Feasibility of the Assessment of Cholesterol Crystals in Human Macrophages Using Micro Optical Coherence Tomography
The presence of cholesterol crystals is a hallmark of atherosclerosis, but until recently, such crystals have been considered to be passive components of necrotic plaque cores. Recent studies have demonstrated that phagocytosis of cholesterol crystals by macrophages may actively precipitate plaque p...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Public Library of Science
2014
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| Online Access: | http://hdl.handle.net/1721.1/89449 |
| _version_ | 1811081848795693056 |
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| author | Kashiwagi, Manabu Liu, Linbo Chu, Kengyeh K. Sun, Chen-Hsin Tanaka, Atsushi Gardecki, Joseph A. Tearney, Guillermo J. |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Kashiwagi, Manabu Liu, Linbo Chu, Kengyeh K. Sun, Chen-Hsin Tanaka, Atsushi Gardecki, Joseph A. Tearney, Guillermo J. |
| author_sort | Kashiwagi, Manabu |
| collection | MIT |
| description | The presence of cholesterol crystals is a hallmark of atherosclerosis, but until recently, such crystals have been considered to be passive components of necrotic plaque cores. Recent studies have demonstrated that phagocytosis of cholesterol crystals by macrophages may actively precipitate plaque progression via an inflammatory pathway, emphasizing the need for methods to study the interaction between macrophages and crystalline cholesterol. In this study, we demonstrate the feasibility of detecting cholesterol in macrophages in situ using Micro-Optical Coherence Tomography (µOCT), an imaging modality we have recently developed with 1-µm resolution. Macrophages containing cholesterol crystals frequently demonstrated highly scattering constituents in their cytoplasm on µOCT imaging, and µOCT was able to evaluate cholesterol crystals in cultured macrophage cells. Our results suggest that µOCT may be useful for the detection and characterization of inflammatory activity associated with cholesterol crystals in the coronary artery. |
| first_indexed | 2024-09-23T11:53:26Z |
| format | Article |
| id | mit-1721.1/89449 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:53:26Z |
| publishDate | 2014 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/894492022-10-01T06:43:55Z Feasibility of the Assessment of Cholesterol Crystals in Human Macrophages Using Micro Optical Coherence Tomography Kashiwagi, Manabu Liu, Linbo Chu, Kengyeh K. Sun, Chen-Hsin Tanaka, Atsushi Gardecki, Joseph A. Tearney, Guillermo J. Harvard University--MIT Division of Health Sciences and Technology Tearney, Guillermo J. The presence of cholesterol crystals is a hallmark of atherosclerosis, but until recently, such crystals have been considered to be passive components of necrotic plaque cores. Recent studies have demonstrated that phagocytosis of cholesterol crystals by macrophages may actively precipitate plaque progression via an inflammatory pathway, emphasizing the need for methods to study the interaction between macrophages and crystalline cholesterol. In this study, we demonstrate the feasibility of detecting cholesterol in macrophages in situ using Micro-Optical Coherence Tomography (µOCT), an imaging modality we have recently developed with 1-µm resolution. Macrophages containing cholesterol crystals frequently demonstrated highly scattering constituents in their cytoplasm on µOCT imaging, and µOCT was able to evaluate cholesterol crystals in cultured macrophage cells. Our results suggest that µOCT may be useful for the detection and characterization of inflammatory activity associated with cholesterol crystals in the coronary artery. National Heart, Lung, and Blood Institute (Award R01HL076398) National Heart, Lung, and Blood Institute (Award R01HL093717) 2014-09-11T18:50:50Z 2014-09-11T18:50:50Z 2014-07 2014-01 Article http://purl.org/eprint/type/JournalArticle 1932-6203 http://hdl.handle.net/1721.1/89449 Kashiwagi, Manabu, Linbo Liu, Kengyeh K. Chu, Chen-Hsin Sun, Atsushi Tanaka, Joseph A. Gardecki, and Guillermo J. Tearney. “Feasibility of the Assessment of Cholesterol Crystals in Human Macrophages Using Micro Optical Coherence Tomography.” Edited by Marc van Zandvoort. PLoS ONE 9, no. 7 (July 21, 2014): e102669. en_US http://dx.doi.org/10.1371/journal.pone.0102669 PLoS ONE Creative Commons Attribution http://creativecommons.org/licenses/by/4.0/ application/pdf Public Library of Science Public Library of Science |
| spellingShingle | Kashiwagi, Manabu Liu, Linbo Chu, Kengyeh K. Sun, Chen-Hsin Tanaka, Atsushi Gardecki, Joseph A. Tearney, Guillermo J. Feasibility of the Assessment of Cholesterol Crystals in Human Macrophages Using Micro Optical Coherence Tomography |
| title | Feasibility of the Assessment of Cholesterol Crystals in Human Macrophages Using Micro Optical Coherence Tomography |
| title_full | Feasibility of the Assessment of Cholesterol Crystals in Human Macrophages Using Micro Optical Coherence Tomography |
| title_fullStr | Feasibility of the Assessment of Cholesterol Crystals in Human Macrophages Using Micro Optical Coherence Tomography |
| title_full_unstemmed | Feasibility of the Assessment of Cholesterol Crystals in Human Macrophages Using Micro Optical Coherence Tomography |
| title_short | Feasibility of the Assessment of Cholesterol Crystals in Human Macrophages Using Micro Optical Coherence Tomography |
| title_sort | feasibility of the assessment of cholesterol crystals in human macrophages using micro optical coherence tomography |
| url | http://hdl.handle.net/1721.1/89449 |
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