Multiple Structural Maintenance of Chromosome Complexes at Transcriptional Regulatory Elements
Transcription factors control cell-specific gene expression programs by binding regulatory elements and recruiting cofactors and the transcription apparatus to the initiation sites of active genes. One of these cofactors is cohesin, a structural maintenance of chromosomes (SMC) complex that is neces...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Elsevier B.V.
2014
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| Online Access: | http://hdl.handle.net/1721.1/90570 https://orcid.org/0000-0001-8855-8647 |
| _version_ | 1811070743536992256 |
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| author | Dowen, Jill M. Bilodeau, Steve Orlando, David A. Hübner, Michael R. Abraham, Brian J. Spector, David L. Young, Richard A. Young, Richard A. |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Dowen, Jill M. Bilodeau, Steve Orlando, David A. Hübner, Michael R. Abraham, Brian J. Spector, David L. Young, Richard A. Young, Richard A. |
| author_sort | Dowen, Jill M. |
| collection | MIT |
| description | Transcription factors control cell-specific gene expression programs by binding regulatory elements and recruiting cofactors and the transcription apparatus to the initiation sites of active genes. One of these cofactors is cohesin, a structural maintenance of chromosomes (SMC) complex that is necessary for proper gene expression. We report that a second SMC complex, condensin II, is also present at transcriptional regulatory elements of active genes during interphase and is necessary for normal gene activity. Both cohesin and condensin II are associated with genes in euchromatin and not heterochromatin. The two SMC complexes and the SMC loading factor NIPBL are particularly enriched at super-enhancers, and the genes associated with these regulatory elements are especially sensitive to reduced levels of these complexes. Thus, in addition to their well-established functions in chromosome maintenance during mitosis, both cohesin and condensin II make important contributions to the functions of the key transcriptional regulatory elements during interphase. |
| first_indexed | 2024-09-23T08:40:52Z |
| format | Article |
| id | mit-1721.1/90570 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T08:40:52Z |
| publishDate | 2014 |
| publisher | Elsevier B.V. |
| record_format | dspace |
| spelling | mit-1721.1/905702022-09-30T10:26:43Z Multiple Structural Maintenance of Chromosome Complexes at Transcriptional Regulatory Elements Dowen, Jill M. Bilodeau, Steve Orlando, David A. Hübner, Michael R. Abraham, Brian J. Spector, David L. Young, Richard A. Young, Richard A. Massachusetts Institute of Technology. Department of Biology Young, Richard A. Transcription factors control cell-specific gene expression programs by binding regulatory elements and recruiting cofactors and the transcription apparatus to the initiation sites of active genes. One of these cofactors is cohesin, a structural maintenance of chromosomes (SMC) complex that is necessary for proper gene expression. We report that a second SMC complex, condensin II, is also present at transcriptional regulatory elements of active genes during interphase and is necessary for normal gene activity. Both cohesin and condensin II are associated with genes in euchromatin and not heterochromatin. The two SMC complexes and the SMC loading factor NIPBL are particularly enriched at super-enhancers, and the genes associated with these regulatory elements are especially sensitive to reduced levels of these complexes. Thus, in addition to their well-established functions in chromosome maintenance during mitosis, both cohesin and condensin II make important contributions to the functions of the key transcriptional regulatory elements during interphase. Canadian Institutes of Health Research (Fellowship) National Institutes of Health (U.S.) (grant GM42694) National Institutes of Health (U.S.) (grant HG002668) National Cancer Institute (U.S.) (NIH, Ruth L. Kirschstein National Research Service Award (CA168263-01A1)) 2014-10-07T15:00:07Z 2014-10-07T15:00:07Z 2013-11 Article http://purl.org/eprint/type/JournalArticle 22136711 http://hdl.handle.net/1721.1/90570 Dowen, Jill M., Steve Bilodeau, David A. Orlando, Michael R. Hübner, Brian J. Abraham, David L. Spector, and Richard A. Young. “Multiple Structural Maintenance of Chromosome Complexes at Transcriptional Regulatory Elements.” Stem Cell Reports 1, no. 5 (November 2013): 371–378. https://orcid.org/0000-0001-8855-8647 en_US http://dx.doi.org/10.1016/j.stemcr.2013.09.002 Stem Cell Reports Creative Commons Attribution http://creativecommons.org/licenses/by/3.0/ application/pdf Elsevier B.V. Elsevier |
| spellingShingle | Dowen, Jill M. Bilodeau, Steve Orlando, David A. Hübner, Michael R. Abraham, Brian J. Spector, David L. Young, Richard A. Young, Richard A. Multiple Structural Maintenance of Chromosome Complexes at Transcriptional Regulatory Elements |
| title | Multiple Structural Maintenance of Chromosome Complexes at Transcriptional Regulatory Elements |
| title_full | Multiple Structural Maintenance of Chromosome Complexes at Transcriptional Regulatory Elements |
| title_fullStr | Multiple Structural Maintenance of Chromosome Complexes at Transcriptional Regulatory Elements |
| title_full_unstemmed | Multiple Structural Maintenance of Chromosome Complexes at Transcriptional Regulatory Elements |
| title_short | Multiple Structural Maintenance of Chromosome Complexes at Transcriptional Regulatory Elements |
| title_sort | multiple structural maintenance of chromosome complexes at transcriptional regulatory elements |
| url | http://hdl.handle.net/1721.1/90570 https://orcid.org/0000-0001-8855-8647 |
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