Harnessing glycomics technologies: Integrating structure with function for glycan characterization
Glycans, or complex carbohydrates, are a ubiquitous class of biological molecule which impinge on a variety of physiological processes ranging from signal transduction to tissue development and microbial pathogenesis. In comparison to DNA and proteins, glycans present unique challenges to the study...
| Main Authors: | , , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Wiley Blackwell
2014
|
| Online Access: | http://hdl.handle.net/1721.1/89055 https://orcid.org/0000-0001-9344-0205 https://orcid.org/0000-0002-2085-7840 |
| _version_ | 1826198416571498496 |
|---|---|
| author | Artpradit, Charlermchai Raman, Rahul Shriver, Zachary H. Ruchirawat, Mathuros Sasisekharan, Ram Robinson, Luke Nathaniel |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Artpradit, Charlermchai Raman, Rahul Shriver, Zachary H. Ruchirawat, Mathuros Sasisekharan, Ram Robinson, Luke Nathaniel |
| author_sort | Artpradit, Charlermchai |
| collection | MIT |
| description | Glycans, or complex carbohydrates, are a ubiquitous class of biological molecule which impinge on a variety of physiological processes ranging from signal transduction to tissue development and microbial pathogenesis. In comparison to DNA and proteins, glycans present unique challenges to the study of their structure and function owing to their complex and heterogeneous structures and the dominant role played by multivalency in their sequence-specific biological interactions. Arising from these challenges, there is a need to integrate information from multiple complementary methods to decode structure–function relationships. Focusing on acidic glycans, we describe here key glycomics technologies for characterizing their structural attributes, including linkage, modifications, and topology, as well as for elucidating their role in biological processes. Two cases studies, one involving sialylated branched glycans and the other sulfated glycosaminoglycans, are used to highlight how integration of orthogonal information from diverse datasets enables rapid convergence of glycan characterization for development of robust structure–function relationships. |
| first_indexed | 2024-09-23T11:04:33Z |
| format | Article |
| id | mit-1721.1/89055 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:04:33Z |
| publishDate | 2014 |
| publisher | Wiley Blackwell |
| record_format | dspace |
| spelling | mit-1721.1/890552022-10-01T00:58:50Z Harnessing glycomics technologies: Integrating structure with function for glycan characterization Artpradit, Charlermchai Raman, Rahul Shriver, Zachary H. Ruchirawat, Mathuros Sasisekharan, Ram Robinson, Luke Nathaniel Harvard University--MIT Division of Health Sciences and Technology Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. School of Engineering Koch Institute for Integrative Cancer Research at MIT Robinson, Luke N. Raman, Rahul Shriver, Zachary H. Sasisekharan, Ram Glycans, or complex carbohydrates, are a ubiquitous class of biological molecule which impinge on a variety of physiological processes ranging from signal transduction to tissue development and microbial pathogenesis. In comparison to DNA and proteins, glycans present unique challenges to the study of their structure and function owing to their complex and heterogeneous structures and the dominant role played by multivalency in their sequence-specific biological interactions. Arising from these challenges, there is a need to integrate information from multiple complementary methods to decode structure–function relationships. Focusing on acidic glycans, we describe here key glycomics technologies for characterizing their structural attributes, including linkage, modifications, and topology, as well as for elucidating their role in biological processes. Two cases studies, one involving sialylated branched glycans and the other sulfated glycosaminoglycans, are used to highlight how integration of orthogonal information from diverse datasets enables rapid convergence of glycan characterization for development of robust structure–function relationships. National Institutes of Health (U.S.) (GM R37 GM057073-13) Singapore-MIT Alliance for Research and Technology 2014-08-26T14:35:31Z 2014-08-26T14:35:31Z 2012-04 2011-11 Article http://purl.org/eprint/type/JournalArticle 01730835 1522-2683 http://hdl.handle.net/1721.1/89055 Robinson, Luke N., Charlermchai Artpradit, Rahul Raman, Zachary H. Shriver, Mathuros Ruchirawat, and Ram Sasisekharan. “Harnessing Glycomics Technologies: Integrating Structure with Function for Glycan Characterization.” ELECTROPHORESIS 33, no. 5 (March 2012): 797–814. https://orcid.org/0000-0001-9344-0205 https://orcid.org/0000-0002-2085-7840 en_US http://dx.doi.org/10.1002/elps.201100231 ELECTROPHORESIS Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Wiley Blackwell PMC |
| spellingShingle | Artpradit, Charlermchai Raman, Rahul Shriver, Zachary H. Ruchirawat, Mathuros Sasisekharan, Ram Robinson, Luke Nathaniel Harnessing glycomics technologies: Integrating structure with function for glycan characterization |
| title | Harnessing glycomics technologies: Integrating structure with function for glycan characterization |
| title_full | Harnessing glycomics technologies: Integrating structure with function for glycan characterization |
| title_fullStr | Harnessing glycomics technologies: Integrating structure with function for glycan characterization |
| title_full_unstemmed | Harnessing glycomics technologies: Integrating structure with function for glycan characterization |
| title_short | Harnessing glycomics technologies: Integrating structure with function for glycan characterization |
| title_sort | harnessing glycomics technologies integrating structure with function for glycan characterization |
| url | http://hdl.handle.net/1721.1/89055 https://orcid.org/0000-0001-9344-0205 https://orcid.org/0000-0002-2085-7840 |
| work_keys_str_mv | AT artpraditcharlermchai harnessingglycomicstechnologiesintegratingstructurewithfunctionforglycancharacterization AT ramanrahul harnessingglycomicstechnologiesintegratingstructurewithfunctionforglycancharacterization AT shriverzacharyh harnessingglycomicstechnologiesintegratingstructurewithfunctionforglycancharacterization AT ruchirawatmathuros harnessingglycomicstechnologiesintegratingstructurewithfunctionforglycancharacterization AT sasisekharanram harnessingglycomicstechnologiesintegratingstructurewithfunctionforglycancharacterization AT robinsonlukenathaniel harnessingglycomicstechnologiesintegratingstructurewithfunctionforglycancharacterization |