Nanostructured Optical Photonic Crystal Biosensor for HIV Viral Load Measurement
Detecting and quantifying biomarkers and viruses in biological samples have broad applications in early disease diagnosis and treatment monitoring. We have demonstrated a label-free optical sensing mechanism using nanostructured photonic crystals (PC) to capture and quantify intact viruses (HIV-1) f...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2014
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| Online Access: | http://hdl.handle.net/1721.1/87591 |
| _version_ | 1826198995134840832 |
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| author | Shafiee, Hadi Lidstone, Erich A. Jahangir, Muntasir Inci, Fatih Hanhauser, Emily Henrich, Timothy J. Kuritzkes, Daniel R. Cunningham, Brian T. Demirci, Utkan |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Shafiee, Hadi Lidstone, Erich A. Jahangir, Muntasir Inci, Fatih Hanhauser, Emily Henrich, Timothy J. Kuritzkes, Daniel R. Cunningham, Brian T. Demirci, Utkan |
| author_sort | Shafiee, Hadi |
| collection | MIT |
| description | Detecting and quantifying biomarkers and viruses in biological samples have broad applications in early disease diagnosis and treatment monitoring. We have demonstrated a label-free optical sensing mechanism using nanostructured photonic crystals (PC) to capture and quantify intact viruses (HIV-1) from biologically relevant samples. The nanostructured surface of the PC biosensor resonantly reflects a narrow wavelength band during illumination with a broadband light source. Surface-adsorbed biotarget induces a shift in the resonant Peak Wavelength Value (PWV) that is detectable with <10 pm wavelength resolution, enabling detection of both biomolecular layers and small number of viruses that sparsely populate the transducer surface. We have successfully captured and detected HIV-1 in serum and phosphate buffered saline (PBS) samples with viral loads ranging from 10[superscript 4] to 10[superscript 8] copies/mL. The surface density of immobilized biomolecular layers used in the sensor functionalization process, including 3-mercaptopropyltrimethoxysilane (3-MPS), N-gamma-Maleimidobutyryl-oxysuccinimide ester (GMBS), NeutrAvidin, anti-gp120, and bovine serum albumin (BSA) were also quantified by the PC biosensor. |
| first_indexed | 2024-09-23T11:12:47Z |
| format | Article |
| id | mit-1721.1/87591 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:12:47Z |
| publishDate | 2014 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/875912022-10-01T02:04:56Z Nanostructured Optical Photonic Crystal Biosensor for HIV Viral Load Measurement Shafiee, Hadi Lidstone, Erich A. Jahangir, Muntasir Inci, Fatih Hanhauser, Emily Henrich, Timothy J. Kuritzkes, Daniel R. Cunningham, Brian T. Demirci, Utkan Harvard University--MIT Division of Health Sciences and Technology Demirci, Utkan Detecting and quantifying biomarkers and viruses in biological samples have broad applications in early disease diagnosis and treatment monitoring. We have demonstrated a label-free optical sensing mechanism using nanostructured photonic crystals (PC) to capture and quantify intact viruses (HIV-1) from biologically relevant samples. The nanostructured surface of the PC biosensor resonantly reflects a narrow wavelength band during illumination with a broadband light source. Surface-adsorbed biotarget induces a shift in the resonant Peak Wavelength Value (PWV) that is detectable with <10 pm wavelength resolution, enabling detection of both biomolecular layers and small number of viruses that sparsely populate the transducer surface. We have successfully captured and detected HIV-1 in serum and phosphate buffered saline (PBS) samples with viral loads ranging from 10[superscript 4] to 10[superscript 8] copies/mL. The surface density of immobilized biomolecular layers used in the sensor functionalization process, including 3-mercaptopropyltrimethoxysilane (3-MPS), N-gamma-Maleimidobutyryl-oxysuccinimide ester (GMBS), NeutrAvidin, anti-gp120, and bovine serum albumin (BSA) were also quantified by the PC biosensor. National Institutes of Health (U.S.) (Award R01AI093282) National Institutes of Health (U.S.) (Award R01AI081534) National Institutes of Health (U.S.) (Award R21AI087107) National Institutes of Health (U.S.) (Award F32AI102590) 2014-05-30T17:40:53Z 2014-05-30T17:40:53Z 2014-02 2013-09 Article http://purl.org/eprint/type/JournalArticle 2045-2322 http://hdl.handle.net/1721.1/87591 Shafiee, Hadi, Erich A. Lidstone, Muntasir Jahangir, Fatih Inci, Emily Hanhauser, Timothy J. Henrich, Daniel R. Kuritzkes, Brian T. Cunningham, and Utkan Demirci. “Nanostructured Optical Photonic Crystal Biosensor for HIV Viral Load Measurement.” Sci. Rep. 4 (February 28, 2014). en_US http://dx.doi.org/10.1038/srep04116 Scientific Reports Creative Commons Attribution-Noncommercial http://creativecommons.org/licenses/by-nc-nd/3.0 application/pdf Nature Publishing Group Nature Publishing Group |
| spellingShingle | Shafiee, Hadi Lidstone, Erich A. Jahangir, Muntasir Inci, Fatih Hanhauser, Emily Henrich, Timothy J. Kuritzkes, Daniel R. Cunningham, Brian T. Demirci, Utkan Nanostructured Optical Photonic Crystal Biosensor for HIV Viral Load Measurement |
| title | Nanostructured Optical Photonic Crystal Biosensor for HIV Viral Load Measurement |
| title_full | Nanostructured Optical Photonic Crystal Biosensor for HIV Viral Load Measurement |
| title_fullStr | Nanostructured Optical Photonic Crystal Biosensor for HIV Viral Load Measurement |
| title_full_unstemmed | Nanostructured Optical Photonic Crystal Biosensor for HIV Viral Load Measurement |
| title_short | Nanostructured Optical Photonic Crystal Biosensor for HIV Viral Load Measurement |
| title_sort | nanostructured optical photonic crystal biosensor for hiv viral load measurement |
| url | http://hdl.handle.net/1721.1/87591 |
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