Isolation and retrieval of circulating tumor cells using centrifugal forces
Presence and frequency of rare circulating tumor cells (CTCs) in bloodstreams of cancer patients are pivotal to early cancer detection and treatment monitoring. Here, we use a spiral microchannel with inherent centrifugal forces for continuous, size-based separation of CTCs from blood (Dean Flow Fra...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2014
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| Online Access: | http://hdl.handle.net/1721.1/86978 https://orcid.org/0000-0001-7215-1439 |
| _version_ | 1826198122644111360 |
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| author | Hou, Han Wei Warkiani, Majid Ebrahimi Khoo, Bee Luan Li, Zi Rui Soo, Ross A. Tan, Daniel Shao-Weng Lim, Wan-Teck Han, Jongyoon Bhagat, Ali Asgar S. Lim, Chwee Teck |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Hou, Han Wei Warkiani, Majid Ebrahimi Khoo, Bee Luan Li, Zi Rui Soo, Ross A. Tan, Daniel Shao-Weng Lim, Wan-Teck Han, Jongyoon Bhagat, Ali Asgar S. Lim, Chwee Teck |
| author_sort | Hou, Han Wei |
| collection | MIT |
| description | Presence and frequency of rare circulating tumor cells (CTCs) in bloodstreams of cancer patients are pivotal to early cancer detection and treatment monitoring. Here, we use a spiral microchannel with inherent centrifugal forces for continuous, size-based separation of CTCs from blood (Dean Flow Fractionation (DFF)) which facilitates easy coupling with conventional downstream biological assays. Device performance was optimized using cancer cell lines (> 85% recovery), followed by clinical validation with positive CTCs enumeration in all samples from patients with metastatic lung cancer (n = 20; 5–88 CTCs per mL). The presence of CD133+ cells, a phenotypic marker characteristic of stem-like behavior in lung cancer cells was also identified in the isolated subpopulation of CTCs. The spiral biochip identifies and addresses key challenges of the next generation CTCs isolation assay including antibody independent isolation, high sensitivity and throughput (3 mL/hr); and single-step retrieval of viable CTCs. |
| first_indexed | 2024-09-23T10:59:19Z |
| format | Article |
| id | mit-1721.1/86978 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:59:19Z |
| publishDate | 2014 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/869782022-10-01T00:23:50Z Isolation and retrieval of circulating tumor cells using centrifugal forces Hou, Han Wei Warkiani, Majid Ebrahimi Khoo, Bee Luan Li, Zi Rui Soo, Ross A. Tan, Daniel Shao-Weng Lim, Wan-Teck Han, Jongyoon Bhagat, Ali Asgar S. Lim, Chwee Teck Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Han, Jongyoon Hou, Han Wei Presence and frequency of rare circulating tumor cells (CTCs) in bloodstreams of cancer patients are pivotal to early cancer detection and treatment monitoring. Here, we use a spiral microchannel with inherent centrifugal forces for continuous, size-based separation of CTCs from blood (Dean Flow Fractionation (DFF)) which facilitates easy coupling with conventional downstream biological assays. Device performance was optimized using cancer cell lines (> 85% recovery), followed by clinical validation with positive CTCs enumeration in all samples from patients with metastatic lung cancer (n = 20; 5–88 CTCs per mL). The presence of CD133+ cells, a phenotypic marker characteristic of stem-like behavior in lung cancer cells was also identified in the isolated subpopulation of CTCs. The spiral biochip identifies and addresses key challenges of the next generation CTCs isolation assay including antibody independent isolation, high sensitivity and throughput (3 mL/hr); and single-step retrieval of viable CTCs. Singapore. National Research Foundation (Singapore MIT Alliance for Research and Technology's BioSystems and Micromechanics Inter-Disciplinary Research programme) Singapore. National Medical Research Council (grant NMRC 1225/2009) 2014-05-15T14:44:44Z 2014-05-15T14:44:44Z 2013-02 2012-10 Article http://purl.org/eprint/type/JournalArticle 2045-2322 http://hdl.handle.net/1721.1/86978 Hou, Han Wei, Majid Ebrahimi Warkiani, Bee Luan Khoo, Zi Rui Li, Ross A. Soo, Daniel Shao-Weng Tan, Wan-Teck Lim, Jongyoon Han, Ali Asgar S. Bhagat, and Chwee Teck Lim. “Isolation and Retrieval of Circulating Tumor Cells Using Centrifugal Forces.” Sci. Rep. 3 (February 12, 2013). https://orcid.org/0000-0001-7215-1439 en_US http://dx.doi.org/10.1038/srep01259 Scientific Reports Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License http://creativecommons.org/licenses/by-nc-nd/3.0/ application/pdf Nature Publishing Group Scientific Reports |
| spellingShingle | Hou, Han Wei Warkiani, Majid Ebrahimi Khoo, Bee Luan Li, Zi Rui Soo, Ross A. Tan, Daniel Shao-Weng Lim, Wan-Teck Han, Jongyoon Bhagat, Ali Asgar S. Lim, Chwee Teck Isolation and retrieval of circulating tumor cells using centrifugal forces |
| title | Isolation and retrieval of circulating tumor cells using centrifugal forces |
| title_full | Isolation and retrieval of circulating tumor cells using centrifugal forces |
| title_fullStr | Isolation and retrieval of circulating tumor cells using centrifugal forces |
| title_full_unstemmed | Isolation and retrieval of circulating tumor cells using centrifugal forces |
| title_short | Isolation and retrieval of circulating tumor cells using centrifugal forces |
| title_sort | isolation and retrieval of circulating tumor cells using centrifugal forces |
| url | http://hdl.handle.net/1721.1/86978 https://orcid.org/0000-0001-7215-1439 |
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