Towards incorporation of catalytic function into small folded peptide scaffolds
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 2001.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2005
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| Online Access: | http://hdl.handle.net/1721.1/8626 |
| _version_ | 1826189625779027968 |
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| author | McDonnell, Kevin A. (Kevin Andrew), 1973- |
| author2 | Barbara Imperiali. |
| author_facet | Barbara Imperiali. McDonnell, Kevin A. (Kevin Andrew), 1973- |
| author_sort | McDonnell, Kevin A. (Kevin Andrew), 1973- |
| collection | MIT |
| description | Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 2001. |
| first_indexed | 2024-09-23T08:18:35Z |
| format | Thesis |
| id | mit-1721.1/8626 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T08:18:35Z |
| publishDate | 2005 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/86262022-01-13T07:54:21Z Towards incorporation of catalytic function into small folded peptide scaffolds McDonnell, Kevin A. (Kevin Andrew), 1973- Barbara Imperiali. Massachusetts Institute of Technology. Dept. of Chemistry. Massachusetts Institute of Technology. Department of Chemistry Chemistry. Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 2001. Includes bibliographical references. This thesis describes the development of iterative and combinatorial methods for identifying small peptide scaffolds able to support catalytic function. The incorporation of thiamine coenzyme functionality into small peptide scaffolds is achieved through the use of a coenzyme amino acid chimera (Taz). This thiazole amino acid can be alkylated to generate the active thiazolium species. A series of designed peptides containing the Taz coenzyme chimera revealed that an increasingly structured and hydrophobic peptidyl environment can dramatically enhance the acidity of the thiazolium C2 methine, the initial step in the catalytic pathway. A combinatorial approach is developed to permit a more rapid screening of large libraries of ppa peptide sequences for unique functional properties. The method used a fluorescent 13-diketone probe to identify a family of similar sequences that incorporate a functional primary amine at the center of the hydrophobic core of the PPa peptide structure. The function of the amine is enhanced by the provision of the hydrophobic peptidyl environment and is able to modestly enhance aldol condensation and retro-aldol reactions. Finally, a new trimeric Apa oligomer structure is evaluated as a functional peptide scaffold. Initially, the structural requirements of the motif are explored through a series of sequence modifications. These studies lead to the incorporation of a primary amine at a unique site in the scaffold that greatly enhances the reactivity of the amine. It is further demonstrated that this increased functional ability is directly related to the folded stability of the trimer. by Kevin A. McDonnell. Ph.D. 2005-08-23T21:49:34Z 2005-08-23T21:49:34Z 2001 2001 Thesis http://hdl.handle.net/1721.1/8626 49544015 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 305 leaves 20975453 bytes 20975210 bytes application/pdf application/pdf application/pdf Massachusetts Institute of Technology |
| spellingShingle | Chemistry. McDonnell, Kevin A. (Kevin Andrew), 1973- Towards incorporation of catalytic function into small folded peptide scaffolds |
| title | Towards incorporation of catalytic function into small folded peptide scaffolds |
| title_full | Towards incorporation of catalytic function into small folded peptide scaffolds |
| title_fullStr | Towards incorporation of catalytic function into small folded peptide scaffolds |
| title_full_unstemmed | Towards incorporation of catalytic function into small folded peptide scaffolds |
| title_short | Towards incorporation of catalytic function into small folded peptide scaffolds |
| title_sort | towards incorporation of catalytic function into small folded peptide scaffolds |
| topic | Chemistry. |
| url | http://hdl.handle.net/1721.1/8626 |
| work_keys_str_mv | AT mcdonnellkevinakevinandrew1973 towardsincorporationofcatalyticfunctionintosmallfoldedpeptidescaffolds |