Ronin/Hcf-1 binds to a hyperconserved enhancer element and regulates genes involved in the growth of embryonic stem cells
Self-renewing embryonic stem (ES) cells have an exceptional need for timely biomass production, yet the transcriptional control mechanisms responsible for meeting this requirement are largely unknown. We report here that Ronin (Thap11), which is essential for the self-renewal of ES cells, binds with...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Cold Spring Harbor Laboratory Press
2014
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| Online Access: | http://hdl.handle.net/1721.1/88437 https://orcid.org/0000-0001-8855-8647 |
| _version_ | 1826213997162004480 |
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| author | Dejosez, M. Frampton, Garrett M. Whyte, W. A. Stratton, S. A. Barton, Michelle C. Gunaratne, P. H. Zwaka, T. P. Levine, Stuart S. Young, Richard A. |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Dejosez, M. Frampton, Garrett M. Whyte, W. A. Stratton, S. A. Barton, Michelle C. Gunaratne, P. H. Zwaka, T. P. Levine, Stuart S. Young, Richard A. |
| author_sort | Dejosez, M. |
| collection | MIT |
| description | Self-renewing embryonic stem (ES) cells have an exceptional need for timely biomass production, yet the transcriptional control mechanisms responsible for meeting this requirement are largely unknown. We report here that Ronin (Thap11), which is essential for the self-renewal of ES cells, binds with its transcriptional coregulator, Hcf-1, to a highly conserved enhancer element that previously lacked a recognized binding factor. The subset of genes bound by Ronin/Hcf-1 function primarily in transcription initiation, mRNA splicing, and cell metabolism; genes involved in cell signaling and cell development are conspicuously underrepresented in this target gene repertoire. Although Ronin/Hcf-1 represses the expression of some target genes, its activity at promoter sites more often leads to the up-regulation of genes essential to protein biosynthesis and energy production. We propose that Ronin/Hcf-1 controls a genetic program that contributes to the unimpeded growth of ES cells. |
| first_indexed | 2024-09-23T15:58:10Z |
| format | Article |
| id | mit-1721.1/88437 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:58:10Z |
| publishDate | 2014 |
| publisher | Cold Spring Harbor Laboratory Press |
| record_format | dspace |
| spelling | mit-1721.1/884372022-09-29T17:21:51Z Ronin/Hcf-1 binds to a hyperconserved enhancer element and regulates genes involved in the growth of embryonic stem cells Dejosez, M. Frampton, Garrett M. Whyte, W. A. Stratton, S. A. Barton, Michelle C. Gunaratne, P. H. Zwaka, T. P. Levine, Stuart S. Young, Richard A. Massachusetts Institute of Technology. Department of Biology Whitehead Institute for Biomedical Research Levine, Stuart S. Young, Richard A. Self-renewing embryonic stem (ES) cells have an exceptional need for timely biomass production, yet the transcriptional control mechanisms responsible for meeting this requirement are largely unknown. We report here that Ronin (Thap11), which is essential for the self-renewal of ES cells, binds with its transcriptional coregulator, Hcf-1, to a highly conserved enhancer element that previously lacked a recognized binding factor. The subset of genes bound by Ronin/Hcf-1 function primarily in transcription initiation, mRNA splicing, and cell metabolism; genes involved in cell signaling and cell development are conspicuously underrepresented in this target gene repertoire. Although Ronin/Hcf-1 represses the expression of some target genes, its activity at promoter sites more often leads to the up-regulation of genes essential to protein biosynthesis and energy production. We propose that Ronin/Hcf-1 controls a genetic program that contributes to the unimpeded growth of ES cells. National Institutes of Health (U.S.) (Grant R01 EB005173-01) National Institutes of Health (U.S.) (Grant 1R01 GM077442-01) National Institutes of Health (U.S.) (Grant P20 EB007076) National Institutes of Health (U.S.) (Grant P01 GM81627) National Institutes of Health (U.S.) (Grant 2R01 HG002668) 2014-07-18T14:26:22Z 2014-07-18T14:26:22Z 2010-06 2010-05 Article http://purl.org/eprint/type/JournalArticle 0890-9369 1549-5477 http://hdl.handle.net/1721.1/88437 Dejosez, M., S. S. Levine, G. M. Frampton, W. A. Whyte, S. A. Stratton, M. C. Barton, P. H. Gunaratne, R. A. Young, and T. P. Zwaka. “Ronin/Hcf-1 Binds to a Hyperconserved Enhancer Element and Regulates Genes Involved in the Growth of Embryonic Stem Cells.” Genes & Development 24, no. 14 (July 15, 2010): 1479–1484. © 2010 by Cold Spring Harbor Laboratory Press https://orcid.org/0000-0001-8855-8647 en_US http://dx.doi.org/10.1101/gad.1935210 Genes & Development Article is available under a Creative Commons license; see publisher’s site for details. http://creativecommons.org/ application/pdf Cold Spring Harbor Laboratory Press Cold Spring Harbor Laboratory Press |
| spellingShingle | Dejosez, M. Frampton, Garrett M. Whyte, W. A. Stratton, S. A. Barton, Michelle C. Gunaratne, P. H. Zwaka, T. P. Levine, Stuart S. Young, Richard A. Ronin/Hcf-1 binds to a hyperconserved enhancer element and regulates genes involved in the growth of embryonic stem cells |
| title | Ronin/Hcf-1 binds to a hyperconserved enhancer element and regulates genes involved in the growth of embryonic stem cells |
| title_full | Ronin/Hcf-1 binds to a hyperconserved enhancer element and regulates genes involved in the growth of embryonic stem cells |
| title_fullStr | Ronin/Hcf-1 binds to a hyperconserved enhancer element and regulates genes involved in the growth of embryonic stem cells |
| title_full_unstemmed | Ronin/Hcf-1 binds to a hyperconserved enhancer element and regulates genes involved in the growth of embryonic stem cells |
| title_short | Ronin/Hcf-1 binds to a hyperconserved enhancer element and regulates genes involved in the growth of embryonic stem cells |
| title_sort | ronin hcf 1 binds to a hyperconserved enhancer element and regulates genes involved in the growth of embryonic stem cells |
| url | http://hdl.handle.net/1721.1/88437 https://orcid.org/0000-0001-8855-8647 |
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