Cell Patterning with Mucin Biopolymers
The precise spatial control of cell adhesion to surfaces is an endeavor that has enabled discoveries in cell biology and new possibilities in tissue engineering. The generation of cell-repellent surfaces currently requires advanced chemistry techniques and could be simplified. Here we show that muci...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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American Chemical Society (ACS)
2014
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| Online Access: | http://hdl.handle.net/1721.1/89634 https://orcid.org/0000-0001-8260-338X https://orcid.org/0000-0002-3199-0508 |
| _version_ | 1811091169040400384 |
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| author | Crouzier, T. Jang, H. Ahn, J. Stocker, Roman Ribbeck, Katharina |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Crouzier, T. Jang, H. Ahn, J. Stocker, Roman Ribbeck, Katharina |
| author_sort | Crouzier, T. |
| collection | MIT |
| description | The precise spatial control of cell adhesion to surfaces is an endeavor that has enabled discoveries in cell biology and new possibilities in tissue engineering. The generation of cell-repellent surfaces currently requires advanced chemistry techniques and could be simplified. Here we show that mucins, glycoproteins of high structural and chemical complexity, spontaneously adsorb on hydrophobic substrates to form coatings that prevent the surface adhesion of mammalian epithelial cells, fibroblasts, and myoblasts. These mucin coatings can be patterned with micrometer precision using a microfluidic device, and are stable enough to support myoblast differentiation over seven days. Moreover, our data indicate that the cell-repellent effect is dependent on mucin-associated glycans because their removal results in a loss of effective cell-repulsion. Last, we show that a critical surface density of mucins, which is required to achieve cell-repulsion, is efficiently obtained on hydrophobic surfaces, but not on hydrophilic glass surfaces. However, this limitation can be overcome by coating glass with hydrophobic fluorosilane. We conclude that mucin biopolymers are attractive candidates to control cell adhesion on surfaces. |
| first_indexed | 2024-09-23T14:57:43Z |
| format | Article |
| id | mit-1721.1/89634 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:57:43Z |
| publishDate | 2014 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/896342022-10-01T23:37:09Z Cell Patterning with Mucin Biopolymers Crouzier, T. Jang, H. Ahn, J. Stocker, Roman Ribbeck, Katharina Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Civil and Environmental Engineering Stocker, Roman Ribbeck, Katharina Crouzier, T. Jang, H. Ahn, J. The precise spatial control of cell adhesion to surfaces is an endeavor that has enabled discoveries in cell biology and new possibilities in tissue engineering. The generation of cell-repellent surfaces currently requires advanced chemistry techniques and could be simplified. Here we show that mucins, glycoproteins of high structural and chemical complexity, spontaneously adsorb on hydrophobic substrates to form coatings that prevent the surface adhesion of mammalian epithelial cells, fibroblasts, and myoblasts. These mucin coatings can be patterned with micrometer precision using a microfluidic device, and are stable enough to support myoblast differentiation over seven days. Moreover, our data indicate that the cell-repellent effect is dependent on mucin-associated glycans because their removal results in a loss of effective cell-repulsion. Last, we show that a critical surface density of mucins, which is required to achieve cell-repulsion, is efficiently obtained on hydrophobic surfaces, but not on hydrophilic glass surfaces. However, this limitation can be overcome by coating glass with hydrophobic fluorosilane. We conclude that mucin biopolymers are attractive candidates to control cell adhesion on surfaces. European Commission (Marie Curie International Outgoing Fellowship for Career Development, “BIOMUC”) National Institutes of Health (U.S.) (NIH Grant 1R01GM100473) National Science Foundation (U.S.) (award number DMR-819762) National Science Foundation (U.S.) (NSF Grant OCE-0744641-CAREER) National Science Foundation (U.S.) (Award DMR-0819762) Samsung Scholarship Foundation Massachusetts Institute of Technology (Startup funds) Massachusetts Institute of Technology (Junior Faculty award) 2014-09-15T19:21:32Z 2014-09-15T19:21:32Z 2013-09 2013-06 Article http://purl.org/eprint/type/JournalArticle 1525-7797 1526-4602 http://hdl.handle.net/1721.1/89634 Crouzier, T., H. Jang, J. Ahn, R. Stocker, and K. Ribbeck. “Cell Patterning with Mucin Biopolymers.” Biomacromolecules 14, no. 9 (September 9, 2013): 3010–3016. https://orcid.org/0000-0001-8260-338X https://orcid.org/0000-0002-3199-0508 en_US http://dx.doi.org/10.1021/bm400447z Biomacromolecules Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf American Chemical Society (ACS) PMC |
| spellingShingle | Crouzier, T. Jang, H. Ahn, J. Stocker, Roman Ribbeck, Katharina Cell Patterning with Mucin Biopolymers |
| title | Cell Patterning with Mucin Biopolymers |
| title_full | Cell Patterning with Mucin Biopolymers |
| title_fullStr | Cell Patterning with Mucin Biopolymers |
| title_full_unstemmed | Cell Patterning with Mucin Biopolymers |
| title_short | Cell Patterning with Mucin Biopolymers |
| title_sort | cell patterning with mucin biopolymers |
| url | http://hdl.handle.net/1721.1/89634 https://orcid.org/0000-0001-8260-338X https://orcid.org/0000-0002-3199-0508 |
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