Direct observation of mammalian cell growth and size regulation
We introduce a microfluidic system for simultaneously measuring single-cell mass and cell cycle progression over multiple generations. We use this system to obtain over 1,000 h of growth data from mouse lymphoblast and pro–B-cell lymphoid cell lines. Cell lineage analysis revealed a decrease in the...
| Main Authors: | , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Nature Publishing Group
2014
|
| Online Access: | http://hdl.handle.net/1721.1/88951 https://orcid.org/0000-0001-5223-9433 |
| _version_ | 1811088433284644864 |
|---|---|
| author | Son, Sungmin Tzur, Amit Weng, Yaochung Jorgensen, Paul Kim, Jisoo Kirschner, Marc W. Manalis, Scott R |
| author2 | Massachusetts Institute of Technology. Computational and Systems Biology Program |
| author_facet | Massachusetts Institute of Technology. Computational and Systems Biology Program Son, Sungmin Tzur, Amit Weng, Yaochung Jorgensen, Paul Kim, Jisoo Kirschner, Marc W. Manalis, Scott R |
| author_sort | Son, Sungmin |
| collection | MIT |
| description | We introduce a microfluidic system for simultaneously measuring single-cell mass and cell cycle progression over multiple generations. We use this system to obtain over 1,000 h of growth data from mouse lymphoblast and pro–B-cell lymphoid cell lines. Cell lineage analysis revealed a decrease in the growth rate variability at the G1-S phase transition, which suggests the presence of a growth rate threshold for maintaining size homeostasis. |
| first_indexed | 2024-09-23T14:02:07Z |
| format | Article |
| id | mit-1721.1/88951 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:02:07Z |
| publishDate | 2014 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/889512022-09-28T17:49:13Z Direct observation of mammalian cell growth and size regulation Son, Sungmin Tzur, Amit Weng, Yaochung Jorgensen, Paul Kim, Jisoo Kirschner, Marc W. Manalis, Scott R Massachusetts Institute of Technology. Computational and Systems Biology Program Massachusetts Institute of Technology. Department of Mechanical Engineering Koch Institute for Integrative Cancer Research at MIT Son, Sungmin Weng, Yaochung Kim, Jisoo Manalis, Scott R. We introduce a microfluidic system for simultaneously measuring single-cell mass and cell cycle progression over multiple generations. We use this system to obtain over 1,000 h of growth data from mouse lymphoblast and pro–B-cell lymphoid cell lines. Cell lineage analysis revealed a decrease in the growth rate variability at the G1-S phase transition, which suggests the presence of a growth rate threshold for maintaining size homeostasis. National Cancer Institute (U.S.) (Physical Sciences Oncology Center, U54CA143874) National Cancer Institute (U.S.) (Physical Sciences Oncology Center, R21 CA137695) National Institute of General Medical Sciences (U.S.) ((NIGMS) EUREKA R01GM085457) Kwanjeong Educational Foundation (Korea) (Graduate Fellowship) National Science Foundation (U.S.) (Graduate Research Fellowship) European Commission. Community Research and Development Information Service (Marie Curie International Reintegration Grant PIRG-GA-2010-277062) Israeli Centers of Research Excellence (I-CORE) (Program, Center no. 41/11) 2014-08-21T15:47:03Z 2014-08-21T15:47:03Z 2012-08 2012-05 Article http://purl.org/eprint/type/JournalArticle 1548-7091 1548-7105 http://hdl.handle.net/1721.1/88951 Son, Sungmin, Amit Tzur, Yaochung Weng, Paul Jorgensen, Jisoo Kim, Marc W Kirschner, and Scott R Manalis. “Direct Observation of Mammalian Cell Growth and Size Regulation.” Nature Methods 9, no. 9 (August 5, 2012): 910–912. https://orcid.org/0000-0001-5223-9433 en_US http://dx.doi.org/10.1038/nmeth.2133 Nature Methods Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Nature Publishing Group PMC |
| spellingShingle | Son, Sungmin Tzur, Amit Weng, Yaochung Jorgensen, Paul Kim, Jisoo Kirschner, Marc W. Manalis, Scott R Direct observation of mammalian cell growth and size regulation |
| title | Direct observation of mammalian cell growth and size regulation |
| title_full | Direct observation of mammalian cell growth and size regulation |
| title_fullStr | Direct observation of mammalian cell growth and size regulation |
| title_full_unstemmed | Direct observation of mammalian cell growth and size regulation |
| title_short | Direct observation of mammalian cell growth and size regulation |
| title_sort | direct observation of mammalian cell growth and size regulation |
| url | http://hdl.handle.net/1721.1/88951 https://orcid.org/0000-0001-5223-9433 |
| work_keys_str_mv | AT sonsungmin directobservationofmammaliancellgrowthandsizeregulation AT tzuramit directobservationofmammaliancellgrowthandsizeregulation AT wengyaochung directobservationofmammaliancellgrowthandsizeregulation AT jorgensenpaul directobservationofmammaliancellgrowthandsizeregulation AT kimjisoo directobservationofmammaliancellgrowthandsizeregulation AT kirschnermarcw directobservationofmammaliancellgrowthandsizeregulation AT manalisscottr directobservationofmammaliancellgrowthandsizeregulation |