cells that provide regeneration instructions are unknown. Here, we report that position control genes (PCGs) that co...
| Main Authors: | , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Elsevier B.V.
2014
|
| Online Access: | http://hdl.handle.net/1721.1/85623 https://orcid.org/0000-0002-5569-333X |
| _version_ | 1826217217312686080 |
|---|---|
| author | Witchley, Jessica N. Mayer, Mirjam Wagner, Daniel E. Owen, Jared Huntsman Reddien, Peter Witchley, Jessica N. Wagner, Daniel E. |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Witchley, Jessica N. Mayer, Mirjam Wagner, Daniel E. Owen, Jared Huntsman Reddien, Peter Witchley, Jessica N. Wagner, Daniel E. |
| author_sort | Witchley, Jessica N. |
| collection | MIT |
| description | Regeneration requires both potential and instructions for tissue replacement. In planarians, pluripotent stem cells have the potential to produce all new tissue. The identities of the cells that provide regeneration instructions are unknown. Here, we report that position control genes (PCGs) that control regeneration and tissue turnover are expressed in a subepidermal layer of nonneoblast cells. These subepidermal cells coexpress many PCGs. We propose that these subepidermal cells provide a system of body coordinates and positional information for regeneration, and identify them to be muscle cells of the planarian body wall. Almost all planarian muscle cells express PCGs, suggesting a dual function: contraction and control of patterning. PCG expression is dynamic in muscle cells after injury, even in the absence of neoblasts, suggesting that muscle is instructive for regeneration. We conclude that planarian regeneration involves two highly flexible systems: pluripotent neoblasts that can generate any new cell type and muscle cells that provide positional instructions for the regeneration of any body region. |
| first_indexed | 2024-09-23T17:00:10Z |
| format | Article |
| id | mit-1721.1/85623 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T17:00:10Z |
| publishDate | 2014 |
| publisher | Elsevier B.V. |
| record_format | dspace |
| spelling | mit-1721.1/856232022-09-29T22:59:14Z Muscle Cells Provide Instructions for Planarian Regeneration Witchley, Jessica N. Mayer, Mirjam Wagner, Daniel E. Owen, Jared Huntsman Reddien, Peter Witchley, Jessica N. Wagner, Daniel E. Massachusetts Institute of Technology. Department of Biology Whitehead Institute for Biomedical Research Owen, Jared Huntsman Witchley, Jessica N. Mayer, Mirjam Wagner, Daniel E. Reddien, Peter Regeneration requires both potential and instructions for tissue replacement. In planarians, pluripotent stem cells have the potential to produce all new tissue. The identities of the cells that provide regeneration instructions are unknown. Here, we report that position control genes (PCGs) that control regeneration and tissue turnover are expressed in a subepidermal layer of nonneoblast cells. These subepidermal cells coexpress many PCGs. We propose that these subepidermal cells provide a system of body coordinates and positional information for regeneration, and identify them to be muscle cells of the planarian body wall. Almost all planarian muscle cells express PCGs, suggesting a dual function: contraction and control of patterning. PCG expression is dynamic in muscle cells after injury, even in the absence of neoblasts, suggesting that muscle is instructive for regeneration. We conclude that planarian regeneration involves two highly flexible systems: pluripotent neoblasts that can generate any new cell type and muscle cells that provide positional instructions for the regeneration of any body region. National Institutes of Health (U.S.) (NIH R01GM080639) W. M. Keck Foundation Helen Hay Whitney Foundation (postdoctoral fellowship) 2014-03-14T15:39:28Z 2014-03-14T15:39:28Z 2013-08 2013-06 Article http://purl.org/eprint/type/JournalArticle 22111247 http://hdl.handle.net/1721.1/85623 Witchley, Jessica N., Mirjam Mayer, Daniel E. Wagner, Jared H. Owen, and Peter W. Reddien. “Muscle Cells Provide Instructions for Planarian Regeneration.” Cell Reports 4, no. 4 (August 2013): 633–641. https://orcid.org/0000-0002-5569-333X en_US http://dx.doi.org/10.1016/j.celrep.2013.07.022 Cell Reports Creative Commons Attribution http://creativecommons.org/licenses/by/3.0/ application/pdf Elsevier B.V. Elsevier |
| spellingShingle | Witchley, Jessica N. Mayer, Mirjam Wagner, Daniel E. Owen, Jared Huntsman Reddien, Peter Witchley, Jessica N. Wagner, Daniel E. Muscle Cells Provide Instructions for Planarian Regeneration |
| title | Muscle Cells Provide Instructions for Planarian Regeneration |
| title_full | Muscle Cells Provide Instructions for Planarian Regeneration |
| title_fullStr | Muscle Cells Provide Instructions for Planarian Regeneration |
| title_full_unstemmed | Muscle Cells Provide Instructions for Planarian Regeneration |
| title_short | Muscle Cells Provide Instructions for Planarian Regeneration |
| title_sort | muscle cells provide instructions for planarian regeneration |
| url | http://hdl.handle.net/1721.1/85623 https://orcid.org/0000-0002-5569-333X |
| work_keys_str_mv | AT witchleyjessican musclecellsprovideinstructionsforplanarianregeneration AT mayermirjam musclecellsprovideinstructionsforplanarianregeneration AT wagnerdaniele musclecellsprovideinstructionsforplanarianregeneration AT owenjaredhuntsman musclecellsprovideinstructionsforplanarianregeneration AT reddienpeter musclecellsprovideinstructionsforplanarianregeneration AT witchleyjessican musclecellsprovideinstructionsforplanarianregeneration AT wagnerdaniele musclecellsprovideinstructionsforplanarianregeneration |