Functional imaging of hemodynamic stimulus response in the rat retina with ultrahigh-speed spectral / Fourier domain OCT
Measuring retinal hemodynamics in response to flicker stimulus is important for investigating pathophysiology in small animal models of diabetic retinopathy, because a reduction in the hyperemic response is thought to be one of the earliest changes in diabetic retinopathy. In this study, we investig...
| Main Authors: | , , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
SPIE
2014
|
| Online Access: | http://hdl.handle.net/1721.1/86216 https://orcid.org/0000-0002-0828-4357 |
| _version_ | 1826212143766175744 |
|---|---|
| author | Choi, Woo Jhon Baumann, Bernhard Clermont, Allen C. Feener, Edward P. Boas, David A. Fujimoto, James G. |
| author2 | Massachusetts Institute of Technology. Department of Civil and Environmental Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Civil and Environmental Engineering Choi, Woo Jhon Baumann, Bernhard Clermont, Allen C. Feener, Edward P. Boas, David A. Fujimoto, James G. |
| author_sort | Choi, Woo Jhon |
| collection | MIT |
| description | Measuring retinal hemodynamics in response to flicker stimulus is important for investigating pathophysiology in small animal models of diabetic retinopathy, because a reduction in the hyperemic response is thought to be one of the earliest changes in diabetic retinopathy. In this study, we investigated functional imaging of retinal hemodynamics in response to flicker stimulus in the rat retina using an ultrahigh speed spectral / Fourier domain OCT system at 840nm with an axial scan rate of 244kHz. At 244kHz the nominal axial velocity range that could be measured without phase wrapping was +/-37.7mm/s. Pulsatile total retinal arterial blood flow as a function of time was measured using an en face Doppler approach where a 200μm × 200μm area centered at the central retinal artery was repeatedly raster scanned at a volume acquisition rate of 55Hz. Three-dimensional capillary imaging was performed using speckle decorrelation which has minimal angle dependency compared to other angiography techniques based on OCT phase information. During OCT imaging, a flicker stimulus could be applied to the retina synchronously by inserting a dichroic mirror in the imaging interface. An acute transient increase in total retinal blood flow could be detected. At the capillary level, an increase in the degree of speckle decorrelation in capillary OCT angiography images could also be observed, which indicates an increase in the velocity of blood at the capillary level. This method promises to be useful for the investigation of small animal models of ocular diseases. © (2013) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only. |
| first_indexed | 2024-09-23T15:17:04Z |
| format | Article |
| id | mit-1721.1/86216 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:17:04Z |
| publishDate | 2014 |
| publisher | SPIE |
| record_format | dspace |
| spelling | mit-1721.1/862162022-10-02T01:55:04Z Functional imaging of hemodynamic stimulus response in the rat retina with ultrahigh-speed spectral / Fourier domain OCT Choi, Woo Jhon Baumann, Bernhard Clermont, Allen C. Feener, Edward P. Boas, David A. Fujimoto, James G. Massachusetts Institute of Technology. Department of Civil and Environmental Engineering Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Massachusetts Institute of Technology. Research Laboratory of Electronics Fujimoto, James G. Choi, Woo Jhon Baumann, Bernhard Measuring retinal hemodynamics in response to flicker stimulus is important for investigating pathophysiology in small animal models of diabetic retinopathy, because a reduction in the hyperemic response is thought to be one of the earliest changes in diabetic retinopathy. In this study, we investigated functional imaging of retinal hemodynamics in response to flicker stimulus in the rat retina using an ultrahigh speed spectral / Fourier domain OCT system at 840nm with an axial scan rate of 244kHz. At 244kHz the nominal axial velocity range that could be measured without phase wrapping was +/-37.7mm/s. Pulsatile total retinal arterial blood flow as a function of time was measured using an en face Doppler approach where a 200μm × 200μm area centered at the central retinal artery was repeatedly raster scanned at a volume acquisition rate of 55Hz. Three-dimensional capillary imaging was performed using speckle decorrelation which has minimal angle dependency compared to other angiography techniques based on OCT phase information. During OCT imaging, a flicker stimulus could be applied to the retina synchronously by inserting a dichroic mirror in the imaging interface. An acute transient increase in total retinal blood flow could be detected. At the capillary level, an increase in the degree of speckle decorrelation in capillary OCT angiography images could also be observed, which indicates an increase in the velocity of blood at the capillary level. This method promises to be useful for the investigation of small animal models of ocular diseases. © (2013) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only. United States. Air Force Office of Scientific Research (AFOSR FA9550-10-1-0551) National Institutes of Health (U.S.) (NIH R01-EY011289-26) National Institutes of Health (U.S.) (NIH R01-EY013516-09) National Institutes of Health (U.S.) (NIH R01-EY019029-04) National Institutes of Health (U.S.) (NIH R01-EY013178-12) National Institutes of Health (U.S.) (NIH R01-NS057476-05) National Institutes of Health (U.S.) (NIH R01-HL095717-04) National Institutes of Health (U.S.) (NIH R01-CA075289-15) National Institutes of Health (U.S.) (NIH R44-EY022864-01) Samsung Scholarship Foundation 2014-04-18T16:42:22Z 2014-04-18T16:42:22Z 2013-03 Article http://purl.org/eprint/type/ConferencePaper 9780819493361 0277-786X http://hdl.handle.net/1721.1/86216 Choi, WooJhon, Bernhard Baumann, Allen C. Clermont, Edward P. Feener, David A. Boas, and James G. Fujimoto. “Functional Imaging of Hemodynamic Stimulus Response in the Rat Retina with Ultrahigh-Speed Spectral / Fourier Domain OCT.” Edited by Fabrice Manns, Per G. Söderberg, and Arthur Ho. Ophthalmic Technologies XXIII (March 26, 2013). (SPIE proceedings; vol. 8567) https://orcid.org/0000-0002-0828-4357 en_US http://dx.doi.org/10.1117/12.2004096 Ophthalmic Technologies XXIII Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf SPIE SPIE |
| spellingShingle | Choi, Woo Jhon Baumann, Bernhard Clermont, Allen C. Feener, Edward P. Boas, David A. Fujimoto, James G. Functional imaging of hemodynamic stimulus response in the rat retina with ultrahigh-speed spectral / Fourier domain OCT |
| title | Functional imaging of hemodynamic stimulus response in the rat retina with ultrahigh-speed spectral / Fourier domain OCT |
| title_full | Functional imaging of hemodynamic stimulus response in the rat retina with ultrahigh-speed spectral / Fourier domain OCT |
| title_fullStr | Functional imaging of hemodynamic stimulus response in the rat retina with ultrahigh-speed spectral / Fourier domain OCT |
| title_full_unstemmed | Functional imaging of hemodynamic stimulus response in the rat retina with ultrahigh-speed spectral / Fourier domain OCT |
| title_short | Functional imaging of hemodynamic stimulus response in the rat retina with ultrahigh-speed spectral / Fourier domain OCT |
| title_sort | functional imaging of hemodynamic stimulus response in the rat retina with ultrahigh speed spectral fourier domain oct |
| url | http://hdl.handle.net/1721.1/86216 https://orcid.org/0000-0002-0828-4357 |
| work_keys_str_mv | AT choiwoojhon functionalimagingofhemodynamicstimulusresponseintheratretinawithultrahighspeedspectralfourierdomainoct AT baumannbernhard functionalimagingofhemodynamicstimulusresponseintheratretinawithultrahighspeedspectralfourierdomainoct AT clermontallenc functionalimagingofhemodynamicstimulusresponseintheratretinawithultrahighspeedspectralfourierdomainoct AT feeneredwardp functionalimagingofhemodynamicstimulusresponseintheratretinawithultrahighspeedspectralfourierdomainoct AT boasdavida functionalimagingofhemodynamicstimulusresponseintheratretinawithultrahighspeedspectralfourierdomainoct AT fujimotojamesg functionalimagingofhemodynamicstimulusresponseintheratretinawithultrahighspeedspectralfourierdomainoct |