Cell type–specific genetic and optogenetic tools reveal hippocampal CA2 circuits
The formation and recall of episodic memory requires precise information processing by the entorhinal-hippocampal network. For several decades, the trisynaptic circuit entorhinal cortex layer II (ECII)right arrowdentate gyrusright arrowCA3right arrowCA1 and the monosynaptic circuit ECIIIright arrowC...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2014
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| Online Access: | http://hdl.handle.net/1721.1/88164 https://orcid.org/0000-0003-2839-8228 |
| _version_ | 1811080402025054208 |
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| author | Kohara, Keigo Tonegawa, Susumu Rivest, Alexander J. Jung, Hae Yoon Kitamura, Takashi Suh, Junghyup Frank, Dominic Kajikawa, Koichiro Mise, Nathan Obata, Yuichi Wickersham, Ian R. Pignatelli di Spinazzola, Michele |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Kohara, Keigo Tonegawa, Susumu Rivest, Alexander J. Jung, Hae Yoon Kitamura, Takashi Suh, Junghyup Frank, Dominic Kajikawa, Koichiro Mise, Nathan Obata, Yuichi Wickersham, Ian R. Pignatelli di Spinazzola, Michele |
| author_sort | Kohara, Keigo |
| collection | MIT |
| description | The formation and recall of episodic memory requires precise information processing by the entorhinal-hippocampal network. For several decades, the trisynaptic circuit entorhinal cortex layer II (ECII)right arrowdentate gyrusright arrowCA3right arrowCA1 and the monosynaptic circuit ECIIIright arrowCA1 have been considered the primary substrates of the network responsible for learning and memory. Circuits linked to another hippocampal region, CA2, have only recently come to light. Using highly cell type–specific transgenic mouse lines, optogenetics and patch-clamp recordings, we found that dentate gyrus cells, long believed to not project to CA2, send functional monosynaptic inputs to CA2 pyramidal cells through abundant longitudinal projections. CA2 innervated CA1 to complete an alternate trisynaptic circuit, but, unlike CA3, projected preferentially to the deep, rather than to the superficial, sublayer of CA1. Furthermore, contrary to existing knowledge, ECIII did not project to CA2. Our results allow a deeper understanding of the biology of learning and memory. |
| first_indexed | 2024-09-23T11:31:04Z |
| format | Article |
| id | mit-1721.1/88164 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:31:04Z |
| publishDate | 2014 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/881642022-09-27T20:02:25Z Cell type–specific genetic and optogenetic tools reveal hippocampal CA2 circuits Kohara, Keigo Tonegawa, Susumu Rivest, Alexander J. Jung, Hae Yoon Kitamura, Takashi Suh, Junghyup Frank, Dominic Kajikawa, Koichiro Mise, Nathan Obata, Yuichi Wickersham, Ian R. Pignatelli di Spinazzola, Michele Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences McGovern Institute for Brain Research at MIT Picower Institute for Learning and Memory RIKEN-MIT Center for Neural Circuit Genetics Tonegawa, Susumu Tonegawa, Susumu Kohara, Keigo Pignatelli di Spinazzola, Mich Rivest, Alexander J. Jung, Hae Yoon Kitamura, Takashi Suh, Junghyup Frank, Dominic Kajikawa, Koichiro Wickersham, Ian R. The formation and recall of episodic memory requires precise information processing by the entorhinal-hippocampal network. For several decades, the trisynaptic circuit entorhinal cortex layer II (ECII)right arrowdentate gyrusright arrowCA3right arrowCA1 and the monosynaptic circuit ECIIIright arrowCA1 have been considered the primary substrates of the network responsible for learning and memory. Circuits linked to another hippocampal region, CA2, have only recently come to light. Using highly cell type–specific transgenic mouse lines, optogenetics and patch-clamp recordings, we found that dentate gyrus cells, long believed to not project to CA2, send functional monosynaptic inputs to CA2 pyramidal cells through abundant longitudinal projections. CA2 innervated CA1 to complete an alternate trisynaptic circuit, but, unlike CA3, projected preferentially to the deep, rather than to the superficial, sublayer of CA1. Furthermore, contrary to existing knowledge, ECIII did not project to CA2. Our results allow a deeper understanding of the biology of learning and memory. National Institutes of Health (U.S.) (P50-MH58880) National Institutes of Health (U.S.) (R01-MH078821) McGovern Institute for Brain Research at MIT (McGovern Institute Neurotechnology Program) Japan Society for the Promotion of Science 2014-07-01T16:10:53Z 2014-07-01T16:10:53Z 2013-12 Article http://purl.org/eprint/type/JournalArticle 1097-6256 1546-1726 http://hdl.handle.net/1721.1/88164 Kohara, Keigo, Michele Pignatelli, Alexander J Rivest, Hae-Yoon Jung, Takashi Kitamura, Junghyup Suh, Dominic Frank, et al. “Cell Type–specific Genetic and Optogenetic Tools Reveal Hippocampal CA2 Circuits.” Nat Neurosci 17, no. 2 (December 15, 2013): 269–279. https://orcid.org/0000-0003-2839-8228 en_US http://dx.doi.org/10.1038/nn.3614 Nature Neuroscience Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Nature Publishing Group Tonegawa via Courtney Crummett |
| spellingShingle | Kohara, Keigo Tonegawa, Susumu Rivest, Alexander J. Jung, Hae Yoon Kitamura, Takashi Suh, Junghyup Frank, Dominic Kajikawa, Koichiro Mise, Nathan Obata, Yuichi Wickersham, Ian R. Pignatelli di Spinazzola, Michele Cell type–specific genetic and optogenetic tools reveal hippocampal CA2 circuits |
| title | Cell type–specific genetic and optogenetic tools reveal hippocampal CA2 circuits |
| title_full | Cell type–specific genetic and optogenetic tools reveal hippocampal CA2 circuits |
| title_fullStr | Cell type–specific genetic and optogenetic tools reveal hippocampal CA2 circuits |
| title_full_unstemmed | Cell type–specific genetic and optogenetic tools reveal hippocampal CA2 circuits |
| title_short | Cell type–specific genetic and optogenetic tools reveal hippocampal CA2 circuits |
| title_sort | cell type specific genetic and optogenetic tools reveal hippocampal ca2 circuits |
| url | http://hdl.handle.net/1721.1/88164 https://orcid.org/0000-0003-2839-8228 |
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