Photocrosslinkable Kappa-Carrageenan Hydrogels for Tissue Engineering Applications
Kappa carrageenan (κ-CA) is a natural-origin polymer that closely mimics the glycosaminoglycan structure, one of the most important constituents of native tissues extracellular matrix. Previously, it has been shown that κ-CA can crosslink via ionic interactions rendering strong, but brittle hydrogel...
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| Format: | Article |
| Language: | en_US |
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John Wiley & Sons, Inc
2014
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| Online Access: | http://hdl.handle.net/1721.1/85930 https://orcid.org/0000-0002-0284-0201 |
| _version_ | 1811094209911848960 |
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| author | Mihaila, Silvia M. Reis, Rui L. Marques, Alexandra P. Gomes, Manuela E. Khademhosseini, Ali Gaharwar, Akhilesh |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Mihaila, Silvia M. Reis, Rui L. Marques, Alexandra P. Gomes, Manuela E. Khademhosseini, Ali Gaharwar, Akhilesh |
| author_sort | Mihaila, Silvia M. |
| collection | MIT |
| description | Kappa carrageenan (κ-CA) is a natural-origin polymer that closely mimics the glycosaminoglycan structure, one of the most important constituents of native tissues extracellular matrix. Previously, it has been shown that κ-CA can crosslink via ionic interactions rendering strong, but brittle hydrogels. In this study, we introduce photocrosslinkable methacrylate moieties on the κ-CA backbone to create physically and chemically crosslinked hydrogels highlighting their use in the context of tissue engineering. By varying the degree of methacrylation, the effect on hydrogel crosslinking was investigated in terms of hydration degree, dissolution profiles, morphological, mechanical, and rheological properties. Furthermore, the viability of fibroblast cells cultured inside the photocrosslinked hydrogels was investigated. The combination of chemical and physical crosslinking procedures enables the formation of hydrogels with highly versatile physical and chemical properties, while maintaining the viability of encapsulated cells. To our best knowledge, this is the first study reporting the synthesis of photocrosslinkable κ-CA with controllable compressive moduli, swelling ratios and pore size distributions. Moreover, by micromolding approaches, spatially controlled geometries and cell distribution patterns could be obtained, thus enabling the development of cell-material platforms that can be applied and tailored to a broad range of tissue engineering strategies. |
| first_indexed | 2024-09-23T15:56:35Z |
| format | Article |
| id | mit-1721.1/85930 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:56:35Z |
| publishDate | 2014 |
| publisher | John Wiley & Sons, Inc |
| record_format | dspace |
| spelling | mit-1721.1/859302022-10-02T05:11:37Z Photocrosslinkable Kappa-Carrageenan Hydrogels for Tissue Engineering Applications Mihaila, Silvia M. Reis, Rui L. Marques, Alexandra P. Gomes, Manuela E. Khademhosseini, Ali Gaharwar, Akhilesh Harvard University--MIT Division of Health Sciences and Technology Koch Institute for Integrative Cancer Research at MIT Mihaila, Silvia M. Gaharwar, Akhilesh K. Khademhosseini, Ali Kappa carrageenan (κ-CA) is a natural-origin polymer that closely mimics the glycosaminoglycan structure, one of the most important constituents of native tissues extracellular matrix. Previously, it has been shown that κ-CA can crosslink via ionic interactions rendering strong, but brittle hydrogels. In this study, we introduce photocrosslinkable methacrylate moieties on the κ-CA backbone to create physically and chemically crosslinked hydrogels highlighting their use in the context of tissue engineering. By varying the degree of methacrylation, the effect on hydrogel crosslinking was investigated in terms of hydration degree, dissolution profiles, morphological, mechanical, and rheological properties. Furthermore, the viability of fibroblast cells cultured inside the photocrosslinked hydrogels was investigated. The combination of chemical and physical crosslinking procedures enables the formation of hydrogels with highly versatile physical and chemical properties, while maintaining the viability of encapsulated cells. To our best knowledge, this is the first study reporting the synthesis of photocrosslinkable κ-CA with controllable compressive moduli, swelling ratios and pore size distributions. Moreover, by micromolding approaches, spatially controlled geometries and cell distribution patterns could be obtained, thus enabling the development of cell-material platforms that can be applied and tailored to a broad range of tissue engineering strategies. MIT-Portugal Program Portuguese Foundation for International Cooperation in Science, Technology and Higher Education (personal grant SFRH/ BD/42968/2008) Massachusetts Institute of Technology (MIT/ECE/0047/2009 project) 2014-03-27T16:36:03Z 2014-03-27T16:36:03Z 2013-06 2012-10 Article http://purl.org/eprint/type/JournalArticle 21922640 http://hdl.handle.net/1721.1/85930 Mihaila, Silvia M., Akhilesh K. Gaharwar, Rui L. Reis, Alexandra P. Marques, Manuela E. Gomes, and Ali Khademhosseini. “ Photocrosslinkable Kappa -Carrageenan Hydrogels for Tissue Engineering Applications .” Advanced Healthcare Materials 2, no. 6 (June 2013): 895–907. https://orcid.org/0000-0002-0284-0201 en_US http://dx.doi.org/10.1002/adhm.201200317 Advanced Healthcare Materials Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf John Wiley & Sons, Inc Akhilesh Gaharwar |
| spellingShingle | Mihaila, Silvia M. Reis, Rui L. Marques, Alexandra P. Gomes, Manuela E. Khademhosseini, Ali Gaharwar, Akhilesh Photocrosslinkable Kappa-Carrageenan Hydrogels for Tissue Engineering Applications |
| title | Photocrosslinkable Kappa-Carrageenan Hydrogels for Tissue Engineering Applications |
| title_full | Photocrosslinkable Kappa-Carrageenan Hydrogels for Tissue Engineering Applications |
| title_fullStr | Photocrosslinkable Kappa-Carrageenan Hydrogels for Tissue Engineering Applications |
| title_full_unstemmed | Photocrosslinkable Kappa-Carrageenan Hydrogels for Tissue Engineering Applications |
| title_short | Photocrosslinkable Kappa-Carrageenan Hydrogels for Tissue Engineering Applications |
| title_sort | photocrosslinkable kappa carrageenan hydrogels for tissue engineering applications |
| url | http://hdl.handle.net/1721.1/85930 https://orcid.org/0000-0002-0284-0201 |
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