Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform
Rapid mechanical deformation of cells has emerged as a promising, vector-free method for intracellular delivery of macromolecules and nanomaterials. This technology has shown potential in addressing previously challenging applications; including, delivery to primary immune cells, cell reprogramming,...
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| Format: | Article |
| Language: | en_US |
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MyJoVE Corporation
2014
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| Online Access: | http://hdl.handle.net/1721.1/89648 https://orcid.org/0000-0001-7192-580X https://orcid.org/0000-0003-4255-0492 |
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| author | Sharei, Armon Reza Cho, Nahyun Mao, Shirley Jackson, Emily L. Poceviciute, Roberta Zoldan, Janet Langer, Robert Jensen, Klavs F. Adamo, Andrea, 1975- |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Sharei, Armon Reza Cho, Nahyun Mao, Shirley Jackson, Emily L. Poceviciute, Roberta Zoldan, Janet Langer, Robert Jensen, Klavs F. Adamo, Andrea, 1975- |
| author_sort | Sharei, Armon Reza |
| collection | MIT |
| description | Rapid mechanical deformation of cells has emerged as a promising, vector-free method for intracellular delivery of macromolecules and nanomaterials. This technology has shown potential in addressing previously challenging applications; including, delivery to primary immune cells, cell reprogramming, carbon nanotube, and quantum dot delivery. This vector-free microfluidic platform relies on mechanical disruption of the cell membrane to facilitate cytosolic delivery of the target material. Herein, we describe the detailed method of use for these microfluidic devices including, device assembly, cell preparation, and system operation. This delivery approach requires a brief optimization of device type and operating conditions for previously unreported applications. The provided instructions are generalizable to most cell types and delivery materials as this system does not require specialized buffers or chemical modification/conjugation steps. This work also provides recommendations on how to improve device performance and trouble-shoot potential issues related to clogging, low delivery efficiencies, and cell viability. |
| first_indexed | 2024-09-23T12:42:26Z |
| format | Article |
| id | mit-1721.1/89648 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T12:42:26Z |
| publishDate | 2014 |
| publisher | MyJoVE Corporation |
| record_format | dspace |
| spelling | mit-1721.1/896482022-09-28T09:32:50Z Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform Sharei, Armon Reza Cho, Nahyun Mao, Shirley Jackson, Emily L. Poceviciute, Roberta Zoldan, Janet Langer, Robert Jensen, Klavs F. Adamo, Andrea, 1975- Massachusetts Institute of Technology. Department of Chemical Engineering Koch Institute for Integrative Cancer Research at MIT Sharei, Armon Reza Cho, Nahyun Mao, Shirley Jackson, Emily L. Poceviciute, Roberta Adamo, Andrea Zoldan, Janet Langer, Robert Jensen, Klavs F. Rapid mechanical deformation of cells has emerged as a promising, vector-free method for intracellular delivery of macromolecules and nanomaterials. This technology has shown potential in addressing previously challenging applications; including, delivery to primary immune cells, cell reprogramming, carbon nanotube, and quantum dot delivery. This vector-free microfluidic platform relies on mechanical disruption of the cell membrane to facilitate cytosolic delivery of the target material. Herein, we describe the detailed method of use for these microfluidic devices including, device assembly, cell preparation, and system operation. This delivery approach requires a brief optimization of device type and operating conditions for previously unreported applications. The provided instructions are generalizable to most cell types and delivery materials as this system does not require specialized buffers or chemical modification/conjugation steps. This work also provides recommendations on how to improve device performance and trouble-shoot potential issues related to clogging, low delivery efficiencies, and cell viability. National Institutes of Health (U.S.) (grant RC1 EB011187-02) National Institutes of Health (U.S.) (grant DE013023) National Institutes of Health (U.S.) (grant DE016516) National Institutes of Health (U.S.) (grant EB000351) National Cancer Institute (U.S.) (P30-CA14051) National Cancer Institute (U.S.) (Core grant MPP-09Call-Langer-60) 2014-09-16T16:52:10Z 2014-09-16T16:52:10Z 2013-11 Article http://purl.org/eprint/type/JournalArticle 1940-087X http://hdl.handle.net/1721.1/89648 Sharei, Armon, Nahyun Cho, Shirley Mao, Emily Jackson, Roberta Poceviciute, Andrea Adamo, Janet Zoldan, Robert Langer, and Klavs F Jensen. “Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform.” JoVE no. 81 (November 7, 2013). https://orcid.org/0000-0001-7192-580X https://orcid.org/0000-0003-4255-0492 en_US http://dx.doi.org/10.3791/50980 Journal of Visualized Experiments Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf MyJoVE Corporation MyJoVE Corporation |
| spellingShingle | Sharei, Armon Reza Cho, Nahyun Mao, Shirley Jackson, Emily L. Poceviciute, Roberta Zoldan, Janet Langer, Robert Jensen, Klavs F. Adamo, Andrea, 1975- Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform |
| title | Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform |
| title_full | Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform |
| title_fullStr | Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform |
| title_full_unstemmed | Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform |
| title_short | Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform |
| title_sort | cell squeezing as a robust microfluidic intracellular delivery platform |
| url | http://hdl.handle.net/1721.1/89648 https://orcid.org/0000-0001-7192-580X https://orcid.org/0000-0003-4255-0492 |
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