Mechanisms underlying rapid activity-dependent structural plasticity at Drosophila neuromuscular junctions
Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biology, 2014.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2014
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| Online Access: | http://hdl.handle.net/1721.1/89946 |
| _version_ | 1826211209617080320 |
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| author | Piccioli, Zachary D. (Zachary David) |
| author2 | J. Troy Littleton. |
| author_facet | J. Troy Littleton. Piccioli, Zachary D. (Zachary David) |
| author_sort | Piccioli, Zachary D. (Zachary David) |
| collection | MIT |
| description | Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biology, 2014. |
| first_indexed | 2024-09-23T15:02:18Z |
| format | Thesis |
| id | mit-1721.1/89946 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T15:02:18Z |
| publishDate | 2014 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/899462019-04-11T11:35:45Z Mechanisms underlying rapid activity-dependent structural plasticity at Drosophila neuromuscular junctions Piccioli, Zachary D. (Zachary David) J. Troy Littleton. Massachusetts Institute of Technology. Department of Biology. Massachusetts Institute of Technology. Department of Biology. Biology. Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biology, 2014. Cataloged from PDF version of thesis. Includes bibliographical references. The Drosophila neuromuscular junction (NMJ) is capable of rapidly budding new presynaptic varicosities over the course of minutes in response to elevated neuronal activity. Using live imaging of synaptic growth, we characterized this dynamic process and demonstrate that rapid bouton budding requires retrograde BMP signaling and local alteration in the presynaptic actin cytoskeleton. BMP acts during development to provide competence for rapid synaptic growth by regulating the levels of the Rho GEF trio, a transcriptional output of BMP-Smad signaling. In a parallel pathway, we find that the BMP type 11 receptor Wit signals through the effector protein LIM domain kinasel (Limk) to regulate bouton budding. Limk interfaces with structural plasticity by controlling the activity of the actin depolymerizing protein Cofilin. Expression of constitutively active or inactive Cofilin in motor neurons demonstrates that increased Cofilin activity promotes rapid bouton formation in response to elevated synaptic activity. Correspondingly, overexpression of Limk, which inhibits Cofilin, inhibits bouton budding. Live imaging of the presynaptic F-actin cytoskeleton reveals that activity-dependent bouton addition is accompanied by formation of new F-actin puncta at sites of synaptic growth. Pharmacological disruption of actin turnover inhibits bouton budding, indicating local changes in the actin cytoskeleton at preexisting boutons precede new budding events. We propose that developmental BMP signaling potentiates NMJs for rapid activity-dependent structural plasticity that is achieved by muscle release of retrograde signals that regulate local presynaptic actin cytoskeletal dynamics. by Zachary D. Piccioli. Ph. D. 2014-09-19T21:30:20Z 2014-09-19T21:30:20Z 2014 2014 Thesis http://hdl.handle.net/1721.1/89946 890124995 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 124 pages application/pdf Massachusetts Institute of Technology |
| spellingShingle | Biology. Piccioli, Zachary D. (Zachary David) Mechanisms underlying rapid activity-dependent structural plasticity at Drosophila neuromuscular junctions |
| title | Mechanisms underlying rapid activity-dependent structural plasticity at Drosophila neuromuscular junctions |
| title_full | Mechanisms underlying rapid activity-dependent structural plasticity at Drosophila neuromuscular junctions |
| title_fullStr | Mechanisms underlying rapid activity-dependent structural plasticity at Drosophila neuromuscular junctions |
| title_full_unstemmed | Mechanisms underlying rapid activity-dependent structural plasticity at Drosophila neuromuscular junctions |
| title_short | Mechanisms underlying rapid activity-dependent structural plasticity at Drosophila neuromuscular junctions |
| title_sort | mechanisms underlying rapid activity dependent structural plasticity at drosophila neuromuscular junctions |
| topic | Biology. |
| url | http://hdl.handle.net/1721.1/89946 |
| work_keys_str_mv | AT picciolizacharydzacharydavid mechanismsunderlyingrapidactivitydependentstructuralplasticityatdrosophilaneuromuscularjunctions |