Reproducibility of In-Vivo OCT Measured Three-Dimensional Human Lamina Cribrosa Microarchitecture
Purpose To determine the reproducibility of automated segmentation of the three-dimensional (3D) lamina cribrosa (LC) microarchitecture scanned in-vivo using optical coherence tomography (OCT). Methods Thirty-nine eyes (8 healthy, 19 glaucoma suspects and 12 glaucoma) from 49 subjects were sc...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Public Library of Science
2014
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| Online Access: | http://hdl.handle.net/1721.1/88171 https://orcid.org/0000-0001-6235-0143 https://orcid.org/0000-0002-0828-4357 |
| _version_ | 1826200489992126464 |
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| author | Wang, Bo Nevins, Jessica E. Nadler, Zach Wollstein, Gadi Ishikawa, Hiroshi Bilonick, Richard A. Kagemann, Larry Sigal, Ian A. Grulkowski, Ireneusz Liu, Jonathan Jaoshin Kraus, Martin Franz Georg Lu, Chen David Hornegger, Joachim Fujimoto, James G. Schuman, Joel S. |
| author2 | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science |
| author_facet | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Wang, Bo Nevins, Jessica E. Nadler, Zach Wollstein, Gadi Ishikawa, Hiroshi Bilonick, Richard A. Kagemann, Larry Sigal, Ian A. Grulkowski, Ireneusz Liu, Jonathan Jaoshin Kraus, Martin Franz Georg Lu, Chen David Hornegger, Joachim Fujimoto, James G. Schuman, Joel S. |
| author_sort | Wang, Bo |
| collection | MIT |
| description | Purpose
To determine the reproducibility of automated segmentation of the three-dimensional (3D) lamina cribrosa (LC) microarchitecture scanned in-vivo using optical coherence tomography (OCT).
Methods
Thirty-nine eyes (8 healthy, 19 glaucoma suspects and 12 glaucoma) from 49 subjects were scanned twice using swept-source (SS−) OCT in a 3.5×3.5×3.64 mm (400×400×896 pixels) volume centered on the optic nerve head, with the focus readjusted after each scan. The LC was automatically segmented and analyzed for microarchitectural parameters, including pore diameter, pore diameter standard deviation (SD), pore aspect ratio, pore area, beam thickness, beam thickness SD, and beam thickness to pore diameter ratio. Reproducibility of the parameters was assessed by computing the imprecision of the parameters between the scans.
Results
The automated segmentation demonstrated excellent reproducibility. All LC microarchitecture parameters had an imprecision of less or equal to 4.2%. There was little variability in imprecision with respect to diagnostic category, although the method tends to show higher imprecision amongst healthy subjects.
Conclusion
The proposed automated segmentation of the LC demonstrated high reproducibility for 3D LC parameters. This segmentation analysis tool will be useful for in-vivo studies of the LC. |
| first_indexed | 2024-09-23T11:37:16Z |
| format | Article |
| id | mit-1721.1/88171 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:37:16Z |
| publishDate | 2014 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/881712022-09-27T20:45:44Z Reproducibility of In-Vivo OCT Measured Three-Dimensional Human Lamina Cribrosa Microarchitecture Wang, Bo Nevins, Jessica E. Nadler, Zach Wollstein, Gadi Ishikawa, Hiroshi Bilonick, Richard A. Kagemann, Larry Sigal, Ian A. Grulkowski, Ireneusz Liu, Jonathan Jaoshin Kraus, Martin Franz Georg Lu, Chen David Hornegger, Joachim Fujimoto, James G. Schuman, Joel S. Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Grulkowski, Ireneusz Liu, Jonathan Jaoshin Kraus, Martin Franz Georg Lu, Chen David Fujimoto, James G. Purpose To determine the reproducibility of automated segmentation of the three-dimensional (3D) lamina cribrosa (LC) microarchitecture scanned in-vivo using optical coherence tomography (OCT). Methods Thirty-nine eyes (8 healthy, 19 glaucoma suspects and 12 glaucoma) from 49 subjects were scanned twice using swept-source (SS−) OCT in a 3.5×3.5×3.64 mm (400×400×896 pixels) volume centered on the optic nerve head, with the focus readjusted after each scan. The LC was automatically segmented and analyzed for microarchitectural parameters, including pore diameter, pore diameter standard deviation (SD), pore aspect ratio, pore area, beam thickness, beam thickness SD, and beam thickness to pore diameter ratio. Reproducibility of the parameters was assessed by computing the imprecision of the parameters between the scans. Results The automated segmentation demonstrated excellent reproducibility. All LC microarchitecture parameters had an imprecision of less or equal to 4.2%. There was little variability in imprecision with respect to diagnostic category, although the method tends to show higher imprecision amongst healthy subjects. Conclusion The proposed automated segmentation of the LC demonstrated high reproducibility for 3D LC parameters. This segmentation analysis tool will be useful for in-vivo studies of the LC. Eye & Ear Foundation of Pittsburgh Research to Prevent Blindness, Inc. (United States) National Institutes of Health (U.S.) (contract R01-EY013178) National Institutes of Health (U.S.) (contract R01-EY011289) National Institutes of Health (U.S.) (contract P30-EY008098) 2014-07-01T19:19:39Z 2014-07-01T19:19:39Z 2014-04 2013-12 Article http://purl.org/eprint/type/JournalArticle 1932-6203 http://hdl.handle.net/1721.1/88171 Wang, Bo, Jessica E. Nevins, Zach Nadler, Gadi Wollstein, Hiroshi Ishikawa, Richard A. Bilonick, Larry Kagemann, et al. “Reproducibility of In-Vivo OCT Measured Three-Dimensional Human Lamina Cribrosa Microarchitecture.” Edited by Knut Stieger. PLoS ONE 9, no. 4 (April 18, 2014): e95526. https://orcid.org/0000-0001-6235-0143 https://orcid.org/0000-0002-0828-4357 en_US http://dx.doi.org/10.1371/journal.pone.0095526 PLoS ONE Creative Commons Attribution http://creativecommons.org/licenses/by/4.0/ application/pdf Public Library of Science Public Library of Science |
| spellingShingle | Wang, Bo Nevins, Jessica E. Nadler, Zach Wollstein, Gadi Ishikawa, Hiroshi Bilonick, Richard A. Kagemann, Larry Sigal, Ian A. Grulkowski, Ireneusz Liu, Jonathan Jaoshin Kraus, Martin Franz Georg Lu, Chen David Hornegger, Joachim Fujimoto, James G. Schuman, Joel S. Reproducibility of In-Vivo OCT Measured Three-Dimensional Human Lamina Cribrosa Microarchitecture |
| title | Reproducibility of In-Vivo OCT Measured Three-Dimensional Human Lamina Cribrosa Microarchitecture |
| title_full | Reproducibility of In-Vivo OCT Measured Three-Dimensional Human Lamina Cribrosa Microarchitecture |
| title_fullStr | Reproducibility of In-Vivo OCT Measured Three-Dimensional Human Lamina Cribrosa Microarchitecture |
| title_full_unstemmed | Reproducibility of In-Vivo OCT Measured Three-Dimensional Human Lamina Cribrosa Microarchitecture |
| title_short | Reproducibility of In-Vivo OCT Measured Three-Dimensional Human Lamina Cribrosa Microarchitecture |
| title_sort | reproducibility of in vivo oct measured three dimensional human lamina cribrosa microarchitecture |
| url | http://hdl.handle.net/1721.1/88171 https://orcid.org/0000-0001-6235-0143 https://orcid.org/0000-0002-0828-4357 |
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