Fluorescent Amino Acids: Modular Building Blocks for the Assembly of New Tools for Chemical Biology
Fluorescence spectroscopy is a powerful tool for probing complex biological processes. The ubiquity of peptide–protein and protein–protein interactions in these processes has made them important targets for fluorescence labeling, and to allow sensitive readout of information concerning location, int...
| Main Authors: | , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Wiley Blackwell
2014
|
| Online Access: | http://hdl.handle.net/1721.1/85981 https://orcid.org/0000-0002-5749-7869 |
| _version_ | 1811088187258306560 |
|---|---|
| author | Krueger, Andrew T. Imperiali, Barbara |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Krueger, Andrew T. Imperiali, Barbara |
| author_sort | Krueger, Andrew T. |
| collection | MIT |
| description | Fluorescence spectroscopy is a powerful tool for probing complex biological processes. The ubiquity of peptide–protein and protein–protein interactions in these processes has made them important targets for fluorescence labeling, and to allow sensitive readout of information concerning location, interactions with other biomolecules, and macromolecular dynamics. This review describes recent advances in design, properties and applications in the area of fluorescent amino acids (FlAAs). The ability to site-selectively incorporate fluorescent amino acid building blocks into a protein or peptide of interest provides the advantage of closely retaining native function and appearance. The development of an array of fluorescent amino acids with a variety of properties, such as environment sensitivity, chelation-enhanced fluorescence, and profluorescence, has allowed researchers to gain insights into biological processes, including protein conformational changes, binding events, enzyme activities, and protein trafficking and localization. |
| first_indexed | 2024-09-23T13:57:38Z |
| format | Article |
| id | mit-1721.1/85981 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:57:38Z |
| publishDate | 2014 |
| publisher | Wiley Blackwell |
| record_format | dspace |
| spelling | mit-1721.1/859812022-10-01T18:14:35Z Fluorescent Amino Acids: Modular Building Blocks for the Assembly of New Tools for Chemical Biology Krueger, Andrew T. Imperiali, Barbara Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Chemistry Imperiali, Barbara Krueger, Andrew T. Imperiali, Barbara Fluorescence spectroscopy is a powerful tool for probing complex biological processes. The ubiquity of peptide–protein and protein–protein interactions in these processes has made them important targets for fluorescence labeling, and to allow sensitive readout of information concerning location, interactions with other biomolecules, and macromolecular dynamics. This review describes recent advances in design, properties and applications in the area of fluorescent amino acids (FlAAs). The ability to site-selectively incorporate fluorescent amino acid building blocks into a protein or peptide of interest provides the advantage of closely retaining native function and appearance. The development of an array of fluorescent amino acids with a variety of properties, such as environment sensitivity, chelation-enhanced fluorescence, and profluorescence, has allowed researchers to gain insights into biological processes, including protein conformational changes, binding events, enzyme activities, and protein trafficking and localization. 2014-04-03T13:46:07Z 2014-04-03T13:46:07Z 2013-04 2013-02 Article http://purl.org/eprint/type/JournalArticle 14394227 1439-7633 http://hdl.handle.net/1721.1/85981 Krueger, Andrew T., and Barbara Imperiali. “Fluorescent Amino Acids: Modular Building Blocks for the Assembly of New Tools for Chemical Biology.” ChemBioChem 14, no. 7 (May 10, 2013): 788–799. https://orcid.org/0000-0002-5749-7869 en_US http://dx.doi.org/10.1002/cbic.201300079 ChemBioChem Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Wiley Blackwell Imperiali via Courtney Crummett |
| spellingShingle | Krueger, Andrew T. Imperiali, Barbara Fluorescent Amino Acids: Modular Building Blocks for the Assembly of New Tools for Chemical Biology |
| title | Fluorescent Amino Acids: Modular Building Blocks for the Assembly of New Tools for Chemical Biology |
| title_full | Fluorescent Amino Acids: Modular Building Blocks for the Assembly of New Tools for Chemical Biology |
| title_fullStr | Fluorescent Amino Acids: Modular Building Blocks for the Assembly of New Tools for Chemical Biology |
| title_full_unstemmed | Fluorescent Amino Acids: Modular Building Blocks for the Assembly of New Tools for Chemical Biology |
| title_short | Fluorescent Amino Acids: Modular Building Blocks for the Assembly of New Tools for Chemical Biology |
| title_sort | fluorescent amino acids modular building blocks for the assembly of new tools for chemical biology |
| url | http://hdl.handle.net/1721.1/85981 https://orcid.org/0000-0002-5749-7869 |
| work_keys_str_mv | AT kruegerandrewt fluorescentaminoacidsmodularbuildingblocksfortheassemblyofnewtoolsforchemicalbiology AT imperialibarbara fluorescentaminoacidsmodularbuildingblocksfortheassemblyofnewtoolsforchemicalbiology |