RecA Protein Plays a Role in the Chemotactic Response and Chemoreceptor Clustering of Salmonella enterica
The RecA protein is the main bacterial recombinase and the activator of the SOS system. In Escherichia coli and Salmonella enterica sv. Typhimurium, RecA is also essential for swarming, a flagellar-driven surface translocation mechanism widespread among bacteria. In this work, the direct interaction...
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| Format: | Article |
| Language: | en_US |
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Public Library of Science
2014
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| Online Access: | http://hdl.handle.net/1721.1/90987 https://orcid.org/0000-0002-4937-7912 https://orcid.org/0000-0002-3199-0508 |
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| author | Mayola, Albert Irazoki, Oihane Martinez, Ignacio A. Petrov, Dmitri Menolascina, Filippo Stocker, Roman Reyes-Darias, Jose A. Krell, Tino Barbe, Jordi Campoy, Susana |
| author2 | Massachusetts Institute of Technology. Department of Civil and Environmental Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Civil and Environmental Engineering Mayola, Albert Irazoki, Oihane Martinez, Ignacio A. Petrov, Dmitri Menolascina, Filippo Stocker, Roman Reyes-Darias, Jose A. Krell, Tino Barbe, Jordi Campoy, Susana |
| author_sort | Mayola, Albert |
| collection | MIT |
| description | The RecA protein is the main bacterial recombinase and the activator of the SOS system. In Escherichia coli and Salmonella enterica sv. Typhimurium, RecA is also essential for swarming, a flagellar-driven surface translocation mechanism widespread among bacteria. In this work, the direct interaction between RecA and the CheW coupling protein was confirmed, and the motility and chemotactic phenotype of a S. Typhimurium ΔrecA mutant was characterized through microfluidics, optical trapping, and quantitative capillary assays. The results demonstrate the tight association of RecA with the chemotaxis pathway and also its involvement in polar chemoreceptor cluster formation. RecA is therefore necessary for standard flagellar rotation switching, implying its essential role not only in swarming motility but also in the normal chemotactic response of S. Typhimurium. |
| first_indexed | 2024-09-23T16:48:58Z |
| format | Article |
| id | mit-1721.1/90987 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T16:48:58Z |
| publishDate | 2014 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/909872022-09-29T21:41:41Z RecA Protein Plays a Role in the Chemotactic Response and Chemoreceptor Clustering of Salmonella enterica Mayola, Albert Irazoki, Oihane Martinez, Ignacio A. Petrov, Dmitri Menolascina, Filippo Stocker, Roman Reyes-Darias, Jose A. Krell, Tino Barbe, Jordi Campoy, Susana Massachusetts Institute of Technology. Department of Civil and Environmental Engineering Menolascina, Filippo Stocker, Roman The RecA protein is the main bacterial recombinase and the activator of the SOS system. In Escherichia coli and Salmonella enterica sv. Typhimurium, RecA is also essential for swarming, a flagellar-driven surface translocation mechanism widespread among bacteria. In this work, the direct interaction between RecA and the CheW coupling protein was confirmed, and the motility and chemotactic phenotype of a S. Typhimurium ΔrecA mutant was characterized through microfluidics, optical trapping, and quantitative capillary assays. The results demonstrate the tight association of RecA with the chemotaxis pathway and also its involvement in polar chemoreceptor cluster formation. RecA is therefore necessary for standard flagellar rotation switching, implying its essential role not only in swarming motility but also in the normal chemotactic response of S. Typhimurium. National Institutes of Health (U.S.) (Grant 1R01GM100473) 2014-10-20T14:50:02Z 2014-10-20T14:50:02Z 2014-08 2014-07 Article http://purl.org/eprint/type/JournalArticle 1932-6203 http://hdl.handle.net/1721.1/90987 Mayola, Albert, Oihane Irazoki, Ignacio A. Martinez, Dmitri Petrov, Filippo Menolascina, Roman Stocker, Jose A. Reyes-Darias, Tino Krell, Jordi Barbe, and Susana Campoy. “RecA Protein Plays a Role in the Chemotactic Response and Chemoreceptor Clustering of Salmonella Enterica.” Edited by Axel Cloeckaert. PLoS ONE 9, no. 8 (August 22, 2014): e105578. https://orcid.org/0000-0002-4937-7912 https://orcid.org/0000-0002-3199-0508 en_US http://dx.doi.org/10.1371/journal.pone.0105578 PLoS ONE Creative Commons Attribution http://creativecommons.org/licenses/by/4.0/ application/pdf Public Library of Science Public Library of Science |
| spellingShingle | Mayola, Albert Irazoki, Oihane Martinez, Ignacio A. Petrov, Dmitri Menolascina, Filippo Stocker, Roman Reyes-Darias, Jose A. Krell, Tino Barbe, Jordi Campoy, Susana RecA Protein Plays a Role in the Chemotactic Response and Chemoreceptor Clustering of Salmonella enterica |
| title | RecA Protein Plays a Role in the Chemotactic Response and Chemoreceptor Clustering of Salmonella enterica |
| title_full | RecA Protein Plays a Role in the Chemotactic Response and Chemoreceptor Clustering of Salmonella enterica |
| title_fullStr | RecA Protein Plays a Role in the Chemotactic Response and Chemoreceptor Clustering of Salmonella enterica |
| title_full_unstemmed | RecA Protein Plays a Role in the Chemotactic Response and Chemoreceptor Clustering of Salmonella enterica |
| title_short | RecA Protein Plays a Role in the Chemotactic Response and Chemoreceptor Clustering of Salmonella enterica |
| title_sort | reca protein plays a role in the chemotactic response and chemoreceptor clustering of salmonella enterica |
| url | http://hdl.handle.net/1721.1/90987 https://orcid.org/0000-0002-4937-7912 https://orcid.org/0000-0002-3199-0508 |
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