Polo kinase Cdc5 is a central regulator of meiosis I
During meiosis, two consecutive rounds of chromosome segregation yield four haploid gametes from one diploid cell. The Polo kinase Cdc5 is required for meiotic progression, but how Cdc5 coordinates multiple cell-cycle events during meiosis I is not understood. Here we show that CDC5-dependent phosph...
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| Format: | Article |
| Language: | en_US |
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National Academy of Sciences (U.S.)
2014
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| Online Access: | http://hdl.handle.net/1721.1/85914 https://orcid.org/0000-0003-2012-7546 https://orcid.org/0000-0001-9837-0314 |
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| author | Attner, Michelle Andrea Ee, Ly-sha Elkin, Sheryl K. Amon, Angelika B. Miller, Matthew P., Ph. D. (Matthew Paul). Massachusetts Institute of Technology |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Attner, Michelle Andrea Ee, Ly-sha Elkin, Sheryl K. Amon, Angelika B. Miller, Matthew P., Ph. D. (Matthew Paul). Massachusetts Institute of Technology |
| author_sort | Attner, Michelle Andrea |
| collection | MIT |
| description | During meiosis, two consecutive rounds of chromosome segregation yield four haploid gametes from one diploid cell. The Polo kinase Cdc5 is required for meiotic progression, but how Cdc5 coordinates multiple cell-cycle events during meiosis I is not understood. Here we show that CDC5-dependent phosphorylation of Rec8, a subunit of the cohesin complex that links sister chromatids, is required for efficient cohesin removal from chromosome arms, which is a prerequisite for meiosis I chromosome segregation. CDC5 also establishes conditions for centromeric cohesin removal during meiosis II by promoting the degradation of Spo13, a protein that protects centromeric cohesin during meiosis I. Despite CDC5’s central role in meiosis I, the protein kinase is dispensable during meiosis II and does not even phosphorylate its meiosis I targets during the second meiotic division. We conclude that Cdc5 has evolved into a master regulator of the unique meiosis I chromosome segregation pattern. |
| first_indexed | 2024-09-23T13:53:54Z |
| format | Article |
| id | mit-1721.1/85914 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:53:54Z |
| publishDate | 2014 |
| publisher | National Academy of Sciences (U.S.) |
| record_format | dspace |
| spelling | mit-1721.1/859142022-09-28T16:54:01Z Polo kinase Cdc5 is a central regulator of meiosis I Attner, Michelle Andrea Ee, Ly-sha Elkin, Sheryl K. Amon, Angelika B. Miller, Matthew P., Ph. D. (Matthew Paul). Massachusetts Institute of Technology Massachusetts Institute of Technology. Department of Biology Koch Institute for Integrative Cancer Research at MIT Attner, Michelle Andrea Miller, Matthew P. Ee, Ly-sha Elkin, Sheryl K. Amon, Angelika B. During meiosis, two consecutive rounds of chromosome segregation yield four haploid gametes from one diploid cell. The Polo kinase Cdc5 is required for meiotic progression, but how Cdc5 coordinates multiple cell-cycle events during meiosis I is not understood. Here we show that CDC5-dependent phosphorylation of Rec8, a subunit of the cohesin complex that links sister chromatids, is required for efficient cohesin removal from chromosome arms, which is a prerequisite for meiosis I chromosome segregation. CDC5 also establishes conditions for centromeric cohesin removal during meiosis II by promoting the degradation of Spo13, a protein that protects centromeric cohesin during meiosis I. Despite CDC5’s central role in meiosis I, the protein kinase is dispensable during meiosis II and does not even phosphorylate its meiosis I targets during the second meiotic division. We conclude that Cdc5 has evolved into a master regulator of the unique meiosis I chromosome segregation pattern. National Institutes of Health (U.S.) (Grant GM62207) 2014-03-24T19:09:52Z 2014-03-24T19:09:52Z 2013-08 2013-05 Article http://purl.org/eprint/type/JournalArticle 0027-8424 1091-6490 http://hdl.handle.net/1721.1/85914 Attner, M. A., M. P. Miller, L.-s. Ee, S. K. Elkin, and A. Amon. “Polo Kinase Cdc5 Is a Central Regulator of Meiosis I.” Proceedings of the National Academy of Sciences 110, no. 35 (August 27, 2013): 14278–14283. https://orcid.org/0000-0003-2012-7546 https://orcid.org/0000-0001-9837-0314 en_US http://dx.doi.org/10.1073/pnas.1311845110 Proceedings of the National Academy of Sciences Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf National Academy of Sciences (U.S.) National Academy of Science (U.S.) |
| spellingShingle | Attner, Michelle Andrea Ee, Ly-sha Elkin, Sheryl K. Amon, Angelika B. Miller, Matthew P., Ph. D. (Matthew Paul). Massachusetts Institute of Technology Polo kinase Cdc5 is a central regulator of meiosis I |
| title | Polo kinase Cdc5 is a central regulator of meiosis I |
| title_full | Polo kinase Cdc5 is a central regulator of meiosis I |
| title_fullStr | Polo kinase Cdc5 is a central regulator of meiosis I |
| title_full_unstemmed | Polo kinase Cdc5 is a central regulator of meiosis I |
| title_short | Polo kinase Cdc5 is a central regulator of meiosis I |
| title_sort | polo kinase cdc5 is a central regulator of meiosis i |
| url | http://hdl.handle.net/1721.1/85914 https://orcid.org/0000-0003-2012-7546 https://orcid.org/0000-0001-9837-0314 |
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