High-resolution whole organ imaging using two-photon tissue cytometry
Three-dimensional (3-D) tissue imaging offers substantial benefits to a wide range of biomedical investigations from cardiovascular biology, diabetes, Alzheimer’s disease to cancer. Two-photon tissue cytometry is a novel technique based on high-speed multiphoton microscopy coupled with automated his...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | en_US |
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SPIE
2014
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| Online Access: | http://hdl.handle.net/1721.1/87650 https://orcid.org/0000-0003-4698-6488 |
| _version_ | 1826197773242859520 |
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| author | Ragan, Timothy Sylvan, Jeremy D. Kim, Ki Hean Huang, Hayden Bahlmann, Karsten Lee, Richard T. So, Peter T. C. |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Ragan, Timothy Sylvan, Jeremy D. Kim, Ki Hean Huang, Hayden Bahlmann, Karsten Lee, Richard T. So, Peter T. C. |
| author_sort | Ragan, Timothy |
| collection | MIT |
| description | Three-dimensional (3-D) tissue imaging offers substantial benefits to a wide range of biomedical investigations from cardiovascular biology, diabetes, Alzheimer’s disease to cancer. Two-photon tissue cytometry is a novel technique based on high-speed multiphoton microscopy coupled with automated histological sectioning, which can quantify tissue morphology and physiology throughout entire organs with subcellular resolution. Furthermore, two-photon tissue cytometry offers all the benefits of fluorescence-based approaches including high specificity and sensitivity and appropriateness for molecular imaging of gene and protein expression. We use two-photon tissue cytometry to image an entire mouse heart at subcellular resolution to quantify the 3-D morphology of cardiac microvasculature and myocyte morphology spanning almost five orders of magnitude in length scales. |
| first_indexed | 2024-09-23T10:52:56Z |
| format | Article |
| id | mit-1721.1/87650 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:52:56Z |
| publishDate | 2014 |
| publisher | SPIE |
| record_format | dspace |
| spelling | mit-1721.1/876502022-09-30T23:40:04Z High-resolution whole organ imaging using two-photon tissue cytometry Ragan, Timothy Sylvan, Jeremy D. Kim, Ki Hean Huang, Hayden Bahlmann, Karsten Lee, Richard T. So, Peter T. C. Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Mechanical Engineering Ragan, Timothy Bahlmann, Karsten So, Peter T. C. Three-dimensional (3-D) tissue imaging offers substantial benefits to a wide range of biomedical investigations from cardiovascular biology, diabetes, Alzheimer’s disease to cancer. Two-photon tissue cytometry is a novel technique based on high-speed multiphoton microscopy coupled with automated histological sectioning, which can quantify tissue morphology and physiology throughout entire organs with subcellular resolution. Furthermore, two-photon tissue cytometry offers all the benefits of fluorescence-based approaches including high specificity and sensitivity and appropriateness for molecular imaging of gene and protein expression. We use two-photon tissue cytometry to image an entire mouse heart at subcellular resolution to quantify the 3-D morphology of cardiac microvasculature and myocyte morphology spanning almost five orders of magnitude in length scales. National Institutes of Health (U.S.) (R43 CA097670-01) National Institutes of Health (U.S.) (R44 CA097670-02) 2014-06-05T15:46:25Z 2014-06-05T15:46:25Z 2007-02 2006-10 Article http://purl.org/eprint/type/JournalArticle 10833668 1560-2281 http://hdl.handle.net/1721.1/87650 Ragan, Timothy, Jeremy D. Sylvan, Ki Hean Kim, Hayden Huang, Karsten Bahlmann, Richard T. Lee, and Peter T. C. So. “High-Resolution Whole Organ Imaging Using Two-Photon Tissue Cytometry.” Journal of Biomedical Optics 12, no. 1 (2007): 014015. © 2007 Society of Photo-Optical Instrumentation Engineers https://orcid.org/0000-0003-4698-6488 en_US http://dx.doi.org/10.1117/1.2435626 Journal of Biomedical Optics Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf SPIE SPIE |
| spellingShingle | Ragan, Timothy Sylvan, Jeremy D. Kim, Ki Hean Huang, Hayden Bahlmann, Karsten Lee, Richard T. So, Peter T. C. High-resolution whole organ imaging using two-photon tissue cytometry |
| title | High-resolution whole organ imaging using two-photon tissue cytometry |
| title_full | High-resolution whole organ imaging using two-photon tissue cytometry |
| title_fullStr | High-resolution whole organ imaging using two-photon tissue cytometry |
| title_full_unstemmed | High-resolution whole organ imaging using two-photon tissue cytometry |
| title_short | High-resolution whole organ imaging using two-photon tissue cytometry |
| title_sort | high resolution whole organ imaging using two photon tissue cytometry |
| url | http://hdl.handle.net/1721.1/87650 https://orcid.org/0000-0003-4698-6488 |
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