Transcriptome Analysis of the Planarian Eye Identifies ovo as a Specific Regulator of Eye Regeneration
Among the millions of invertebrate species with visual systems, the genetic basis of eye development and function is well understood only in Drosophila melanogaster. We describe an eye transcriptome for the planarian Schmidtea mediterranea. Planarian photoreceptors expressed orthologs of genes requi...
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| Format: | Article |
| Language: | en_US |
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Elsevier B.V.
2014
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| Online Access: | http://hdl.handle.net/1721.1/86033 https://orcid.org/0000-0002-5569-333X |
| _version_ | 1826214915729260544 |
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| author | Lapan, Sylvain W. Reddien, Peter Lapan, Sylvain W. |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Lapan, Sylvain W. Reddien, Peter Lapan, Sylvain W. |
| author_sort | Lapan, Sylvain W. |
| collection | MIT |
| description | Among the millions of invertebrate species with visual systems, the genetic basis of eye development and function is well understood only in Drosophila melanogaster. We describe an eye transcriptome for the planarian Schmidtea mediterranea. Planarian photoreceptors expressed orthologs of genes required for phototransduction and microvillus structure in Drosophila and vertebrates, and optic pigment cells expressed solute transporters and melanin synthesis enzymes similar to those active in the vertebrate retinal pigment epithelium. Orthologs of several planarian eye genes, such as bestrophin-1 and Usher syndrome genes, cause eye defects in mammals when perturbed and were not previously described to have roles in invertebrate eyes. Five previously undescribed planarian eye transcription factors were required for normal eye formation during head regeneration. In particular, a conserved, transcription-factor-encoding ovo gene was expressed from the earliest stages of eye regeneration and was required for regeneration of all cell types of the eye. |
| first_indexed | 2024-09-23T16:12:17Z |
| format | Article |
| id | mit-1721.1/86033 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T16:12:17Z |
| publishDate | 2014 |
| publisher | Elsevier B.V. |
| record_format | dspace |
| spelling | mit-1721.1/860332022-10-02T07:04:49Z Transcriptome Analysis of the Planarian Eye Identifies ovo as a Specific Regulator of Eye Regeneration Lapan, Sylvain W. Reddien, Peter Lapan, Sylvain W. Massachusetts Institute of Technology. Department of Biology Whitehead Institute for Biomedical Research Lapan, Sylvain W. Reddien, Peter Among the millions of invertebrate species with visual systems, the genetic basis of eye development and function is well understood only in Drosophila melanogaster. We describe an eye transcriptome for the planarian Schmidtea mediterranea. Planarian photoreceptors expressed orthologs of genes required for phototransduction and microvillus structure in Drosophila and vertebrates, and optic pigment cells expressed solute transporters and melanin synthesis enzymes similar to those active in the vertebrate retinal pigment epithelium. Orthologs of several planarian eye genes, such as bestrophin-1 and Usher syndrome genes, cause eye defects in mammals when perturbed and were not previously described to have roles in invertebrate eyes. Five previously undescribed planarian eye transcription factors were required for normal eye formation during head regeneration. In particular, a conserved, transcription-factor-encoding ovo gene was expressed from the earliest stages of eye regeneration and was required for regeneration of all cell types of the eye. National Institutes of Health (U.S.) (NIH (R01GM08063) W. M. Keck Foundation 2014-04-04T18:38:58Z 2014-04-04T18:38:58Z 2012-08 Article http://purl.org/eprint/type/JournalArticle 22111247 http://hdl.handle.net/1721.1/86033 Lapan, Sylvain W., and Peter W. Reddien. “Transcriptome Analysis of the Planarian Eye Identifies Ovo as a Specific Regulator of Eye Regeneration.” Cell Reports 2, no. 2 (August 2012): 294–307. https://orcid.org/0000-0002-5569-333X en_US http://dx.doi.org/10.1016/j.celrep.2012.06.018 Cell Reports Creative Commons Attribution http://creativecommons.org/licenses/by/3.0/ application/pdf Elsevier B.V. Elsevier |
| spellingShingle | Lapan, Sylvain W. Reddien, Peter Lapan, Sylvain W. Transcriptome Analysis of the Planarian Eye Identifies ovo as a Specific Regulator of Eye Regeneration |
| title | Transcriptome Analysis of the Planarian Eye Identifies ovo as a Specific Regulator of Eye Regeneration |
| title_full | Transcriptome Analysis of the Planarian Eye Identifies ovo as a Specific Regulator of Eye Regeneration |
| title_fullStr | Transcriptome Analysis of the Planarian Eye Identifies ovo as a Specific Regulator of Eye Regeneration |
| title_full_unstemmed | Transcriptome Analysis of the Planarian Eye Identifies ovo as a Specific Regulator of Eye Regeneration |
| title_short | Transcriptome Analysis of the Planarian Eye Identifies ovo as a Specific Regulator of Eye Regeneration |
| title_sort | transcriptome analysis of the planarian eye identifies ovo as a specific regulator of eye regeneration |
| url | http://hdl.handle.net/1721.1/86033 https://orcid.org/0000-0002-5569-333X |
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