Effect of probe pressure on cervical fluorescence spectroscopy measurements
Fluorescence spectroscopy is a promising technology for detection of epithelial precancers and cancers. While age and menopausal status influence measurements in the cervix, other variables do not significantly affect the diagnosis. In this study we examine probe pressure as a variable. A fiber opti...
| Hoofdauteurs: | , , , , , , |
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| Formaat: | Artikel |
| Taal: | en_US |
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SPIE
2014
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| Online toegang: | http://hdl.handle.net/1721.1/87646 |
| _version_ | 1826210290121834496 |
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| author | Nath, Audrey Rivoire, Kelley Chang, Sung Cox, Dennis Atkinson, E. Neely Follen, Michele Richards-Kortum, Rebecca |
| author2 | Massachusetts Institute of Technology. Department of Physics |
| author_facet | Massachusetts Institute of Technology. Department of Physics Nath, Audrey Rivoire, Kelley Chang, Sung Cox, Dennis Atkinson, E. Neely Follen, Michele Richards-Kortum, Rebecca |
| author_sort | Nath, Audrey |
| collection | MIT |
| description | Fluorescence spectroscopy is a promising technology for detection of epithelial precancers and cancers. While age and menopausal status influence measurements in the cervix, other variables do not significantly affect the diagnosis. In this study we examine probe pressure as a variable. A fiber optic probe to measure fluorescence spectra at different calibrated levels of pressure was designed and tested. A pilot study was conducted measuring fluorescence excitation emission matrices in 20 patients at light, medium, and firm pressure. Spectroscopic data were pre-processed and analyzed to compare mean peak intensities as a function of pressure. Further statistical analyses tested for differences in intensities at each excitation/emission wavelength pair. Four providers made measurements from 41 sites; 33 yielded good quality spectroscopic data (22 squamous normal, 7 squamous abnormal, 3 columnar normal, 1 transformation zone) from 17 of 20 patients. At all pressure levels, abnormal tissue showed less fluorescence intensity than normal tissue, and post-menopausal patients showed higher fluorescence intensity than premenopausal patients, consistent with previous analyses. A permutation analysis suggests that pressure does not significantly affect fluorescence intensity or lineshape. While other studies are needed to confirm these findings, this study suggests that fluorescence spectroscopy is a robust technology likely not influenced by fiber optic probe pressure. |
| first_indexed | 2024-09-23T14:47:27Z |
| format | Article |
| id | mit-1721.1/87646 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:47:27Z |
| publishDate | 2014 |
| publisher | SPIE |
| record_format | dspace |
| spelling | mit-1721.1/876462022-10-01T22:28:56Z Effect of probe pressure on cervical fluorescence spectroscopy measurements Nath, Audrey Rivoire, Kelley Chang, Sung Cox, Dennis Atkinson, E. Neely Follen, Michele Richards-Kortum, Rebecca Massachusetts Institute of Technology. Department of Physics Rivoire, Kelley Fluorescence spectroscopy is a promising technology for detection of epithelial precancers and cancers. While age and menopausal status influence measurements in the cervix, other variables do not significantly affect the diagnosis. In this study we examine probe pressure as a variable. A fiber optic probe to measure fluorescence spectra at different calibrated levels of pressure was designed and tested. A pilot study was conducted measuring fluorescence excitation emission matrices in 20 patients at light, medium, and firm pressure. Spectroscopic data were pre-processed and analyzed to compare mean peak intensities as a function of pressure. Further statistical analyses tested for differences in intensities at each excitation/emission wavelength pair. Four providers made measurements from 41 sites; 33 yielded good quality spectroscopic data (22 squamous normal, 7 squamous abnormal, 3 columnar normal, 1 transformation zone) from 17 of 20 patients. At all pressure levels, abnormal tissue showed less fluorescence intensity than normal tissue, and post-menopausal patients showed higher fluorescence intensity than premenopausal patients, consistent with previous analyses. A permutation analysis suggests that pressure does not significantly affect fluorescence intensity or lineshape. While other studies are needed to confirm these findings, this study suggests that fluorescence spectroscopy is a robust technology likely not influenced by fiber optic probe pressure. National Cancer Institute (U.S.) (Program Project Grant PO1 CA 82710-04) 2014-06-05T14:53:52Z 2014-06-05T14:53:52Z 2004-04 2004-02 Article http://purl.org/eprint/type/JournalArticle 10833668 1560-2281 http://hdl.handle.net/1721.1/87646 Nath, Audrey, Kelley Rivoire, Sung Chang, Dennis Cox, E. Neely Atkinson, Michele Follen, and Rebecca Richards-Kortum. “Effect of Probe Pressure on Cervical Fluorescence Spectroscopy Measurements.” Journal of Biomedical Optics 9, no. 3 (2004): 523. © 2004 Society of Photo-Optical Instrumentation Engineers en_US http://dx.doi.org/10.1117/1.1695562 Journal of Biomedical Optics Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf SPIE SPIE |
| spellingShingle | Nath, Audrey Rivoire, Kelley Chang, Sung Cox, Dennis Atkinson, E. Neely Follen, Michele Richards-Kortum, Rebecca Effect of probe pressure on cervical fluorescence spectroscopy measurements |
| title | Effect of probe pressure on cervical fluorescence spectroscopy measurements |
| title_full | Effect of probe pressure on cervical fluorescence spectroscopy measurements |
| title_fullStr | Effect of probe pressure on cervical fluorescence spectroscopy measurements |
| title_full_unstemmed | Effect of probe pressure on cervical fluorescence spectroscopy measurements |
| title_short | Effect of probe pressure on cervical fluorescence spectroscopy measurements |
| title_sort | effect of probe pressure on cervical fluorescence spectroscopy measurements |
| url | http://hdl.handle.net/1721.1/87646 |
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