Retinal response of Macaca mulatta to picosecond laser pulses of varying energy and spot size
We investigate the relationship between the laser beam at the retina (spot size) and the extent of retinal injury from single ultrashort laser pulses. From previous studies it is believed that the retinal effect of single 3-ps laser pulses should vary in extent and location, depending on the occurre...
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| Format: | Article |
| Language: | en_US |
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SPIE
2014
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| Online Access: | http://hdl.handle.net/1721.1/87651 https://orcid.org/0000-0002-0828-4357 |
| _version_ | 1811070724937351168 |
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| author | Roach, William P. Cain, Clarence P. Narayan, Drew G. Noojin, Gary D. Boppart, Stephen A. Birngruber, Reginald Fujimoto, James G. Toth, Cynthia A. |
| author2 | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science |
| author_facet | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Roach, William P. Cain, Clarence P. Narayan, Drew G. Noojin, Gary D. Boppart, Stephen A. Birngruber, Reginald Fujimoto, James G. Toth, Cynthia A. |
| author_sort | Roach, William P. |
| collection | MIT |
| description | We investigate the relationship between the laser beam at the retina (spot size) and the extent of retinal injury from single ultrashort laser pulses. From previous studies it is believed that the retinal effect of single 3-ps laser pulses should vary in extent and location, depending on the occurrence of laser-induced breakdown (LIB) at the site of laser delivery. Single 3-ps pulses of 580-nm laser energy are delivered over a range of spot sizes to the retina of Macaca mulatta. The retinal response is captured sequentially with optical coherence tomography (OCT). The in vivo OCT images and the extent of pathology on final microscopic sections of the laser site are compared. With delivery of a laser pulse with peak irradiance greater than that required for LIB, OCT and light micrographs demonstrate inner retinal injury with many intraretinal and/or vitreous hemorrhages. In contrast, broad outer retinal injury with minimal to no choriocapillaris effect is seen after delivery of laser pulses to a larger retinal area (60 to 300 μm diam) when peak irradiance is less than that required for LIB. The broader lesions extend into the inner retina when higher energy delivery produces intraretinal injury. Microscopic examination of stained fixed tissues provide better resolution of retinal morphology than OCT. OCT provides less resolution but could be guided over an in vivo, visible retinal lesion for repeated sampling over time during the evolution of the lesion formation. For 3-ps visible wavelength laser pulses, varying the spot size and laser energy directly affects the extent of retinal injury. This again is believed to be partly due to the onset of LIB, as seen in previous studies. Spot-size dependence should be considered when comparing studies of retinal effects or when pursuing a specific retinal effect from ultrashort laser pulses. |
| first_indexed | 2024-09-23T08:40:34Z |
| format | Article |
| id | mit-1721.1/87651 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T08:40:34Z |
| publishDate | 2014 |
| publisher | SPIE |
| record_format | dspace |
| spelling | mit-1721.1/876512022-09-30T10:25:49Z Retinal response of Macaca mulatta to picosecond laser pulses of varying energy and spot size Roach, William P. Cain, Clarence P. Narayan, Drew G. Noojin, Gary D. Boppart, Stephen A. Birngruber, Reginald Fujimoto, James G. Toth, Cynthia A. Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Fujimoto, James G. We investigate the relationship between the laser beam at the retina (spot size) and the extent of retinal injury from single ultrashort laser pulses. From previous studies it is believed that the retinal effect of single 3-ps laser pulses should vary in extent and location, depending on the occurrence of laser-induced breakdown (LIB) at the site of laser delivery. Single 3-ps pulses of 580-nm laser energy are delivered over a range of spot sizes to the retina of Macaca mulatta. The retinal response is captured sequentially with optical coherence tomography (OCT). The in vivo OCT images and the extent of pathology on final microscopic sections of the laser site are compared. With delivery of a laser pulse with peak irradiance greater than that required for LIB, OCT and light micrographs demonstrate inner retinal injury with many intraretinal and/or vitreous hemorrhages. In contrast, broad outer retinal injury with minimal to no choriocapillaris effect is seen after delivery of laser pulses to a larger retinal area (60 to 300 μm diam) when peak irradiance is less than that required for LIB. The broader lesions extend into the inner retina when higher energy delivery produces intraretinal injury. Microscopic examination of stained fixed tissues provide better resolution of retinal morphology than OCT. OCT provides less resolution but could be guided over an in vivo, visible retinal lesion for repeated sampling over time during the evolution of the lesion formation. For 3-ps visible wavelength laser pulses, varying the spot size and laser energy directly affects the extent of retinal injury. This again is believed to be partly due to the onset of LIB, as seen in previous studies. Spot-size dependence should be considered when comparing studies of retinal effects or when pursuing a specific retinal effect from ultrashort laser pulses. United States. Air Force Office of Scientific Research (Grant F49620-95-1-0266) United States. Air Force Office of Scientific Research (Grant 2312AA-92AL014) United States. Air Force Office of Scientific Research (Armstrong Laboratory Contract F33615-92-C-0017) 2014-06-05T16:01:48Z 2014-06-05T16:01:48Z 2004-11 2004-03 Article http://purl.org/eprint/type/JournalArticle 10833668 1560-2281 http://hdl.handle.net/1721.1/87651 Roach, William P., Clarence P. Cain, Drew G. Narayan, Gary D. Noojin, Stephen A. Boppart, Reginald Birngruber, James G. Fujimoto, and Cynthia A. Toth. “Retinal Response of Macaca Mulatta to Picosecond Laser Pulses of Varying Energy and Spot Size.” Journal of Biomedical Optics 9, no. 6 (2004): 1288. © 2004 Society of Photo-Optical Instrumentation Engineers https://orcid.org/0000-0002-0828-4357 en_US http://dx.doi.org/10.1117/1.1805554 Journal of Biomedical Optics Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf SPIE SPIE |
| spellingShingle | Roach, William P. Cain, Clarence P. Narayan, Drew G. Noojin, Gary D. Boppart, Stephen A. Birngruber, Reginald Fujimoto, James G. Toth, Cynthia A. Retinal response of Macaca mulatta to picosecond laser pulses of varying energy and spot size |
| title | Retinal response of Macaca mulatta to picosecond laser pulses of varying energy and spot size |
| title_full | Retinal response of Macaca mulatta to picosecond laser pulses of varying energy and spot size |
| title_fullStr | Retinal response of Macaca mulatta to picosecond laser pulses of varying energy and spot size |
| title_full_unstemmed | Retinal response of Macaca mulatta to picosecond laser pulses of varying energy and spot size |
| title_short | Retinal response of Macaca mulatta to picosecond laser pulses of varying energy and spot size |
| title_sort | retinal response of macaca mulatta to picosecond laser pulses of varying energy and spot size |
| url | http://hdl.handle.net/1721.1/87651 https://orcid.org/0000-0002-0828-4357 |
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