PIP[subscript 3] Regulates Spinule Formation in Dendritic Spines during Structural Long-Term Potentiation
Dendritic spines are small, highly motile structures on dendritic shafts that provide flexibility to neuronal networks. Spinules are small protrusions that project from spines. The number and the length of spinules increase in response to activity including theta burst stimulation and glutamate appl...
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| Format: | Article |
| Language: | en_US |
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Society for Neuroscience
2014
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| Online Access: | http://hdl.handle.net/1721.1/89131 |
| _version_ | 1826193684004077568 |
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| author | Hayashi, Yasunori Ueda, Yoshibumi |
| author2 | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences |
| author_facet | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Hayashi, Yasunori Ueda, Yoshibumi |
| author_sort | Hayashi, Yasunori |
| collection | MIT |
| description | Dendritic spines are small, highly motile structures on dendritic shafts that provide flexibility to neuronal networks. Spinules are small protrusions that project from spines. The number and the length of spinules increase in response to activity including theta burst stimulation and glutamate application. However, what function spinules exert and how their formation is regulated still remains unclear. Phosphatidylinositol-3,4,5-trisphosphate (PIP[subscript 3]) plays important roles in cell motility such as filopodia and lamellipodia by recruiting downstream proteins such as Akt and WAVE to the membrane, respectively. Here we reveal that PIP[subscript 3] regulates spinule formation during structural long-term potentiation (sLTP) of single spines in CA1 pyramidal neurons of hippocampal slices from rats. Since the local distribution of PIP[subscript 3] is important to exert its functions, the subcellular distribution of PIP[subscript 3] was investigated using a fluorescence lifetime-based PIP[subscript 3] probe. PIP[subscript 3] accumulates to a greater extent in spines than in dendritic shafts, which is regulated by the subcellular activity pattern of proteins that produce and degrade PIP[subscript 3]. Subspine imaging revealed that when sLTP was induced in a single spine, PIP[subscript 3] accumulates in the spinule whereas PIP[subscript 3] concentration in the spine decreased. |
| first_indexed | 2024-09-23T09:43:03Z |
| format | Article |
| id | mit-1721.1/89131 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:43:03Z |
| publishDate | 2014 |
| publisher | Society for Neuroscience |
| record_format | dspace |
| spelling | mit-1721.1/891312022-09-30T16:22:48Z PIP[subscript 3] Regulates Spinule Formation in Dendritic Spines during Structural Long-Term Potentiation Hayashi, Yasunori Ueda, Yoshibumi Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Picower Institute for Learning and Memory RIKEN-MIT Neuroscience Research Center Hayashi, Yasunori Ueda, Yoshibumi Dendritic spines are small, highly motile structures on dendritic shafts that provide flexibility to neuronal networks. Spinules are small protrusions that project from spines. The number and the length of spinules increase in response to activity including theta burst stimulation and glutamate application. However, what function spinules exert and how their formation is regulated still remains unclear. Phosphatidylinositol-3,4,5-trisphosphate (PIP[subscript 3]) plays important roles in cell motility such as filopodia and lamellipodia by recruiting downstream proteins such as Akt and WAVE to the membrane, respectively. Here we reveal that PIP[subscript 3] regulates spinule formation during structural long-term potentiation (sLTP) of single spines in CA1 pyramidal neurons of hippocampal slices from rats. Since the local distribution of PIP[subscript 3] is important to exert its functions, the subcellular distribution of PIP[subscript 3] was investigated using a fluorescence lifetime-based PIP[subscript 3] probe. PIP[subscript 3] accumulates to a greater extent in spines than in dendritic shafts, which is regulated by the subcellular activity pattern of proteins that produce and degrade PIP[subscript 3]. Subspine imaging revealed that when sLTP was induced in a single spine, PIP[subscript 3] accumulates in the spinule whereas PIP[subscript 3] concentration in the spine decreased. National Institutes of Health (U.S.) (Grant R01DA17310) 2014-09-02T16:53:59Z 2014-09-02T16:53:59Z 2013-07 2013-05 Article http://purl.org/eprint/type/JournalArticle 0270-6474 1529-2401 http://hdl.handle.net/1721.1/89131 Ueda, Y., and Y. Hayashi. “PIP[subscript 3] Regulates Spinule Formation in Dendritic Spines During Structural Long-Term Potentiation.” Journal of Neuroscience 33, no. 27 (July 3, 2013): 11040–11047. en_US http://dx.doi.org/10.1523/jneurosci.3122-12.2013 Journal of Neuroscience Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Society for Neuroscience Society for Neuroscience |
| spellingShingle | Hayashi, Yasunori Ueda, Yoshibumi PIP[subscript 3] Regulates Spinule Formation in Dendritic Spines during Structural Long-Term Potentiation |
| title | PIP[subscript 3] Regulates Spinule Formation in Dendritic Spines during Structural Long-Term Potentiation |
| title_full | PIP[subscript 3] Regulates Spinule Formation in Dendritic Spines during Structural Long-Term Potentiation |
| title_fullStr | PIP[subscript 3] Regulates Spinule Formation in Dendritic Spines during Structural Long-Term Potentiation |
| title_full_unstemmed | PIP[subscript 3] Regulates Spinule Formation in Dendritic Spines during Structural Long-Term Potentiation |
| title_short | PIP[subscript 3] Regulates Spinule Formation in Dendritic Spines during Structural Long-Term Potentiation |
| title_sort | pip subscript 3 regulates spinule formation in dendritic spines during structural long term potentiation |
| url | http://hdl.handle.net/1721.1/89131 |
| work_keys_str_mv | AT hayashiyasunori pipsubscript3regulatesspinuleformationindendriticspinesduringstructurallongtermpotentiation AT uedayoshibumi pipsubscript3regulatesspinuleformationindendriticspinesduringstructurallongtermpotentiation |