Cactin is essential for G1 progression in Toxoplasma gondii
Toxoplasma gondii is an obligate intracellular protozoan parasite whose rapid lytic replication cycles define its pathogenicity. We identified a temperature-sensitive growth mutant, FV-P6, which irreversibly arrests before the middle of the G1 stage of the tachyzoite cell cycle. This arrest is cause...
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| Format: | Article |
| Language: | en_US |
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Wiley Blackwell
2014
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| Online Access: | http://hdl.handle.net/1721.1/85629 |
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| author | Szatanek, Tomasz Anderson-White, Brooke R. Faugno-Fusci, David M. White, Michael Gubbels, Marc-Jan Saeij, Jeroen |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Szatanek, Tomasz Anderson-White, Brooke R. Faugno-Fusci, David M. White, Michael Gubbels, Marc-Jan Saeij, Jeroen |
| author_sort | Szatanek, Tomasz |
| collection | MIT |
| description | Toxoplasma gondii is an obligate intracellular protozoan parasite whose rapid lytic replication cycles define its pathogenicity. We identified a temperature-sensitive growth mutant, FV-P6, which irreversibly arrests before the middle of the G1 stage of the tachyzoite cell cycle. This arrest is caused by a point mutation in a gene conserved across eukaryotes, Cactin, whose product localizes to the nucleus. To elucidate the role of TgCactin we performed genome-wide expression profiling. Besides the expected G1 expression profile, many genes associated with the extracellular state as well as with the bradyzoite cyst stage were identified. Consistent with these profiles were the expression of AP2 transcription factors typically associated with extracellular and bradyzoite stage parasites. This suggests a role for TgCactin in control of gene expression. As TgCactin does not contain any functionally defined domains we reasoned TgCactin exerts its function through interactions with other proteins. In support of this model we demonstrated that TgCactin is present in a protein complex and can oligomerize. Taken together, these results suggest that TgCactin acts as a pivotal protein potentially regulating gene expression at several transition points in parasite development. |
| first_indexed | 2024-09-23T10:16:34Z |
| format | Article |
| id | mit-1721.1/85629 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:16:34Z |
| publishDate | 2014 |
| publisher | Wiley Blackwell |
| record_format | dspace |
| spelling | mit-1721.1/856292022-09-26T16:54:25Z Cactin is essential for G1 progression in Toxoplasma gondii Szatanek, Tomasz Anderson-White, Brooke R. Faugno-Fusci, David M. White, Michael Gubbels, Marc-Jan Saeij, Jeroen Massachusetts Institute of Technology. Department of Biology Saeij, Jeroen Toxoplasma gondii is an obligate intracellular protozoan parasite whose rapid lytic replication cycles define its pathogenicity. We identified a temperature-sensitive growth mutant, FV-P6, which irreversibly arrests before the middle of the G1 stage of the tachyzoite cell cycle. This arrest is caused by a point mutation in a gene conserved across eukaryotes, Cactin, whose product localizes to the nucleus. To elucidate the role of TgCactin we performed genome-wide expression profiling. Besides the expected G1 expression profile, many genes associated with the extracellular state as well as with the bradyzoite cyst stage were identified. Consistent with these profiles were the expression of AP2 transcription factors typically associated with extracellular and bradyzoite stage parasites. This suggests a role for TgCactin in control of gene expression. As TgCactin does not contain any functionally defined domains we reasoned TgCactin exerts its function through interactions with other proteins. In support of this model we demonstrated that TgCactin is present in a protein complex and can oligomerize. Taken together, these results suggest that TgCactin acts as a pivotal protein potentially regulating gene expression at several transition points in parasite development. National Institutes of Health (U.S.) (Grant R01-AI080621) 2014-03-14T16:14:51Z 2014-03-14T16:14:51Z 2012-04 2012-03 Article http://purl.org/eprint/type/JournalArticle 0950382X 1365-2958 http://hdl.handle.net/1721.1/85629 Szatanek, Tomasz et al. “Cactin Is Essential for G1 Progression in Toxoplasma Gondii: Cactin Is Essential for Toxoplasma G1 Progression.” Molecular Microbiology 84.3 (2012): 566–577. en_US http://dx.doi.org/10.1111/j.1365-2958.2012.08044.x Molecular Microbiology Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Wiley Blackwell PMC |
| spellingShingle | Szatanek, Tomasz Anderson-White, Brooke R. Faugno-Fusci, David M. White, Michael Gubbels, Marc-Jan Saeij, Jeroen Cactin is essential for G1 progression in Toxoplasma gondii |
| title | Cactin is essential for G1 progression in Toxoplasma gondii |
| title_full | Cactin is essential for G1 progression in Toxoplasma gondii |
| title_fullStr | Cactin is essential for G1 progression in Toxoplasma gondii |
| title_full_unstemmed | Cactin is essential for G1 progression in Toxoplasma gondii |
| title_short | Cactin is essential for G1 progression in Toxoplasma gondii |
| title_sort | cactin is essential for g1 progression in toxoplasma gondii |
| url | http://hdl.handle.net/1721.1/85629 |
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