Intersectional Cre Driver Lines Generated Using Split-Intein Mediated Split-Cre Reconstitution
Tissue and cell type highly specific Cre drivers are very rare due to the fact that most genes or promoters used to direct Cre expressions are generally expressed in more than one tissues and/or in multiple cell types. We developed a split-intein based split-Cre system for highly efficient Cre-recon...
| Main Authors: | , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Nature Publishing Group
2014
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| Online Access: | http://hdl.handle.net/1721.1/88237 https://orcid.org/0000-0002-8021-277X |
| _version_ | 1811084051560267776 |
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| author | Wang, Ping Chen, Tianrui Sakurai, Katsuyasu Han, Bao-Xia He, Zhigang Feng, Guoping Wang, Fan |
| author2 | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences |
| author_facet | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Wang, Ping Chen, Tianrui Sakurai, Katsuyasu Han, Bao-Xia He, Zhigang Feng, Guoping Wang, Fan |
| author_sort | Wang, Ping |
| collection | MIT |
| description | Tissue and cell type highly specific Cre drivers are very rare due to the fact that most genes or promoters used to direct Cre expressions are generally expressed in more than one tissues and/or in multiple cell types. We developed a split-intein based split-Cre system for highly efficient Cre-reconstitution through protein splicing. This split-intein-split-Cre system can be used to intersect the expression patterns of two genes or promoters to restrict full-length Cre reconstitution in their overlapping domains. To test this system in vivo, we selected several conserved human enhancers to drive the expression of either Cre-N-intein-N, or intein-C-Cre-C transgene in different brain regions. In all paired CreN/CreC transgenic mice, Cre-dependent reporter was efficiently induced specifically in the intersectional expression domains of two enhancers. This split-intein based method is simpler to implement compared with other strategies for generating highly-restricted intersectional Cre drivers to study complex tissues such as the nervous system. |
| first_indexed | 2024-09-23T12:43:54Z |
| format | Article |
| id | mit-1721.1/88237 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T12:43:54Z |
| publishDate | 2014 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/882372022-09-28T09:41:12Z Intersectional Cre Driver Lines Generated Using Split-Intein Mediated Split-Cre Reconstitution Wang, Ping Chen, Tianrui Sakurai, Katsuyasu Han, Bao-Xia He, Zhigang Feng, Guoping Wang, Fan Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences McGovern Institute for Brain Research at MIT Feng, Guoping Tissue and cell type highly specific Cre drivers are very rare due to the fact that most genes or promoters used to direct Cre expressions are generally expressed in more than one tissues and/or in multiple cell types. We developed a split-intein based split-Cre system for highly efficient Cre-reconstitution through protein splicing. This split-intein-split-Cre system can be used to intersect the expression patterns of two genes or promoters to restrict full-length Cre reconstitution in their overlapping domains. To test this system in vivo, we selected several conserved human enhancers to drive the expression of either Cre-N-intein-N, or intein-C-Cre-C transgene in different brain regions. In all paired CreN/CreC transgenic mice, Cre-dependent reporter was efficiently induced specifically in the intersectional expression domains of two enhancers. This split-intein based method is simpler to implement compared with other strategies for generating highly-restricted intersectional Cre drivers to study complex tissues such as the nervous system. National Institutes of Health (U.S.) (NIH RC1NS068612) National Institutes of Health (U.S.) (DA028302) National Institutes of Health (U.S.) (R01 MH081201) Simons Foundation (Autism Research Initiative) 2014-07-09T16:40:44Z 2014-07-09T16:40:44Z 2012-07 2012-05 Article http://purl.org/eprint/type/JournalArticle 2045-2322 http://hdl.handle.net/1721.1/88237 Wang, Ping, Tianrui Chen, Katsuyasu Sakurai, Bao-Xia Han, Zhigang He, Guoping Feng, and Fan Wang. “Intersectional Cre Driver Lines Generated Using Split-Intein Mediated Split-Cre Reconstitution.” Sci. Rep. 2 (July 6, 2012). https://orcid.org/0000-0002-8021-277X en_US http://dx.doi.org/10.1038/srep00497 Scientific Reports Creative Commons Attribution-NonCommercial-ShareAlike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Nature Publishing Group Nature Publishing Group |
| spellingShingle | Wang, Ping Chen, Tianrui Sakurai, Katsuyasu Han, Bao-Xia He, Zhigang Feng, Guoping Wang, Fan Intersectional Cre Driver Lines Generated Using Split-Intein Mediated Split-Cre Reconstitution |
| title | Intersectional Cre Driver Lines Generated Using Split-Intein Mediated Split-Cre Reconstitution |
| title_full | Intersectional Cre Driver Lines Generated Using Split-Intein Mediated Split-Cre Reconstitution |
| title_fullStr | Intersectional Cre Driver Lines Generated Using Split-Intein Mediated Split-Cre Reconstitution |
| title_full_unstemmed | Intersectional Cre Driver Lines Generated Using Split-Intein Mediated Split-Cre Reconstitution |
| title_short | Intersectional Cre Driver Lines Generated Using Split-Intein Mediated Split-Cre Reconstitution |
| title_sort | intersectional cre driver lines generated using split intein mediated split cre reconstitution |
| url | http://hdl.handle.net/1721.1/88237 https://orcid.org/0000-0002-8021-277X |
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