In Vivo Volume and Hemoglobin Dynamics of Human Red Blood Cells
Human red blood cells (RBCs) lose ~30% of their volume and ~20% of their hemoglobin (Hb) content during their ~100-day lifespan in the bloodstream. These observations are well-documented, but the mechanisms for these volume and hemoglobin loss events are not clear. RBCs shed hemoglobin-containing ve...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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Public Library of Science
2014
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| Online Access: | http://hdl.handle.net/1721.1/90976 https://orcid.org/0000-0001-5223-9433 |
| _version_ | 1826190353885036544 |
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| author | Malka, Roy Higgins, John M. Manalis, Scott R Delgado, Francisco Feijo |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Malka, Roy Higgins, John M. Manalis, Scott R Delgado, Francisco Feijo |
| author_sort | Malka, Roy |
| collection | MIT |
| description | Human red blood cells (RBCs) lose ~30% of their volume and ~20% of their hemoglobin (Hb) content during their ~100-day lifespan in the bloodstream. These observations are well-documented, but the mechanisms for these volume and hemoglobin loss events are not clear. RBCs shed hemoglobin-containing vesicles during their life in the circulation, and this process is thought to dominate the changes in the RBC physical characteristics occurring during maturation. We combine theory with single-cell measurements to investigate the impact of vesiculation on the reduction in volume, Hb mass, and membrane. We show that vesicle shedding alone is sufficient to explain membrane losses but not volume or Hb losses. We use dry mass measurements of human RBCs to validate the models and to propose that additional unknown mechanisms control volume and Hb reduction and are responsible for ~90% of the observed reduction. RBC population characteristics are used in the clinic to monitor and diagnose a wide range of conditions including malnutrition, inflammation, and cancer. Quantitative characterization of cellular maturation processes may help in the early detection of clinical conditions where maturation patterns are altered. |
| first_indexed | 2024-09-23T08:39:00Z |
| format | Article |
| id | mit-1721.1/90976 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T08:39:00Z |
| publishDate | 2014 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/909762022-09-23T13:32:55Z In Vivo Volume and Hemoglobin Dynamics of Human Red Blood Cells Malka, Roy Higgins, John M. Manalis, Scott R Delgado, Francisco Feijo Massachusetts Institute of Technology. Department of Biological Engineering Koch Institute for Integrative Cancer Research at MIT Feijo Delgado, Francisco Feijo Manalis, Scott R. Human red blood cells (RBCs) lose ~30% of their volume and ~20% of their hemoglobin (Hb) content during their ~100-day lifespan in the bloodstream. These observations are well-documented, but the mechanisms for these volume and hemoglobin loss events are not clear. RBCs shed hemoglobin-containing vesicles during their life in the circulation, and this process is thought to dominate the changes in the RBC physical characteristics occurring during maturation. We combine theory with single-cell measurements to investigate the impact of vesiculation on the reduction in volume, Hb mass, and membrane. We show that vesicle shedding alone is sufficient to explain membrane losses but not volume or Hb losses. We use dry mass measurements of human RBCs to validate the models and to propose that additional unknown mechanisms control volume and Hb reduction and are responsible for ~90% of the observed reduction. RBC population characteristics are used in the clinic to monitor and diagnose a wide range of conditions including malnutrition, inflammation, and cancer. Quantitative characterization of cellular maturation processes may help in the early detection of clinical conditions where maturation patterns are altered. United States. Army Research Office (Institute for Collaborative Biotechnologies Contract W911NF-09-D-0001) National Institutes of Health (U.S.) (Director's New Innovator Award) National Institutes of Health (U.S.) (Grant 1DP2DK098087) 2014-10-20T12:39:11Z 2014-10-20T12:39:11Z 2014-10 2014-05 Article http://purl.org/eprint/type/JournalArticle 1553-7358 1553-734X http://hdl.handle.net/1721.1/90976 Malka, Roy, Francisco Feijo Delgado, Scott R. Manalis, and John M. Higgins. “In Vivo Volume and Hemoglobin Dynamics of Human Red Blood Cells.” Edited by Daniel A. Beard. PLoS Comput Biol 10, no. 10 (October 9, 2014): e1003839. https://orcid.org/0000-0001-5223-9433 en_US http://dx.doi.org/10.1371/journal.pcbi.1003839 PLoS Computational Biology Creative Commons Attribution http://creativecommons.org/licenses/by/4.0/ application/pdf Public Library of Science Public Library of Science |
| spellingShingle | Malka, Roy Higgins, John M. Manalis, Scott R Delgado, Francisco Feijo In Vivo Volume and Hemoglobin Dynamics of Human Red Blood Cells |
| title | In Vivo Volume and Hemoglobin Dynamics of Human Red Blood Cells |
| title_full | In Vivo Volume and Hemoglobin Dynamics of Human Red Blood Cells |
| title_fullStr | In Vivo Volume and Hemoglobin Dynamics of Human Red Blood Cells |
| title_full_unstemmed | In Vivo Volume and Hemoglobin Dynamics of Human Red Blood Cells |
| title_short | In Vivo Volume and Hemoglobin Dynamics of Human Red Blood Cells |
| title_sort | in vivo volume and hemoglobin dynamics of human red blood cells |
| url | http://hdl.handle.net/1721.1/90976 https://orcid.org/0000-0001-5223-9433 |
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