High-Resolution View of the Yeast Meiotic Program Revealed by Ribosome Profiling
Meiosis is a complex developmental process that generates haploid cells from diploid progenitors. We measured messenger RNA (mRNA) abundance and protein production through the yeast meiotic sporulation program and found strong, stage-specific expression for most genes, achieved through control of bo...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | en_US |
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American Association for the Advancement of Science (AAAS)
2014
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| Online Access: | http://hdl.handle.net/1721.1/85644 https://orcid.org/0000-0001-8567-2049 |
| _version_ | 1811092345933791232 |
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| author | Regev, Aviv Brar, Gloria A. Yassour, Moran Friedman, Nir Ingolia, Nicholas T. Weissman, Jonathan S. |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Regev, Aviv Brar, Gloria A. Yassour, Moran Friedman, Nir Ingolia, Nicholas T. Weissman, Jonathan S. |
| author_sort | Regev, Aviv |
| collection | MIT |
| description | Meiosis is a complex developmental process that generates haploid cells from diploid progenitors. We measured messenger RNA (mRNA) abundance and protein production through the yeast meiotic sporulation program and found strong, stage-specific expression for most genes, achieved through control of both mRNA levels and translational efficiency. Monitoring of protein production timing revealed uncharacterized recombination factors and extensive organellar remodeling. Meiotic translation is also shifted toward noncanonical sites, including short open reading frames (ORFs) on unannnotated transcripts and upstream regions of known transcripts (uORFs). Ribosome occupancy at near-cognate uORFs was associated with more efficient ORF translation; by contrast, some AUG uORFs, often exposed by regulated 5′ leader extensions, acted competitively. This work reveals pervasive translational control in meiosis and helps to illuminate the molecular basis of the broad restructuring of meiotic cells. |
| first_indexed | 2024-09-23T15:16:45Z |
| format | Article |
| id | mit-1721.1/85644 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:16:45Z |
| publishDate | 2014 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | dspace |
| spelling | mit-1721.1/856442022-09-29T13:50:59Z High-Resolution View of the Yeast Meiotic Program Revealed by Ribosome Profiling Regev, Aviv Brar, Gloria A. Yassour, Moran Friedman, Nir Ingolia, Nicholas T. Weissman, Jonathan S. Massachusetts Institute of Technology. Department of Biology Regev, Aviv Meiosis is a complex developmental process that generates haploid cells from diploid progenitors. We measured messenger RNA (mRNA) abundance and protein production through the yeast meiotic sporulation program and found strong, stage-specific expression for most genes, achieved through control of both mRNA levels and translational efficiency. Monitoring of protein production timing revealed uncharacterized recombination factors and extensive organellar remodeling. Meiotic translation is also shifted toward noncanonical sites, including short open reading frames (ORFs) on unannnotated transcripts and upstream regions of known transcripts (uORFs). Ribosome occupancy at near-cognate uORFs was associated with more efficient ORF translation; by contrast, some AUG uORFs, often exposed by regulated 5′ leader extensions, acted competitively. This work reveals pervasive translational control in meiosis and helps to illuminate the molecular basis of the broad restructuring of meiotic cells. United States-Israel Binational Science Foundation Howard Hughes Medical Institute 2014-03-14T18:15:38Z 2014-03-14T18:15:38Z 2011-12 2011-10 Article http://purl.org/eprint/type/JournalArticle 0036-8075 1095-9203 http://hdl.handle.net/1721.1/85644 Brar, G. A., M. Yassour, N. Friedman, A. Regev, N. T. Ingolia, and J. S. Weissman. “High-Resolution View of the Yeast Meiotic Program Revealed by Ribosome Profiling.” Science 335, no. 6068 (February 2, 2012): 552-557. https://orcid.org/0000-0001-8567-2049 en_US http://dx.doi.org/10.1126/science.1215110 Science Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Association for the Advancement of Science (AAAS) PMC |
| spellingShingle | Regev, Aviv Brar, Gloria A. Yassour, Moran Friedman, Nir Ingolia, Nicholas T. Weissman, Jonathan S. High-Resolution View of the Yeast Meiotic Program Revealed by Ribosome Profiling |
| title | High-Resolution View of the Yeast Meiotic Program Revealed by Ribosome Profiling |
| title_full | High-Resolution View of the Yeast Meiotic Program Revealed by Ribosome Profiling |
| title_fullStr | High-Resolution View of the Yeast Meiotic Program Revealed by Ribosome Profiling |
| title_full_unstemmed | High-Resolution View of the Yeast Meiotic Program Revealed by Ribosome Profiling |
| title_short | High-Resolution View of the Yeast Meiotic Program Revealed by Ribosome Profiling |
| title_sort | high resolution view of the yeast meiotic program revealed by ribosome profiling |
| url | http://hdl.handle.net/1721.1/85644 https://orcid.org/0000-0001-8567-2049 |
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