Portable microfluidic chip for detection of Escherichia coli in produce and blood
Pathogenic agents can lead to severe clinical outcomes such as food poisoning, infection of open wounds, particularly in burn injuries and sepsis. Rapid detection of these pathogens can monitor these infections in a timely manner improving clinical outcomes. Conventional bacterial detection methods,...
| Principais autores: | , , , , , , , , , |
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| Outros Autores: | |
| Formato: | Artigo |
| Idioma: | en_US |
| Publicado em: |
Dove Medical Press
2014
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| Acesso em linha: | http://hdl.handle.net/1721.1/88155 |
| _version_ | 1826189066028187648 |
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| author | Wang, ShuQi Inci, Fatih Chaunzwa, Tafadzwa L. Ramanujam, Ajay Vasudevan, Aishwarya Subramanian, Sathya Ip, Alexander Chi Fai Sridharan, Banupriya Gurkan, Umut Atakan Demirci, Utkan |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Wang, ShuQi Inci, Fatih Chaunzwa, Tafadzwa L. Ramanujam, Ajay Vasudevan, Aishwarya Subramanian, Sathya Ip, Alexander Chi Fai Sridharan, Banupriya Gurkan, Umut Atakan Demirci, Utkan |
| author_sort | Wang, ShuQi |
| collection | MIT |
| description | Pathogenic agents can lead to severe clinical outcomes such as food poisoning, infection of open wounds, particularly in burn injuries and sepsis. Rapid detection of these pathogens can monitor these infections in a timely manner improving clinical outcomes. Conventional bacterial detection methods, such as agar plate culture or polymerase chain reaction, are time-consuming and dependent on complex and expensive instruments, which are not suitable for point-of-care (POC) settings. Therefore, there is an unmet need to develop a simple, rapid method for detection of pathogens such as Escherichia coli. Here, we present an immunobased microchip technology that can rapidly detect and quantify bacterial presence in various sources including physiologically relevant buffer solution (phosphate buffered saline [PBS]), blood, milk, and spinach. The microchip showed reliable capture of E. coli in PBS with an efficiency of 71.8% ± 5% at concentrations ranging from 50 to 4,000 CFUs/mL via lipopolysaccharide binding protein. The limits of detection of the microchip for PBS, blood, milk, and spinach samples were 50, 50, 50, and 500 CFUs/mL, respectively. The presented technology can be broadly applied to other pathogens at the POC, enabling various applications including surveillance of food supply and monitoring of bacteriology in patients with burn wounds. |
| first_indexed | 2024-09-23T08:09:19Z |
| format | Article |
| id | mit-1721.1/88155 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T08:09:19Z |
| publishDate | 2014 |
| publisher | Dove Medical Press |
| record_format | dspace |
| spelling | mit-1721.1/881552022-09-30T07:55:15Z Portable microfluidic chip for detection of Escherichia coli in produce and blood Wang, ShuQi Inci, Fatih Chaunzwa, Tafadzwa L. Ramanujam, Ajay Vasudevan, Aishwarya Subramanian, Sathya Ip, Alexander Chi Fai Sridharan, Banupriya Gurkan, Umut Atakan Demirci, Utkan Harvard University--MIT Division of Health Sciences and Technology Demirci, Utkan Pathogenic agents can lead to severe clinical outcomes such as food poisoning, infection of open wounds, particularly in burn injuries and sepsis. Rapid detection of these pathogens can monitor these infections in a timely manner improving clinical outcomes. Conventional bacterial detection methods, such as agar plate culture or polymerase chain reaction, are time-consuming and dependent on complex and expensive instruments, which are not suitable for point-of-care (POC) settings. Therefore, there is an unmet need to develop a simple, rapid method for detection of pathogens such as Escherichia coli. Here, we present an immunobased microchip technology that can rapidly detect and quantify bacterial presence in various sources including physiologically relevant buffer solution (phosphate buffered saline [PBS]), blood, milk, and spinach. The microchip showed reliable capture of E. coli in PBS with an efficiency of 71.8% ± 5% at concentrations ranging from 50 to 4,000 CFUs/mL via lipopolysaccharide binding protein. The limits of detection of the microchip for PBS, blood, milk, and spinach samples were 50, 50, 50, and 500 CFUs/mL, respectively. The presented technology can be broadly applied to other pathogens at the POC, enabling various applications including surveillance of food supply and monitoring of bacteriology in patients with burn wounds. United States. Army Medical Research and Materiel Command United States. Army. Telemedicine and Advanced Technology Research Center 2014-07-01T12:50:11Z 2014-07-01T12:50:11Z 2012-05 2012-01 Article http://purl.org/eprint/type/JournalArticle 1178-2013 1176-9114 http://hdl.handle.net/1721.1/88155 Wang, ShuQi, et al. “Portable Microfluidic Chip for Detection of Escherichia Coli in Produce and Blood.” International Journal of Nanomedicine (May 2012): 2591. en_US http://dx.doi.org/10.2147/ijn.s29629 International Journal of Nanomedicine Creative Commons Attribution-Noncommercial http://creativecommons.org/licenses/by-nc/3.0/ application/pdf Dove Medical Press Dove Medical Press |
| spellingShingle | Wang, ShuQi Inci, Fatih Chaunzwa, Tafadzwa L. Ramanujam, Ajay Vasudevan, Aishwarya Subramanian, Sathya Ip, Alexander Chi Fai Sridharan, Banupriya Gurkan, Umut Atakan Demirci, Utkan Portable microfluidic chip for detection of Escherichia coli in produce and blood |
| title | Portable microfluidic chip for detection of Escherichia coli in produce and blood |
| title_full | Portable microfluidic chip for detection of Escherichia coli in produce and blood |
| title_fullStr | Portable microfluidic chip for detection of Escherichia coli in produce and blood |
| title_full_unstemmed | Portable microfluidic chip for detection of Escherichia coli in produce and blood |
| title_short | Portable microfluidic chip for detection of Escherichia coli in produce and blood |
| title_sort | portable microfluidic chip for detection of escherichia coli in produce and blood |
| url | http://hdl.handle.net/1721.1/88155 |
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