Doppler optical coherence microscopy for studies of cochlear mechanics
The possibility of measuring subnanometer motions with micron scale spatial resolution in the intact mammalian cochlea using Doppler optical coherence microscopy (DOCM) is demonstrated. A novel DOCM system is described that uses two acousto-optic modulators to generate a stable 500-kHz heterodyne fr...
| Main Authors: | , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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SPIE
2014
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| Online Access: | http://hdl.handle.net/1721.1/87598 https://orcid.org/0000-0001-6309-0910 |
| _version_ | 1826216341124677632 |
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| author | Hong, Stanley S. Freeman, Dennis M. |
| author2 | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science |
| author_facet | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Hong, Stanley S. Freeman, Dennis M. |
| author_sort | Hong, Stanley S. |
| collection | MIT |
| description | The possibility of measuring subnanometer motions with micron scale spatial resolution in the intact mammalian cochlea using Doppler optical coherence microscopy (DOCM) is demonstrated. A novel DOCM system is described that uses two acousto-optic modulators to generate a stable 500-kHz heterodyne frequency. Images and motion measurements are obtained using phase-resolved analysis of the interference signal. The DOCM system permits imaging with micron-scale resolution and 85-dB sensitivity and motion measurements with 100-kHz bandwidth, directional discrimination, and 30-pm/Hz[superscript 0.5] noise floor. Images and motion measurements are presented that demonstrate the ability to resolve motions of structures of interest in a mammalian cochlea in vitro including the basilar membrane, reticular lamina, tectorial membrane, and outer hair cells. |
| first_indexed | 2024-09-23T16:46:05Z |
| format | Article |
| id | mit-1721.1/87598 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T16:46:05Z |
| publishDate | 2014 |
| publisher | SPIE |
| record_format | dspace |
| spelling | mit-1721.1/875982022-10-03T08:09:34Z Doppler optical coherence microscopy for studies of cochlear mechanics Hong, Stanley S. Freeman, Dennis M. Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Massachusetts Institute of Technology. Research Laboratory of Electronics Hong, Stanley S. Freeman, Dennis M. The possibility of measuring subnanometer motions with micron scale spatial resolution in the intact mammalian cochlea using Doppler optical coherence microscopy (DOCM) is demonstrated. A novel DOCM system is described that uses two acousto-optic modulators to generate a stable 500-kHz heterodyne frequency. Images and motion measurements are obtained using phase-resolved analysis of the interference signal. The DOCM system permits imaging with micron-scale resolution and 85-dB sensitivity and motion measurements with 100-kHz bandwidth, directional discrimination, and 30-pm/Hz[superscript 0.5] noise floor. Images and motion measurements are presented that demonstrate the ability to resolve motions of structures of interest in a mammalian cochlea in vitro including the basilar membrane, reticular lamina, tectorial membrane, and outer hair cells. National Institutes of Health (U.S.) (Grant 1-R21-DC007111-01) 2014-05-30T19:59:44Z 2014-05-30T19:59:44Z 2006-10 2006-05 Article http://purl.org/eprint/type/JournalArticle 10833668 1560-2281 http://hdl.handle.net/1721.1/87598 Hong, Stanley S., and Dennis M. Freeman. “Doppler Optical Coherence Microscopy for Studies of Cochlear Mechanics.” Journal of Biomedical Optics 11, no. 5 (2006): 054014. © 2006 SPIE https://orcid.org/0000-0001-6309-0910 en_US http://dx.doi.org/10.1117/1.2358702 Journal of Biomedical Optics Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf SPIE SPIE |
| spellingShingle | Hong, Stanley S. Freeman, Dennis M. Doppler optical coherence microscopy for studies of cochlear mechanics |
| title | Doppler optical coherence microscopy for studies of cochlear mechanics |
| title_full | Doppler optical coherence microscopy for studies of cochlear mechanics |
| title_fullStr | Doppler optical coherence microscopy for studies of cochlear mechanics |
| title_full_unstemmed | Doppler optical coherence microscopy for studies of cochlear mechanics |
| title_short | Doppler optical coherence microscopy for studies of cochlear mechanics |
| title_sort | doppler optical coherence microscopy for studies of cochlear mechanics |
| url | http://hdl.handle.net/1721.1/87598 https://orcid.org/0000-0001-6309-0910 |
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