Effect of polymer chemistry on globular protein–polymer block copolymer self-assembly
Bioconjugates of the model red fluorescent protein mCherry and synthetic polymer blocks with different hydrogen bonding functionalities show that the chemistry of the polymer block has a large effect on both ordering transitions and the type of nanostructures formed during bioconjugate self-assembly...
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| Format: | Article |
| Language: | en_US |
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Royal Society of Chemistry
2014
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| Online Access: | http://hdl.handle.net/1721.1/90939 https://orcid.org/0000-0003-0589-0965 https://orcid.org/0000-0002-7272-7140 https://orcid.org/0000-0002-7845-6425 https://orcid.org/0000-0001-9264-8610 |
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| author | Chang, Dongsook Tang, Shengchang Olsen, Bradley D. Lam, Christopher Nguyen |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Chang, Dongsook Tang, Shengchang Olsen, Bradley D. Lam, Christopher Nguyen |
| author_sort | Chang, Dongsook |
| collection | MIT |
| description | Bioconjugates of the model red fluorescent protein mCherry and synthetic polymer blocks with different hydrogen bonding functionalities show that the chemistry of the polymer block has a large effect on both ordering transitions and the type of nanostructures formed during bioconjugate self-assembly. The phase behaviours of mCherry-b-poly(hydroxypropyl acrylate) (PHPA) and mCherry-b-poly(oligoethylene glycol acrylate) (POEGA) in concentrated aqueous solution show that changes in polymer chemistry result in increase in the order–disorder transition concentrations (C[subscript ODT]s) by approximately 10–15 wt% compared to a previously studied globular protein–polymer block copolymer, mCherry-b-poly(N-isopropylacrylamide) (PNIPAM). The C[subscript ODT]s are always minimized for symmetric bioconjugates, consistent with the importance of protein–polymer interactions in self-assembly. Both mCherry-b-PHPA and mCherry-b-POEGA also form phases that have not previously been observed in other globular protein–polymer conjugates: mCherry-b-PHPA forms a cubic phase that can be indexed to Ia[bar over 3]d and mCherry-b-POEGA displays coexistence of lamellae and a cubic Ia[bar over 3]d structure over a narrow range of concentration and temperature. Several common behaviours are also revealed by comparison of different polymer blocks. With increasing concentration and temperature, ordered phases always appear in the order lamellar, cubic/PL, and hexagonal, although not all phases are observed in all materials. High concentration solutions (near 80 wt%) also undergo a re-entrant order–disorder transition to form nematic liquid crystalline phases, regardless of the polymer block chemistry. |
| first_indexed | 2024-09-23T08:48:15Z |
| format | Article |
| id | mit-1721.1/90939 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T08:48:15Z |
| publishDate | 2014 |
| publisher | Royal Society of Chemistry |
| record_format | dspace |
| spelling | mit-1721.1/909392022-09-23T14:38:58Z Effect of polymer chemistry on globular protein–polymer block copolymer self-assembly Chang, Dongsook Tang, Shengchang Olsen, Bradley D. Lam, Christopher Nguyen Massachusetts Institute of Technology. Department of Chemical Engineering Chang, Dongsook Lam, Christopher Nguyen Tang, Shengchang Olsen, Bradley D. Bioconjugates of the model red fluorescent protein mCherry and synthetic polymer blocks with different hydrogen bonding functionalities show that the chemistry of the polymer block has a large effect on both ordering transitions and the type of nanostructures formed during bioconjugate self-assembly. The phase behaviours of mCherry-b-poly(hydroxypropyl acrylate) (PHPA) and mCherry-b-poly(oligoethylene glycol acrylate) (POEGA) in concentrated aqueous solution show that changes in polymer chemistry result in increase in the order–disorder transition concentrations (C[subscript ODT]s) by approximately 10–15 wt% compared to a previously studied globular protein–polymer block copolymer, mCherry-b-poly(N-isopropylacrylamide) (PNIPAM). The C[subscript ODT]s are always minimized for symmetric bioconjugates, consistent with the importance of protein–polymer interactions in self-assembly. Both mCherry-b-PHPA and mCherry-b-POEGA also form phases that have not previously been observed in other globular protein–polymer conjugates: mCherry-b-PHPA forms a cubic phase that can be indexed to Ia[bar over 3]d and mCherry-b-POEGA displays coexistence of lamellae and a cubic Ia[bar over 3]d structure over a narrow range of concentration and temperature. Several common behaviours are also revealed by comparison of different polymer blocks. With increasing concentration and temperature, ordered phases always appear in the order lamellar, cubic/PL, and hexagonal, although not all phases are observed in all materials. High concentration solutions (near 80 wt%) also undergo a re-entrant order–disorder transition to form nematic liquid crystalline phases, regardless of the polymer block chemistry. United States. Air Force Office of Scientific Research (Award FA9550-12-0259) United States. Dept. of Energy. Office of Basic Energy Sciences (Award DE-SC0007106) 2014-10-15T13:27:10Z 2014-10-15T13:27:10Z 2014-06 2014-03 Article http://purl.org/eprint/type/JournalArticle 1759-9954 1759-9962 http://hdl.handle.net/1721.1/90939 Chang, Dongsook, Christopher N. Lam, Shengchang Tang, and Bradley D. Olsen. “Effect of Polymer Chemistry on Globular Protein–polymer Block Copolymer Self-Assembly.” Polym. Chem. 5, no. 17 (2014): 4884–4895. © Royal Society of Chemistry https://orcid.org/0000-0003-0589-0965 https://orcid.org/0000-0002-7272-7140 https://orcid.org/0000-0002-7845-6425 https://orcid.org/0000-0001-9264-8610 en_US http://dx.doi.org/10.1039/c4py00448e Polymer Chemistry Creative Commons Attribution-Noncommercial http://creativecommons.org/licenses/by-nc/3.0/ application/pdf Royal Society of Chemistry RSC |
| spellingShingle | Chang, Dongsook Tang, Shengchang Olsen, Bradley D. Lam, Christopher Nguyen Effect of polymer chemistry on globular protein–polymer block copolymer self-assembly |
| title | Effect of polymer chemistry on globular protein–polymer block copolymer self-assembly |
| title_full | Effect of polymer chemistry on globular protein–polymer block copolymer self-assembly |
| title_fullStr | Effect of polymer chemistry on globular protein–polymer block copolymer self-assembly |
| title_full_unstemmed | Effect of polymer chemistry on globular protein–polymer block copolymer self-assembly |
| title_short | Effect of polymer chemistry on globular protein–polymer block copolymer self-assembly |
| title_sort | effect of polymer chemistry on globular protein polymer block copolymer self assembly |
| url | http://hdl.handle.net/1721.1/90939 https://orcid.org/0000-0003-0589-0965 https://orcid.org/0000-0002-7272-7140 https://orcid.org/0000-0002-7845-6425 https://orcid.org/0000-0001-9264-8610 |
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