Comprehensive Analysis of Human Cells Motion under an Irrotational AC Electric Field in an Electro-Microfluidic Chip
AC electrokinetics is a versatile tool for contact-less manipulation or characterization of cells and has been widely used for separation based on genotype translation to electrical phenotypes. Cells responses to an AC electric field result in a complex combination of electrokinetic phenomena, mainl...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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Public Library of Science
2014
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| Online Access: | http://hdl.handle.net/1721.1/88193 |
| _version_ | 1811090815041142784 |
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| author | Vaillier, Clarisse Honegger, Thibault Kermarrec, Frederique Gidrol, Xavier Peyrade, David |
| author2 | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science |
| author_facet | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Vaillier, Clarisse Honegger, Thibault Kermarrec, Frederique Gidrol, Xavier Peyrade, David |
| author_sort | Vaillier, Clarisse |
| collection | MIT |
| description | AC electrokinetics is a versatile tool for contact-less manipulation or characterization of cells and has been widely used for separation based on genotype translation to electrical phenotypes. Cells responses to an AC electric field result in a complex combination of electrokinetic phenomena, mainly dielectrophoresis and electrohydrodynamic forces. Human cells behaviors to AC electrokinetics remain unclear over a large frequency spectrum as illustrated by the self-rotation effect observed recently. We here report and analyze human cells behaviors in different conditions of medium conductivity, electric field frequency and magnitude. We also observe the self-rotation of human cells, in the absence of a rotational electric field. Based on an analytical competitive model of electrokinetic forces, we propose an explanation of the cell self-rotation. These experimental results, coupled with our model, lead to the exploitation of the cell behaviors to measure the intrinsic dielectric properties of JURKAT, HEK and PC3 human cell lines. |
| first_indexed | 2024-09-23T14:52:23Z |
| format | Article |
| id | mit-1721.1/88193 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:52:23Z |
| publishDate | 2014 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/881932022-10-01T23:02:59Z Comprehensive Analysis of Human Cells Motion under an Irrotational AC Electric Field in an Electro-Microfluidic Chip Vaillier, Clarisse Honegger, Thibault Kermarrec, Frederique Gidrol, Xavier Peyrade, David Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Honegger, Thibault AC electrokinetics is a versatile tool for contact-less manipulation or characterization of cells and has been widely used for separation based on genotype translation to electrical phenotypes. Cells responses to an AC electric field result in a complex combination of electrokinetic phenomena, mainly dielectrophoresis and electrohydrodynamic forces. Human cells behaviors to AC electrokinetics remain unclear over a large frequency spectrum as illustrated by the self-rotation effect observed recently. We here report and analyze human cells behaviors in different conditions of medium conductivity, electric field frequency and magnitude. We also observe the self-rotation of human cells, in the absence of a rotational electric field. Based on an analytical competitive model of electrokinetic forces, we propose an explanation of the cell self-rotation. These experimental results, coupled with our model, lead to the exploitation of the cell behaviors to measure the intrinsic dielectric properties of JURKAT, HEK and PC3 human cell lines. 2014-07-08T16:46:46Z 2014-07-08T16:46:46Z 2014-04 2013-11 Article http://purl.org/eprint/type/JournalArticle 1932-6203 http://hdl.handle.net/1721.1/88193 Vaillier, Clarisse, Thibault Honegger, Frederique Kermarrec, Xavier Gidrol, and David Peyrade. “Comprehensive Analysis of Human Cells Motion Under an Irrotational AC Electric Field in an Electro-Microfluidic Chip.” Edited by Aristides Docoslis. PLoS ONE 9, no. 4 (April 15, 2014): e95231. en_US http://dx.doi.org/10.1371/journal.pone.0095231 PLoS ONE Creative Commons Attribution http://creativecommons.org/licenses/by/4.0/ application/pdf Public Library of Science Public Library of Science |
| spellingShingle | Vaillier, Clarisse Honegger, Thibault Kermarrec, Frederique Gidrol, Xavier Peyrade, David Comprehensive Analysis of Human Cells Motion under an Irrotational AC Electric Field in an Electro-Microfluidic Chip |
| title | Comprehensive Analysis of Human Cells Motion under an Irrotational AC Electric Field in an Electro-Microfluidic Chip |
| title_full | Comprehensive Analysis of Human Cells Motion under an Irrotational AC Electric Field in an Electro-Microfluidic Chip |
| title_fullStr | Comprehensive Analysis of Human Cells Motion under an Irrotational AC Electric Field in an Electro-Microfluidic Chip |
| title_full_unstemmed | Comprehensive Analysis of Human Cells Motion under an Irrotational AC Electric Field in an Electro-Microfluidic Chip |
| title_short | Comprehensive Analysis of Human Cells Motion under an Irrotational AC Electric Field in an Electro-Microfluidic Chip |
| title_sort | comprehensive analysis of human cells motion under an irrotational ac electric field in an electro microfluidic chip |
| url | http://hdl.handle.net/1721.1/88193 |
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