High-resolution three-dimensional optical coherence tomography imaging of kidney microanatomy ex vivo
Optical coherence tomography (OCT) is an emerging medical imaging technology that enables high-resolution, noninvasive, cross-sectional imaging of microstructure in biological tissues in situ and in real time. When combined with small-diameter catheters or needle probes, OCT offers a practical tool...
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| Format: | Article |
| Language: | en_US |
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SPIE
2014
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| Online Access: | http://hdl.handle.net/1721.1/87596 https://orcid.org/0000-0002-0828-4357 |
| _version_ | 1826212019868532736 |
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| author | Chen, Yu Andrews, Peter M. Aguirre, Aaron Dominic Schmitt, Joseph M. Fujimoto, James G. |
| author2 | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science |
| author_facet | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Chen, Yu Andrews, Peter M. Aguirre, Aaron Dominic Schmitt, Joseph M. Fujimoto, James G. |
| author_sort | Chen, Yu |
| collection | MIT |
| description | Optical coherence tomography (OCT) is an emerging medical imaging technology that enables high-resolution, noninvasive, cross-sectional imaging of microstructure in biological tissues in situ and in real time. When combined with small-diameter catheters or needle probes, OCT offers a practical tool for the minimally invasive imaging of living tissue morphology. We evaluate the ability of OCT to image normal kidneys and discriminate pathological changes in kidney structure. Both control and experimental preserved rat kidneys were examined ex vivo by using a high-resolution OCT imaging system equipped with a laser light source at 1.3-μm wavelength. This system has a resolution of 3.3 μm (depth) by 6 μm (transverse). OCT imaging produced cross-sectional and en face images that revealed the sizes and shapes of the uriniferous tubules and renal corpuscles. OCT data revealed significant changes in the uriniferous tubules of kidneys preserved following an ischemic or toxic (i.e., mercuric chloride) insult. OCT data was also rendered to produce informative three-dimensional (3-D) images of uriniferous tubules and renal corpuscles. The foregoing observations suggest that OCT can be a useful non-excisional, real-time modality for imaging pathological changes in donor kidney morphology prior to transplantation. |
| first_indexed | 2024-09-23T15:15:12Z |
| format | Article |
| id | mit-1721.1/87596 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:15:12Z |
| publishDate | 2014 |
| publisher | SPIE |
| record_format | dspace |
| spelling | mit-1721.1/875962022-09-29T13:37:56Z High-resolution three-dimensional optical coherence tomography imaging of kidney microanatomy ex vivo Chen, Yu Andrews, Peter M. Aguirre, Aaron Dominic Schmitt, Joseph M. Fujimoto, James G. Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Massachusetts Institute of Technology. Research Laboratory of Electronics Chen, Yu Aguirre, Aaron Dominic Fujimoto, James G. Optical coherence tomography (OCT) is an emerging medical imaging technology that enables high-resolution, noninvasive, cross-sectional imaging of microstructure in biological tissues in situ and in real time. When combined with small-diameter catheters or needle probes, OCT offers a practical tool for the minimally invasive imaging of living tissue morphology. We evaluate the ability of OCT to image normal kidneys and discriminate pathological changes in kidney structure. Both control and experimental preserved rat kidneys were examined ex vivo by using a high-resolution OCT imaging system equipped with a laser light source at 1.3-μm wavelength. This system has a resolution of 3.3 μm (depth) by 6 μm (transverse). OCT imaging produced cross-sectional and en face images that revealed the sizes and shapes of the uriniferous tubules and renal corpuscles. OCT data revealed significant changes in the uriniferous tubules of kidneys preserved following an ischemic or toxic (i.e., mercuric chloride) insult. OCT data was also rendered to produce informative three-dimensional (3-D) images of uriniferous tubules and renal corpuscles. The foregoing observations suggest that OCT can be a useful non-excisional, real-time modality for imaging pathological changes in donor kidney morphology prior to transplantation. National Institutes of Health (U.S.) (R01-CA75289-10) National Institutes of Health (U.S.) (R01-EY11289-21) United States. Air Force Office of Scientific Research (FA9550-040-1-001) United States. Air Force Office of Scientific Research (FA9550-010-0046) National Science Foundation (U.S.) (BES-0522845) National Science Foundation (U.S.) (ECS-0501478) National Kidney Foundation 2014-05-30T19:19:17Z 2014-05-30T19:19:17Z 2007-05 2007-03 Article http://purl.org/eprint/type/JournalArticle 10833668 1560-2281 http://hdl.handle.net/1721.1/87596 Chen, Yu, Peter M. Andrews, Aaron D. Aguirre, Joseph M. Schmitt, and James G. Fujimoto. “High-Resolution Three-Dimensional Optical Coherence Tomography Imaging of Kidney Microanatomy Ex Vivo.” Journal of Biomedical Optics 12, no. 3 (2007): 034008. © 2007 SPIE https://orcid.org/0000-0002-0828-4357 en_US http://dx.doi.org/10.1117/1.2736421 Journal of Biomedical Optics Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf SPIE SPIE |
| spellingShingle | Chen, Yu Andrews, Peter M. Aguirre, Aaron Dominic Schmitt, Joseph M. Fujimoto, James G. High-resolution three-dimensional optical coherence tomography imaging of kidney microanatomy ex vivo |
| title | High-resolution three-dimensional optical coherence tomography imaging of kidney microanatomy ex vivo |
| title_full | High-resolution three-dimensional optical coherence tomography imaging of kidney microanatomy ex vivo |
| title_fullStr | High-resolution three-dimensional optical coherence tomography imaging of kidney microanatomy ex vivo |
| title_full_unstemmed | High-resolution three-dimensional optical coherence tomography imaging of kidney microanatomy ex vivo |
| title_short | High-resolution three-dimensional optical coherence tomography imaging of kidney microanatomy ex vivo |
| title_sort | high resolution three dimensional optical coherence tomography imaging of kidney microanatomy ex vivo |
| url | http://hdl.handle.net/1721.1/87596 https://orcid.org/0000-0002-0828-4357 |
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