Biased migration of confined neutrophil-like cells in asymmetric hydraulic environments
Cells integrate multiple measurement modalities to navigate their environment. Soluble and substrate-bound chemical gradients and physical cues have all been shown to influence cell orientation and migration. Here we investigate the role of asymmetric hydraulic pressure in directional sensing. Cells...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | en_US |
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National Academy of Sciences (U.S.)
2014
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| Online Access: | http://hdl.handle.net/1721.1/89089 |
| _version_ | 1826190109845749760 |
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| author | Mahadevan, L. Prentice-Mott, Harrison V. Chang, Chi-Han Mitchison, Timothy J. Irimia, Daniel Shah, Jagesh Vijaykumar |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Mahadevan, L. Prentice-Mott, Harrison V. Chang, Chi-Han Mitchison, Timothy J. Irimia, Daniel Shah, Jagesh Vijaykumar |
| author_sort | Mahadevan, L. |
| collection | MIT |
| description | Cells integrate multiple measurement modalities to navigate their environment. Soluble and substrate-bound chemical gradients and physical cues have all been shown to influence cell orientation and migration. Here we investigate the role of asymmetric hydraulic pressure in directional sensing. Cells confined in microchannels identified and chose a path of lower hydraulic resistance in the absence of chemical cues. In a bifurcating channel with asymmetric hydraulic resistances, this choice was preceded by the elaboration of two leading edges with a faster extension rate along the lower resistance channel. Retraction of the “losing” edge appeared to precipitate a final choice of direction. The pressure differences altering leading edge protrusion rates were small, suggesting weak force generation by leading edges. The response to the physical asymmetry was able to override a dynamically generated chemical cue. Motile cells may use this bias as a result of hydraulic resistance, or “barotaxis,” in concert with chemotaxis to navigate complex environments. |
| first_indexed | 2024-09-23T08:35:11Z |
| format | Article |
| id | mit-1721.1/89089 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T08:35:11Z |
| publishDate | 2014 |
| publisher | National Academy of Sciences (U.S.) |
| record_format | dspace |
| spelling | mit-1721.1/890892022-09-23T13:05:00Z Biased migration of confined neutrophil-like cells in asymmetric hydraulic environments Mahadevan, L. Prentice-Mott, Harrison V. Chang, Chi-Han Mitchison, Timothy J. Irimia, Daniel Shah, Jagesh Vijaykumar Harvard University--MIT Division of Health Sciences and Technology Prentice-Mott, Harrison V. Chang, Chi-Han Shah, Jagesh Vijaykumar Cells integrate multiple measurement modalities to navigate their environment. Soluble and substrate-bound chemical gradients and physical cues have all been shown to influence cell orientation and migration. Here we investigate the role of asymmetric hydraulic pressure in directional sensing. Cells confined in microchannels identified and chose a path of lower hydraulic resistance in the absence of chemical cues. In a bifurcating channel with asymmetric hydraulic resistances, this choice was preceded by the elaboration of two leading edges with a faster extension rate along the lower resistance channel. Retraction of the “losing” edge appeared to precipitate a final choice of direction. The pressure differences altering leading edge protrusion rates were small, suggesting weak force generation by leading edges. The response to the physical asymmetry was able to override a dynamically generated chemical cue. Motile cells may use this bias as a result of hydraulic resistance, or “barotaxis,” in concert with chemotaxis to navigate complex environments. 2014-08-28T16:34:24Z 2014-08-28T16:34:24Z 2013-12 2013-09 Article http://purl.org/eprint/type/JournalArticle 0027-8424 1091-6490 http://hdl.handle.net/1721.1/89089 Prentice-Mott, H. V., C.-H. Chang, L. Mahadevan, T. J. Mitchison, D. Irimia, and J. V. Shah. “Biased Migration of Confined Neutrophil-Like Cells in Asymmetric Hydraulic Environments.” Proceedings of the National Academy of Sciences 110, no. 52 (December 24, 2013): 21006–21011. en_US http://dx.doi.org/10.1073/pnas.1317441110 Proceedings of the National Academy of Sciences Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf National Academy of Sciences (U.S.) PNAS |
| spellingShingle | Mahadevan, L. Prentice-Mott, Harrison V. Chang, Chi-Han Mitchison, Timothy J. Irimia, Daniel Shah, Jagesh Vijaykumar Biased migration of confined neutrophil-like cells in asymmetric hydraulic environments |
| title | Biased migration of confined neutrophil-like cells in asymmetric hydraulic environments |
| title_full | Biased migration of confined neutrophil-like cells in asymmetric hydraulic environments |
| title_fullStr | Biased migration of confined neutrophil-like cells in asymmetric hydraulic environments |
| title_full_unstemmed | Biased migration of confined neutrophil-like cells in asymmetric hydraulic environments |
| title_short | Biased migration of confined neutrophil-like cells in asymmetric hydraulic environments |
| title_sort | biased migration of confined neutrophil like cells in asymmetric hydraulic environments |
| url | http://hdl.handle.net/1721.1/89089 |
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