Meiosis I chromosome segregation is established through regulation of microtubule–kinetochore interactions
During meiosis, a single round of DNA replication is followed by two consecutive rounds of nuclear divisions called meiosis I and meiosis II. In meiosis I, homologous chromosomes segregate, while sister chromatids remain together. Determining how this unusual chromosome segregation behavior is estab...
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| Format: | Article |
| Language: | en_US |
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eLife Sciences Publications, Ltd.
2014
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| Online Access: | http://hdl.handle.net/1721.1/89177 https://orcid.org/0000-0003-2012-7546 https://orcid.org/0000-0001-9837-0314 |
| _version_ | 1826215906897821696 |
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| author | Miller, Matthew P. Unal, Elcin Brar, Gloria A. Amon, Angelika B |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Miller, Matthew P. Unal, Elcin Brar, Gloria A. Amon, Angelika B |
| author_sort | Miller, Matthew P. |
| collection | MIT |
| description | During meiosis, a single round of DNA replication is followed by two consecutive rounds of nuclear divisions called meiosis I and meiosis II. In meiosis I, homologous chromosomes segregate, while sister chromatids remain together. Determining how this unusual chromosome segregation behavior is established is central to understanding germ cell development. Here we show that preventing microtubule–kinetochore interactions during premeiotic S phase and prophase I is essential for establishing the meiosis I chromosome segregation pattern. Premature interactions of kinetochores with microtubules transform meiosis I into a mitosis-like division by disrupting two key meiosis I events: coorientation of sister kinetochores and protection of centromeric cohesin removal from chromosomes. Furthermore we find that restricting outer kinetochore assembly contributes to preventing premature engagement of microtubules with kinetochores. We propose that inhibition of microtubule–kinetochore interactions during premeiotic S phase and prophase I is central to establishing the unique meiosis I chromosome segregation pattern. |
| first_indexed | 2024-09-23T16:38:55Z |
| format | Article |
| id | mit-1721.1/89177 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T16:38:55Z |
| publishDate | 2014 |
| publisher | eLife Sciences Publications, Ltd. |
| record_format | dspace |
| spelling | mit-1721.1/891772022-10-03T07:22:16Z Meiosis I chromosome segregation is established through regulation of microtubule–kinetochore interactions Miller, Matthew P. Unal, Elcin Brar, Gloria A. Amon, Angelika B Massachusetts Institute of Technology. Department of Biology Miller, Matthew P. Unal, Elcin Amon, Angelika B. During meiosis, a single round of DNA replication is followed by two consecutive rounds of nuclear divisions called meiosis I and meiosis II. In meiosis I, homologous chromosomes segregate, while sister chromatids remain together. Determining how this unusual chromosome segregation behavior is established is central to understanding germ cell development. Here we show that preventing microtubule–kinetochore interactions during premeiotic S phase and prophase I is essential for establishing the meiosis I chromosome segregation pattern. Premature interactions of kinetochores with microtubules transform meiosis I into a mitosis-like division by disrupting two key meiosis I events: coorientation of sister kinetochores and protection of centromeric cohesin removal from chromosomes. Furthermore we find that restricting outer kinetochore assembly contributes to preventing premature engagement of microtubules with kinetochores. We propose that inhibition of microtubule–kinetochore interactions during premeiotic S phase and prophase I is central to establishing the unique meiosis I chromosome segregation pattern. Howard Hughes Medical Institute National Institutes of Health (U.S.) (grant GM62207) Jane Coffin Childs Memorial Fund for Medical Research American Cancer Society 2014-09-04T18:13:42Z 2014-09-04T18:13:42Z 2012-12 2012-07 Article http://purl.org/eprint/type/JournalArticle 2050-084X http://hdl.handle.net/1721.1/89177 Miller, Matthew P, Elçin Ünal, Gloria A Brar, and Angelika Amon. “Meiosis I Chromosome Segregation Is Established through Regulation of Microtubule–kinetochore Interactions.” eLife;1:e00117 (December 18, 2012). https://orcid.org/0000-0003-2012-7546 https://orcid.org/0000-0001-9837-0314 en_US http://dx.doi.org/10.7554/eLife.00117 eLife Creative Commons Attribution http://creativecommons.org/licenses/by/3.0/ application/pdf eLife Sciences Publications, Ltd. eLife Sciences Publications, Ltd. |
| spellingShingle | Miller, Matthew P. Unal, Elcin Brar, Gloria A. Amon, Angelika B Meiosis I chromosome segregation is established through regulation of microtubule–kinetochore interactions |
| title | Meiosis I chromosome segregation is established through regulation of microtubule–kinetochore interactions |
| title_full | Meiosis I chromosome segregation is established through regulation of microtubule–kinetochore interactions |
| title_fullStr | Meiosis I chromosome segregation is established through regulation of microtubule–kinetochore interactions |
| title_full_unstemmed | Meiosis I chromosome segregation is established through regulation of microtubule–kinetochore interactions |
| title_short | Meiosis I chromosome segregation is established through regulation of microtubule–kinetochore interactions |
| title_sort | meiosis i chromosome segregation is established through regulation of microtubule kinetochore interactions |
| url | http://hdl.handle.net/1721.1/89177 https://orcid.org/0000-0003-2012-7546 https://orcid.org/0000-0001-9837-0314 |
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