Quantifying Particle Coatings Using High-Precision Mass Measurements
We present a general method to quantify coatings on microparticle surfaces based on the additional mass. Particle buoyant mass is determined in a solution with a density that is nearly equivalent to that of the core particle, reducing the magnitude and uncertainty of the measurement. Under these con...
| Main Authors: | , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
American Chemical Society
2014
|
| Online Access: | http://hdl.handle.net/1721.1/88985 https://orcid.org/0000-0001-5223-9433 |
| _version_ | 1826211939610525696 |
|---|---|
| author | Knudsen, Scott Michael von Muhlen, Marcio G. Manalis, Scott R. |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Knudsen, Scott Michael von Muhlen, Marcio G. Manalis, Scott R. |
| author_sort | Knudsen, Scott Michael |
| collection | MIT |
| description | We present a general method to quantify coatings on microparticle surfaces based on the additional mass. Particle buoyant mass is determined in a solution with a density that is nearly equivalent to that of the core particle, reducing the magnitude and uncertainty of the measurement. Under these conditions, added material with a different density than that of the core is a larger fraction of the total buoyant mass of the coated particle. This method can resolve a buoyant mass difference between uncoated and coated particles of 1 fg. For the protein layer on the 3 μm polystyrene spheres measured herein, this is equivalent to 1/10th of a full layer. |
| first_indexed | 2024-09-23T15:13:31Z |
| format | Article |
| id | mit-1721.1/88985 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:13:31Z |
| publishDate | 2014 |
| publisher | American Chemical Society |
| record_format | dspace |
| spelling | mit-1721.1/889852022-10-02T01:28:52Z Quantifying Particle Coatings Using High-Precision Mass Measurements Knudsen, Scott Michael von Muhlen, Marcio G. Manalis, Scott R. Massachusetts Institute of Technology. Department of Biological Engineering Knudsen, Scott Michael von Muhlen, Marcio G. Manalis, Scott R. We present a general method to quantify coatings on microparticle surfaces based on the additional mass. Particle buoyant mass is determined in a solution with a density that is nearly equivalent to that of the core particle, reducing the magnitude and uncertainty of the measurement. Under these conditions, added material with a different density than that of the core is a larger fraction of the total buoyant mass of the coated particle. This method can resolve a buoyant mass difference between uncoated and coated particles of 1 fg. For the protein layer on the 3 μm polystyrene spheres measured herein, this is equivalent to 1/10th of a full layer. United States. Army Research Office (Institute for Collaborative Biotechnologies, Contract No. W911NF- 09-D-0001) National Cancer Institute (U.S.) (Contract No. R01CA119402) 2014-08-22T16:12:23Z 2014-08-22T16:12:23Z 2012-02 2012-01 Article http://purl.org/eprint/type/JournalArticle 0003-2700 1520-6882 http://hdl.handle.net/1721.1/88985 Knudsen, Scott M., Marcio G. von Muhlen, and Scott R. Manalis. “Quantifying Particle Coatings Using High-Precision Mass Measurements.” Analytical Chemistry 84, no. 3 (February 7, 2012): 1240–1242. https://orcid.org/0000-0001-5223-9433 en_US http://dx.doi.org/10.1021/ac300034r Analytical Chemistry Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Chemical Society PMC |
| spellingShingle | Knudsen, Scott Michael von Muhlen, Marcio G. Manalis, Scott R. Quantifying Particle Coatings Using High-Precision Mass Measurements |
| title | Quantifying Particle Coatings Using High-Precision Mass Measurements |
| title_full | Quantifying Particle Coatings Using High-Precision Mass Measurements |
| title_fullStr | Quantifying Particle Coatings Using High-Precision Mass Measurements |
| title_full_unstemmed | Quantifying Particle Coatings Using High-Precision Mass Measurements |
| title_short | Quantifying Particle Coatings Using High-Precision Mass Measurements |
| title_sort | quantifying particle coatings using high precision mass measurements |
| url | http://hdl.handle.net/1721.1/88985 https://orcid.org/0000-0001-5223-9433 |
| work_keys_str_mv | AT knudsenscottmichael quantifyingparticlecoatingsusinghighprecisionmassmeasurements AT vonmuhlenmarciog quantifyingparticlecoatingsusinghighprecisionmassmeasurements AT manalisscottr quantifyingparticlecoatingsusinghighprecisionmassmeasurements |