Recombineering strategies for developing next generation BAC transgenic tools for optogenetics and beyond
The development and application of diverse BAC transgenic rodent lines has enabled rapid progress for precise molecular targeting of genetically-defined cell types in the mammalian central nervous system. These transgenic tools have played a central role in the optogenetic revolution in neuroscience...
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| Format: | Article |
| Language: | en_US |
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Frontiers Research Foundation
2014
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| Online Access: | http://hdl.handle.net/1721.1/88112 https://orcid.org/0000-0002-8021-277X |
| _version_ | 1826217496576786432 |
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| author | Feng, Guoping Ting, Jonathan Thomas |
| author2 | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences |
| author_facet | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Feng, Guoping Ting, Jonathan Thomas |
| author_sort | Feng, Guoping |
| collection | MIT |
| description | The development and application of diverse BAC transgenic rodent lines has enabled rapid progress for precise molecular targeting of genetically-defined cell types in the mammalian central nervous system. These transgenic tools have played a central role in the optogenetic revolution in neuroscience. Indeed, an overwhelming proportion of studies in this field have made use of BAC transgenic Cre driver lines to achieve targeted expression of optogenetic probes in the brain. In addition, several BAC transgenic mouse lines have been established for direct cell-type specific expression of Channelrhodopsin-2 (ChR2). While the benefits of these new tools largely outweigh any accompanying challenges, many available BAC transgenic lines may suffer from confounds due in part to increased gene dosage of one or more “extra” genes contained within the large BAC DNA sequences. Here we discuss this under-appreciated issue and propose strategies for developing the next generation of BAC transgenic lines that are devoid of extra genes. Furthermore, we provide evidence that these strategies are simple, reproducible, and do not disrupt the intended cell-type specific transgene expression patterns for several distinct BAC clones. These strategies may be widely implemented for improved BAC transgenesis across diverse disciplines. |
| first_indexed | 2024-09-23T17:04:35Z |
| format | Article |
| id | mit-1721.1/88112 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T17:04:35Z |
| publishDate | 2014 |
| publisher | Frontiers Research Foundation |
| record_format | dspace |
| spelling | mit-1721.1/881122022-10-03T10:12:11Z Recombineering strategies for developing next generation BAC transgenic tools for optogenetics and beyond Feng, Guoping Ting, Jonathan Thomas Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences McGovern Institute for Brain Research at MIT Ting, Jonathan Thomas Feng, Guoping The development and application of diverse BAC transgenic rodent lines has enabled rapid progress for precise molecular targeting of genetically-defined cell types in the mammalian central nervous system. These transgenic tools have played a central role in the optogenetic revolution in neuroscience. Indeed, an overwhelming proportion of studies in this field have made use of BAC transgenic Cre driver lines to achieve targeted expression of optogenetic probes in the brain. In addition, several BAC transgenic mouse lines have been established for direct cell-type specific expression of Channelrhodopsin-2 (ChR2). While the benefits of these new tools largely outweigh any accompanying challenges, many available BAC transgenic lines may suffer from confounds due in part to increased gene dosage of one or more “extra” genes contained within the large BAC DNA sequences. Here we discuss this under-appreciated issue and propose strategies for developing the next generation of BAC transgenic lines that are devoid of extra genes. Furthermore, we provide evidence that these strategies are simple, reproducible, and do not disrupt the intended cell-type specific transgene expression patterns for several distinct BAC clones. These strategies may be widely implemented for improved BAC transgenesis across diverse disciplines. Brain and Behavior Research Foundation (Young Investigator Award) National Institutes of Health (U.S.). Ruth L. Kirschstein National Research Service Award (F32-MH084460) 2014-06-30T12:18:54Z 2014-06-30T12:18:54Z 2014-04 2014-01 Article http://purl.org/eprint/type/JournalArticle 1662-5153 http://hdl.handle.net/1721.1/88112 Ting, Jonathan T., and Guoping Feng. “Recombineering Strategies for Developing Next Generation BAC Transgenic Tools for Optogenetics and Beyond.” Front. Behav. Neurosci. 8 (April 3, 2014). https://orcid.org/0000-0002-8021-277X en_US http://dx.doi.org/10.3389/fnbeh.2014.00111 Frontiers in Behavioral Neuroscience Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Frontiers Research Foundation Frontiers Research Foundation |
| spellingShingle | Feng, Guoping Ting, Jonathan Thomas Recombineering strategies for developing next generation BAC transgenic tools for optogenetics and beyond |
| title | Recombineering strategies for developing next generation BAC transgenic tools for optogenetics and beyond |
| title_full | Recombineering strategies for developing next generation BAC transgenic tools for optogenetics and beyond |
| title_fullStr | Recombineering strategies for developing next generation BAC transgenic tools for optogenetics and beyond |
| title_full_unstemmed | Recombineering strategies for developing next generation BAC transgenic tools for optogenetics and beyond |
| title_short | Recombineering strategies for developing next generation BAC transgenic tools for optogenetics and beyond |
| title_sort | recombineering strategies for developing next generation bac transgenic tools for optogenetics and beyond |
| url | http://hdl.handle.net/1721.1/88112 https://orcid.org/0000-0002-8021-277X |
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