Multicellular self-assembly on patterned surfaces
Thesis (Ph.D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 2000.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2005
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| Online Access: | http://hdl.handle.net/1721.1/8826 |
| _version_ | 1826192238330249216 |
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| author | Fujii, Jennifer T. (Jennifer Tomiko), 1972- |
| author2 | Linda G. Griffith. |
| author_facet | Linda G. Griffith. Fujii, Jennifer T. (Jennifer Tomiko), 1972- |
| author_sort | Fujii, Jennifer T. (Jennifer Tomiko), 1972- |
| collection | MIT |
| description | Thesis (Ph.D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 2000. |
| first_indexed | 2024-09-23T09:08:08Z |
| format | Thesis |
| id | mit-1721.1/8826 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T09:08:08Z |
| publishDate | 2005 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/88262019-04-10T20:56:57Z Multicellular self-assembly on patterned surfaces Fujii, Jennifer T. (Jennifer Tomiko), 1972- Linda G. Griffith. Massachusetts Institute of Technology. Dept. of Chemical Engineering. Massachusetts Institute of Technology. Dept. of Chemical Engineering. Chemical Engineering. Thesis (Ph.D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 2000. Includes bibliographical references. Controlling the spatial distribution of cells in two and three dimensions may be important in the design of advanced tissue engineering scaffolds and other biomedical applications. In this thesis, the concept of biophysical sorting was applied as a method to control the spatial distribution of cells. This approach relies on a self-assembly process that is dependent, in part, on the intrinsic adhesivity of cells. A model system was developed using a simple patterning technique to prepare surfaces with alternating regions that supported variable cell response. First, the influence of certain biophysical parameters that may govern multicellular assembly of a single cell type on patterned surfaces was quantitatively investigated. For surfaces patterned with small features that allow cells to sample surrounding regions through membrane protrusions, it was found that a dynamic equilibrium distribution of cells correlated with differen~es in cell-substratum adhesion strength. The approach to that distribution, however, could be kinetically limited by the inability of the individual cells to sample adjacent areas of the patterned surface. This kinetic limitation was studied on surfaces with increasingly large feature sizes, and found that a simple diffusion model of migration may not completely describe the present system. Other effects such as contact inhibited motility and an induction time for migration may also influence multicellular assembly. The potential of multicellular assembly to simultaneously control the distribution of two cell types was also investigated. First, the multicellular assembly of each cell type was studied in isolation. Co-culture experiments indicated that, in addition to the factors that govern the assembly of a single cell type, sorting of two cell types depended on cell density. Images of high cell density co-cultures suggest that incomplete biophysical separation was achieved. by Jennifer T. Fujii. Ph.D. 2005-08-23T15:38:48Z 2005-08-23T15:38:48Z 2000 2000 Thesis http://hdl.handle.net/1721.1/8826 48385721 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 173 leaves 12033234 bytes 12032988 bytes application/pdf application/pdf application/pdf Massachusetts Institute of Technology |
| spellingShingle | Chemical Engineering. Fujii, Jennifer T. (Jennifer Tomiko), 1972- Multicellular self-assembly on patterned surfaces |
| title | Multicellular self-assembly on patterned surfaces |
| title_full | Multicellular self-assembly on patterned surfaces |
| title_fullStr | Multicellular self-assembly on patterned surfaces |
| title_full_unstemmed | Multicellular self-assembly on patterned surfaces |
| title_short | Multicellular self-assembly on patterned surfaces |
| title_sort | multicellular self assembly on patterned surfaces |
| topic | Chemical Engineering. |
| url | http://hdl.handle.net/1721.1/8826 |
| work_keys_str_mv | AT fujiijennifertjennifertomiko1972 multicellularselfassemblyonpatternedsurfaces |