Recognition of heparan sulfate by clinical strains of dengue virus serotype 1 using recombinant subviral particles
Dengue is the most important arthropod-borne viral disease in humans, with an estimated 3.6 billion people at risk for infection and more than 200 million infections per year. Identification of the cellular receptors for dengue virus (DV), the causative agent of dengue, is important toward understan...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | en_US |
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Elsevier
2014
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| Online Access: | http://hdl.handle.net/1721.1/90814 https://orcid.org/0000-0002-2085-7840 |
| _version_ | 1811074231208771584 |
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| author | Artpradit, Charlermchai Gavrilov, Boris K. Rurak, Troy T. Ruchirawat, Mathuros Sasisekharan, Ram Robinson, Luke Nathaniel |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Artpradit, Charlermchai Gavrilov, Boris K. Rurak, Troy T. Ruchirawat, Mathuros Sasisekharan, Ram Robinson, Luke Nathaniel |
| author_sort | Artpradit, Charlermchai |
| collection | MIT |
| description | Dengue is the most important arthropod-borne viral disease in humans, with an estimated 3.6 billion people at risk for infection and more than 200 million infections per year. Identification of the cellular receptors for dengue virus (DV), the causative agent of dengue, is important toward understanding the pathogenesis of the disease. Heparan sulfate (HS) has been characterized as a DV receptor in multiple model systems, however the physiological relevance of these findings has been questioned by observations that flaviviruses, including DV, can undergo cell culture adaptation changes resulting in increased binding to HS. It thus remains unclear whether HS is utilized by clinical, non-cell culture-adapted strains of DV. To address this question, herein we describe a set of methodologies using recombinant subviral particles (RSPs) to determine the utilization of HS by clinical strains of DV serotype 1 (DV1). RSPs of clinically isolated strains with low cell culture passage histories were used to study HS interaction. Biochemically characterized RSPs showed dose-dependent binding to immobilized heparin, which could be competed by heparin and HS but not structurally related glycosaminoglycans chondroitin sulfate A and hyaluronic acid. The relevance of heparin and HS biochemical interactions was demonstrated by competition of RSP and DV binding to cells with soluble heparin and HS. Our results demonstrate that clinical strains of DV1 can specifically interact with heparin and HS. Together, these data support the possibility that HS on cell surfaces is utilized in the DV-human infection process. |
| first_indexed | 2024-09-23T09:46:02Z |
| format | Article |
| id | mit-1721.1/90814 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:46:02Z |
| publishDate | 2014 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/908142022-09-26T13:33:28Z Recognition of heparan sulfate by clinical strains of dengue virus serotype 1 using recombinant subviral particles Artpradit, Charlermchai Gavrilov, Boris K. Rurak, Troy T. Ruchirawat, Mathuros Sasisekharan, Ram Robinson, Luke Nathaniel Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. School of Engineering Koch Institute for Integrative Cancer Research at MIT Robinson, Luke N. Gavrilov, Boris K. Rurak, Troy T. Sasisekharan, Ram Dengue is the most important arthropod-borne viral disease in humans, with an estimated 3.6 billion people at risk for infection and more than 200 million infections per year. Identification of the cellular receptors for dengue virus (DV), the causative agent of dengue, is important toward understanding the pathogenesis of the disease. Heparan sulfate (HS) has been characterized as a DV receptor in multiple model systems, however the physiological relevance of these findings has been questioned by observations that flaviviruses, including DV, can undergo cell culture adaptation changes resulting in increased binding to HS. It thus remains unclear whether HS is utilized by clinical, non-cell culture-adapted strains of DV. To address this question, herein we describe a set of methodologies using recombinant subviral particles (RSPs) to determine the utilization of HS by clinical strains of DV serotype 1 (DV1). RSPs of clinically isolated strains with low cell culture passage histories were used to study HS interaction. Biochemically characterized RSPs showed dose-dependent binding to immobilized heparin, which could be competed by heparin and HS but not structurally related glycosaminoglycans chondroitin sulfate A and hyaluronic acid. The relevance of heparin and HS biochemical interactions was demonstrated by competition of RSP and DV binding to cells with soluble heparin and HS. Our results demonstrate that clinical strains of DV1 can specifically interact with heparin and HS. Together, these data support the possibility that HS on cell surfaces is utilized in the DV-human infection process. National Institutes of Health (U.S.) (R37 GM057073-13) Singapore-MIT Alliance for Research and Technology 2014-10-09T12:53:38Z 2014-10-09T12:53:38Z 2013-05 2013-04 Article http://purl.org/eprint/type/JournalArticle 01681702 http://hdl.handle.net/1721.1/90814 Artpradit, Charlermchai, Luke N. Robinson, Boris K. Gavrilov, Troy T. Rurak, Mathuros Ruchirawat, and Ram Sasisekharan. “Recognition of Heparan Sulfate by Clinical Strains of Dengue Virus Serotype 1 Using Recombinant Subviral Particles.” Virus Research 176, no. 1–2 (September 2013): 69–77. https://orcid.org/0000-0002-2085-7840 en_US http://dx.doi.org/10.1016/j.virusres.2013.04.017 Virus Research Creative Commons Attribution http://creativecommons.org/licenses/by/3.0/ application/pdf Elsevier Elsevier |
| spellingShingle | Artpradit, Charlermchai Gavrilov, Boris K. Rurak, Troy T. Ruchirawat, Mathuros Sasisekharan, Ram Robinson, Luke Nathaniel Recognition of heparan sulfate by clinical strains of dengue virus serotype 1 using recombinant subviral particles |
| title | Recognition of heparan sulfate by clinical strains of dengue virus serotype 1 using recombinant subviral particles |
| title_full | Recognition of heparan sulfate by clinical strains of dengue virus serotype 1 using recombinant subviral particles |
| title_fullStr | Recognition of heparan sulfate by clinical strains of dengue virus serotype 1 using recombinant subviral particles |
| title_full_unstemmed | Recognition of heparan sulfate by clinical strains of dengue virus serotype 1 using recombinant subviral particles |
| title_short | Recognition of heparan sulfate by clinical strains of dengue virus serotype 1 using recombinant subviral particles |
| title_sort | recognition of heparan sulfate by clinical strains of dengue virus serotype 1 using recombinant subviral particles |
| url | http://hdl.handle.net/1721.1/90814 https://orcid.org/0000-0002-2085-7840 |
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