Microfluidic genome-wide profiling of intrinsic electrical properties in Saccharomyces cerevisiae
Methods to analyze the intrinsic physical properties of cells – for example, size, density, rigidity, or electrical properties – are an active area of interest in the microfluidics community. Although the physical properties of cells are determined at a fundamental level by gene expression, the rela...
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| 格式: | Article |
| 語言: | en_US |
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Royal Society of Chemistry
2014
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| 在線閱讀: | http://hdl.handle.net/1721.1/88978 https://orcid.org/0000-0001-8898-2296 https://orcid.org/0000-0002-7112-1454 |
| _version_ | 1826212726423158784 |
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| author | Vahey, Michael D. Quiros Pesudo, Laia Svensson, J. Peter Voldman, Joel Samson, Leona D |
| author2 | Massachusetts Institute of Technology. Center for Environmental Health Sciences |
| author_facet | Massachusetts Institute of Technology. Center for Environmental Health Sciences Vahey, Michael D. Quiros Pesudo, Laia Svensson, J. Peter Voldman, Joel Samson, Leona D |
| author_sort | Vahey, Michael D. |
| collection | MIT |
| description | Methods to analyze the intrinsic physical properties of cells – for example, size, density, rigidity, or electrical properties – are an active area of interest in the microfluidics community. Although the physical properties of cells are determined at a fundamental level by gene expression, the relationship between the two remains exceptionally complex and poorly characterized, limiting the adoption of intrinsic separation technologies. To improve our current understanding of how a cell's genotype maps to a measurable physical characteristic and quantitatively investigate the potential of using these characteristics as biomarkers, we have developed a novel screen that combines microfluidic cell sorting with high-throughput sequencing and the haploid yeast deletion library to identify genes whose functions modulate one such characteristic – intrinsic electrical properties. Using this screen, we are able to establish a high-content electrical profile of the haploid yeast gene deletion strains. We find that individual genetic deletions can appreciably alter the electrical properties of cells, affecting [approximately] 10% of the 4432 gene deletion strains screened. Additionally, we find that gene deletions affecting electrical properties in specific ways (i.e. increasing or decreasing effective conductivity at higher or lower electric field frequencies) are strongly associated with an enriched subset of fundamental biological processes that can be traced to specific pathways and complexes. The screening approach demonstrated here and the attendant results are immediately applicable to the intrinsic separations community. |
| first_indexed | 2024-09-23T15:37:11Z |
| format | Article |
| id | mit-1721.1/88978 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:37:11Z |
| publishDate | 2014 |
| publisher | Royal Society of Chemistry |
| record_format | dspace |
| spelling | mit-1721.1/889782022-09-29T15:00:05Z Microfluidic genome-wide profiling of intrinsic electrical properties in Saccharomyces cerevisiae Vahey, Michael D. Quiros Pesudo, Laia Svensson, J. Peter Voldman, Joel Samson, Leona D Massachusetts Institute of Technology. Center for Environmental Health Sciences Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Koch Institute for Integrative Cancer Research at MIT Vahey, Michael D. Quiros Pesudo, Laia Samson, Leona D. Voldman, Joel Methods to analyze the intrinsic physical properties of cells – for example, size, density, rigidity, or electrical properties – are an active area of interest in the microfluidics community. Although the physical properties of cells are determined at a fundamental level by gene expression, the relationship between the two remains exceptionally complex and poorly characterized, limiting the adoption of intrinsic separation technologies. To improve our current understanding of how a cell's genotype maps to a measurable physical characteristic and quantitatively investigate the potential of using these characteristics as biomarkers, we have developed a novel screen that combines microfluidic cell sorting with high-throughput sequencing and the haploid yeast deletion library to identify genes whose functions modulate one such characteristic – intrinsic electrical properties. Using this screen, we are able to establish a high-content electrical profile of the haploid yeast gene deletion strains. We find that individual genetic deletions can appreciably alter the electrical properties of cells, affecting [approximately] 10% of the 4432 gene deletion strains screened. Additionally, we find that gene deletions affecting electrical properties in specific ways (i.e. increasing or decreasing effective conductivity at higher or lower electric field frequencies) are strongly associated with an enriched subset of fundamental biological processes that can be traced to specific pathways and complexes. The screening approach demonstrated here and the attendant results are immediately applicable to the intrinsic separations community. Singapore-MIT Alliance National Science Foundation (U.S.) (NSF IDBR grant DBI-0852654) National Institutes of Health (U.S.) (NIH grant EB005753) 2014-08-22T15:42:11Z 2014-08-22T15:42:11Z 2013-04 2013-02 Article http://purl.org/eprint/type/JournalArticle 1473-0197 1473-0189 http://hdl.handle.net/1721.1/88978 Vahey, Michael D., Laia Quiros Pesudo, J. Peter Svensson, Leona D. Samson, and Joel Voldman. “Microfluidic Genome-Wide Profiling of Intrinsic Electrical Properties in Saccharomyces Cerevisiae.” Lab Chip 13, no. 14 (2013): 2754. https://orcid.org/0000-0001-8898-2296 https://orcid.org/0000-0002-7112-1454 en_US http://dx.doi.org/10.1039/c3lc50162k Lab on a Chip Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Royal Society of Chemistry PMC |
| spellingShingle | Vahey, Michael D. Quiros Pesudo, Laia Svensson, J. Peter Voldman, Joel Samson, Leona D Microfluidic genome-wide profiling of intrinsic electrical properties in Saccharomyces cerevisiae |
| title | Microfluidic genome-wide profiling of intrinsic electrical properties in Saccharomyces cerevisiae |
| title_full | Microfluidic genome-wide profiling of intrinsic electrical properties in Saccharomyces cerevisiae |
| title_fullStr | Microfluidic genome-wide profiling of intrinsic electrical properties in Saccharomyces cerevisiae |
| title_full_unstemmed | Microfluidic genome-wide profiling of intrinsic electrical properties in Saccharomyces cerevisiae |
| title_short | Microfluidic genome-wide profiling of intrinsic electrical properties in Saccharomyces cerevisiae |
| title_sort | microfluidic genome wide profiling of intrinsic electrical properties in saccharomyces cerevisiae |
| url | http://hdl.handle.net/1721.1/88978 https://orcid.org/0000-0001-8898-2296 https://orcid.org/0000-0002-7112-1454 |
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