Cyanophycin Mediates the Accumulation and Storage of Fixed Carbon in Non-Heterocystous Filamentous Cyanobacteria from Coniform Mats
Thin, filamentous, non-heterocystous, benthic cyanobacteria (Subsection III) from some marine, lacustrine and thermal environments aggregate into macroscopic cones and conical stromatolites. We investigate the uptake and storage of inorganic carbon by cone-forming cyanobacteria from Yellowstone Nati...
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| Format: | Article |
| Language: | en_US |
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Public Library of Science
2014
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| Online Access: | http://hdl.handle.net/1721.1/86175 https://orcid.org/0000-0001-5179-5323 |
| _version_ | 1811088290360590336 |
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| author | Liang, Biqing Wu, Ting-Di Sun, Hao-Jhe Vali, Hojatollah Guerquin-Kern, Jean-Luc Wang, Chung-Ho Bosak, Tanja |
| author2 | Massachusetts Institute of Technology. Department of Earth, Atmospheric, and Planetary Sciences |
| author_facet | Massachusetts Institute of Technology. Department of Earth, Atmospheric, and Planetary Sciences Liang, Biqing Wu, Ting-Di Sun, Hao-Jhe Vali, Hojatollah Guerquin-Kern, Jean-Luc Wang, Chung-Ho Bosak, Tanja |
| author_sort | Liang, Biqing |
| collection | MIT |
| description | Thin, filamentous, non-heterocystous, benthic cyanobacteria (Subsection III) from some marine, lacustrine and thermal environments aggregate into macroscopic cones and conical stromatolites. We investigate the uptake and storage of inorganic carbon by cone-forming cyanobacteria from Yellowstone National Park using high-resolution stable isotope mapping of labeled carbon (H13CO3−) and immunoassays. Observations and incubation experiments in actively photosynthesizing enrichment cultures and field samples reveal the presence of abundant cyanophycin granules in the active growth layer of cones. These ultrastructurally heterogeneous granules rapidly accumulate newly fixed carbon and store 18% of the total particulate labeled carbon after 120 mins of incubation. The intracellular distribution of labeled carbon during the incubation experiment demonstrates an unexpectedly large contribution of PEP carboxylase to carbon fixation, and a large flow of carbon and nitrogen toward cyanophycin in thin filamentous, non-heterocystous cyanobacteria. This pattern does not occur in obvious response to a changing N or C status. Instead, it may suggest an unusual interplay between the regulation of carbon concentration mechanisms and accumulation of photorespiratory products that facilitates uptake of inorganic C and reduces photorespiration in the dense, surface-attached communities of cyanobacteria from Subsection III. |
| first_indexed | 2024-09-23T13:59:52Z |
| format | Article |
| id | mit-1721.1/86175 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:59:52Z |
| publishDate | 2014 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/861752022-09-28T17:33:53Z Cyanophycin Mediates the Accumulation and Storage of Fixed Carbon in Non-Heterocystous Filamentous Cyanobacteria from Coniform Mats Liang, Biqing Wu, Ting-Di Sun, Hao-Jhe Vali, Hojatollah Guerquin-Kern, Jean-Luc Wang, Chung-Ho Bosak, Tanja Massachusetts Institute of Technology. Department of Earth, Atmospheric, and Planetary Sciences Liang, Biqing Bosak, Tanja Thin, filamentous, non-heterocystous, benthic cyanobacteria (Subsection III) from some marine, lacustrine and thermal environments aggregate into macroscopic cones and conical stromatolites. We investigate the uptake and storage of inorganic carbon by cone-forming cyanobacteria from Yellowstone National Park using high-resolution stable isotope mapping of labeled carbon (H13CO3−) and immunoassays. Observations and incubation experiments in actively photosynthesizing enrichment cultures and field samples reveal the presence of abundant cyanophycin granules in the active growth layer of cones. These ultrastructurally heterogeneous granules rapidly accumulate newly fixed carbon and store 18% of the total particulate labeled carbon after 120 mins of incubation. The intracellular distribution of labeled carbon during the incubation experiment demonstrates an unexpectedly large contribution of PEP carboxylase to carbon fixation, and a large flow of carbon and nitrogen toward cyanophycin in thin filamentous, non-heterocystous cyanobacteria. This pattern does not occur in obvious response to a changing N or C status. Instead, it may suggest an unusual interplay between the regulation of carbon concentration mechanisms and accumulation of photorespiratory products that facilitates uptake of inorganic C and reduces photorespiration in the dense, surface-attached communities of cyanobacteria from Subsection III. Academia Sinica (Beijing, China) (postdoctoral scholar award) National Science Council (China) (NSC 102-2116-M-006-018-MY2) National Science Council (China) (NSC 101-2116-M-001-007) Massachusetts Institute of Technology (Solomon Buchsbaum Fund) 2014-04-15T19:38:18Z 2014-04-15T19:38:18Z 2014-02 Article http://purl.org/eprint/type/JournalArticle 1932-6203 http://hdl.handle.net/1721.1/86175 Liang, Biqing, Ting-Di Wu, Hao-Jhe Sun, Hojatollah Vali, Jean-Luc Guerquin-Kern, Chung-Ho Wang, and Tanja Bosak. “Cyanophycin Mediates the Accumulation and Storage of Fixed Carbon in Non-Heterocystous Filamentous Cyanobacteria from Coniform Mats.” Edited by Brett Neilan. PLoS ONE 9, no. 2 (February 7, 2014): e88142. https://orcid.org/0000-0001-5179-5323 en_US http://dx.doi.org/10.1371/journal.pone.0088142 PLoS ONE Creative Commons Attribution http://creativecommons.org/licenses/by/4.0/ application/pdf Public Library of Science PLoS |
| spellingShingle | Liang, Biqing Wu, Ting-Di Sun, Hao-Jhe Vali, Hojatollah Guerquin-Kern, Jean-Luc Wang, Chung-Ho Bosak, Tanja Cyanophycin Mediates the Accumulation and Storage of Fixed Carbon in Non-Heterocystous Filamentous Cyanobacteria from Coniform Mats |
| title | Cyanophycin Mediates the Accumulation and Storage of Fixed Carbon in Non-Heterocystous Filamentous Cyanobacteria from Coniform Mats |
| title_full | Cyanophycin Mediates the Accumulation and Storage of Fixed Carbon in Non-Heterocystous Filamentous Cyanobacteria from Coniform Mats |
| title_fullStr | Cyanophycin Mediates the Accumulation and Storage of Fixed Carbon in Non-Heterocystous Filamentous Cyanobacteria from Coniform Mats |
| title_full_unstemmed | Cyanophycin Mediates the Accumulation and Storage of Fixed Carbon in Non-Heterocystous Filamentous Cyanobacteria from Coniform Mats |
| title_short | Cyanophycin Mediates the Accumulation and Storage of Fixed Carbon in Non-Heterocystous Filamentous Cyanobacteria from Coniform Mats |
| title_sort | cyanophycin mediates the accumulation and storage of fixed carbon in non heterocystous filamentous cyanobacteria from coniform mats |
| url | http://hdl.handle.net/1721.1/86175 https://orcid.org/0000-0001-5179-5323 |
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