Prospective grading of neoplastic change in rat esophagus epithelium using angle-resolved low-coherence interferometry
Angle-resolved low-coherence interferometry (a/LCI) is used to obtain quantitative, depth-resolved nuclear morphology measurements. We compare the average diameter and texture of cell nuclei in rat esophagus epithelial tissue to grading criteria established in a previous a/LCI study to prospectively...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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SPIE
2014
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| Online Access: | http://hdl.handle.net/1721.1/87657 |
| _version_ | 1826194399431753728 |
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| author | Wax, Adam Pyhtila, John W. Graf, Robert N. Nines, Ronald Boone, Charles W. Feld, Michael S. Steele, Vernon E. Stoner, Gary D. Dasari, Ramachandra Rao |
| author2 | Massachusetts Institute of Technology. Department of Chemistry |
| author_facet | Massachusetts Institute of Technology. Department of Chemistry Wax, Adam Pyhtila, John W. Graf, Robert N. Nines, Ronald Boone, Charles W. Feld, Michael S. Steele, Vernon E. Stoner, Gary D. Dasari, Ramachandra Rao |
| author_sort | Wax, Adam |
| collection | MIT |
| description | Angle-resolved low-coherence interferometry (a/LCI) is used to obtain quantitative, depth-resolved nuclear morphology measurements. We compare the average diameter and texture of cell nuclei in rat esophagus epithelial tissue to grading criteria established in a previous a/LCI study to prospectively grade neoplastic progression. We exploit the depth resolution of a/LCI to exclusively examine the basal layer of the epithelium, approximately 50 to 100 μm beneath the tissue surface, without the need for exogenous contrast agents, tissue sectioning, or fixation. The results of two studies are presented that compare the performance of two a/LCI modalities. Overall, the combined studies show 91% sensitivity and 97% specificity for detecting dysplasia, using histopathology as the standard. In addition, the studies enable the effects of dietary chemopreventive agents, difluoromethylornithine (DFMO) and curcumin, to be assessed by observing modulation in the incidence of neoplastic change. We demonstrate that a/LCI is highly effective for monitoring neoplastic change and can be applied to assessing the efficacy of chemopreventive agents in the rat esophagus. |
| first_indexed | 2024-09-23T09:55:04Z |
| format | Article |
| id | mit-1721.1/87657 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:55:04Z |
| publishDate | 2014 |
| publisher | SPIE |
| record_format | dspace |
| spelling | mit-1721.1/876572022-09-30T17:42:02Z Prospective grading of neoplastic change in rat esophagus epithelium using angle-resolved low-coherence interferometry Wax, Adam Pyhtila, John W. Graf, Robert N. Nines, Ronald Boone, Charles W. Feld, Michael S. Steele, Vernon E. Stoner, Gary D. Dasari, Ramachandra Rao Massachusetts Institute of Technology. Department of Chemistry Massachusetts Institute of Technology. Department of Physics Massachusetts Institute of Technology. Spectroscopy Laboratory Dasari, Ramachandra Rao Feld, Michael S. Angle-resolved low-coherence interferometry (a/LCI) is used to obtain quantitative, depth-resolved nuclear morphology measurements. We compare the average diameter and texture of cell nuclei in rat esophagus epithelial tissue to grading criteria established in a previous a/LCI study to prospectively grade neoplastic progression. We exploit the depth resolution of a/LCI to exclusively examine the basal layer of the epithelium, approximately 50 to 100 μm beneath the tissue surface, without the need for exogenous contrast agents, tissue sectioning, or fixation. The results of two studies are presented that compare the performance of two a/LCI modalities. Overall, the combined studies show 91% sensitivity and 97% specificity for detecting dysplasia, using histopathology as the standard. In addition, the studies enable the effects of dietary chemopreventive agents, difluoromethylornithine (DFMO) and curcumin, to be assessed by observing modulation in the incidence of neoplastic change. We demonstrate that a/LCI is highly effective for monitoring neoplastic change and can be applied to assessing the efficacy of chemopreventive agents in the rat esophagus. National Center for Research Resources (U.S.) (Grant P41-RR02594) National Cancer Institute (U.S.) (Grant NCI-CN15011-72) 2014-06-05T17:39:49Z 2014-06-05T17:39:49Z 2005-10 2005-04 Article http://purl.org/eprint/type/JournalArticle 10833668 1560-2281 http://hdl.handle.net/1721.1/87657 Wax, Adam, John W. Pyhtila, Robert N. Graf, Ronald Nines, Charles W. Boone, Ramachandra R. Dasari, Michael S. Feld, Vernon E. Steele, and Gary D. Stoner. “Prospective Grading of Neoplastic Change in Rat Esophagus Epithelium Using Angle-Resolved Low-Coherence Interferometry.” Journal of Biomedical Optics 10, no. 5 (2005): 051604. © 2005 Society of Photo-Optical Instrumentation Engineers en_US http://dx.doi.org/10.1117/1.2102767 Journal of Biomedical Optics Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf SPIE SPIE |
| spellingShingle | Wax, Adam Pyhtila, John W. Graf, Robert N. Nines, Ronald Boone, Charles W. Feld, Michael S. Steele, Vernon E. Stoner, Gary D. Dasari, Ramachandra Rao Prospective grading of neoplastic change in rat esophagus epithelium using angle-resolved low-coherence interferometry |
| title | Prospective grading of neoplastic change in rat esophagus epithelium using angle-resolved low-coherence interferometry |
| title_full | Prospective grading of neoplastic change in rat esophagus epithelium using angle-resolved low-coherence interferometry |
| title_fullStr | Prospective grading of neoplastic change in rat esophagus epithelium using angle-resolved low-coherence interferometry |
| title_full_unstemmed | Prospective grading of neoplastic change in rat esophagus epithelium using angle-resolved low-coherence interferometry |
| title_short | Prospective grading of neoplastic change in rat esophagus epithelium using angle-resolved low-coherence interferometry |
| title_sort | prospective grading of neoplastic change in rat esophagus epithelium using angle resolved low coherence interferometry |
| url | http://hdl.handle.net/1721.1/87657 |
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