Persistent Cellular Motion Control and Trapping Using Mechanotactic Signaling
Chemotactic signaling and the associated directed cell migration have been extensively studied owing to their importance in emergent processes of cellular aggregation. In contrast, mechanotactic signaling has been relatively overlooked despite its potential for unique ways to artificially signal cel...
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| Format: | Article |
| Language: | en_US |
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Public Library of Science
2014
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| Online Access: | http://hdl.handle.net/1721.1/91005 https://orcid.org/0000-0002-0141-2168 https://orcid.org/0000-0003-1273-9964 |
| _version_ | 1826208120523718656 |
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| author | Zhu, Xiaoying Bouffanais, Roland Yue, Dick K. P. |
| author2 | Massachusetts Institute of Technology. Department of Mechanical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Mechanical Engineering Zhu, Xiaoying Bouffanais, Roland Yue, Dick K. P. |
| author_sort | Zhu, Xiaoying |
| collection | MIT |
| description | Chemotactic signaling and the associated directed cell migration have been extensively studied owing to their importance in emergent processes of cellular aggregation. In contrast, mechanotactic signaling has been relatively overlooked despite its potential for unique ways to artificially signal cells with the aim to effectively gain control over their motile behavior. The possibility of mimicking cellular mechanotactic signals offers a fascinating novel strategy to achieve targeted cell delivery for in vitro tissue growth if proven to be effective with mammalian cells. Using (i) optimal level of extracellular calcium ([Ca2[superscript +] ][subscript ext] = 3 mM) we found, (ii) controllable fluid shear stress of low magnitude (σ < 0.5 Pa), and (iii) the ability to swiftly reverse flow direction (within one second), we are able to successfully signal Dictyostelium discoideum amoebae and trigger migratory responses with heretofore unreported control and precision. Specifically, we are able to systematically determine the mechanical input signal required to achieve any predetermined sequences of steps including straightforward motion, reversal and trapping. The mechanotactic cellular trapping is achieved for the first time and is associated with a stalling frequency of 0.06 ~ 0.1 Hz for a reversing direction mechanostimulus, above which the cells are effectively trapped while maintaining a high level of directional sensing. The value of this frequency is very close to the stalling frequency recently reported for chemotactic cell trapping [Meier B, et al. (2011) Proc Natl Acad Sci USA 108:11417–11422], suggesting that the limiting factor may be the slowness of the internal chemically-based motility apparatus. |
| first_indexed | 2024-09-23T14:00:31Z |
| format | Article |
| id | mit-1721.1/91005 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:00:31Z |
| publishDate | 2014 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/910052022-10-01T18:31:22Z Persistent Cellular Motion Control and Trapping Using Mechanotactic Signaling Zhu, Xiaoying Bouffanais, Roland Yue, Dick K. P. Massachusetts Institute of Technology. Department of Mechanical Engineering Bouffanais, Roland Yue, Dick K. P. Chemotactic signaling and the associated directed cell migration have been extensively studied owing to their importance in emergent processes of cellular aggregation. In contrast, mechanotactic signaling has been relatively overlooked despite its potential for unique ways to artificially signal cells with the aim to effectively gain control over their motile behavior. The possibility of mimicking cellular mechanotactic signals offers a fascinating novel strategy to achieve targeted cell delivery for in vitro tissue growth if proven to be effective with mammalian cells. Using (i) optimal level of extracellular calcium ([Ca2[superscript +] ][subscript ext] = 3 mM) we found, (ii) controllable fluid shear stress of low magnitude (σ < 0.5 Pa), and (iii) the ability to swiftly reverse flow direction (within one second), we are able to successfully signal Dictyostelium discoideum amoebae and trigger migratory responses with heretofore unreported control and precision. Specifically, we are able to systematically determine the mechanical input signal required to achieve any predetermined sequences of steps including straightforward motion, reversal and trapping. The mechanotactic cellular trapping is achieved for the first time and is associated with a stalling frequency of 0.06 ~ 0.1 Hz for a reversing direction mechanostimulus, above which the cells are effectively trapped while maintaining a high level of directional sensing. The value of this frequency is very close to the stalling frequency recently reported for chemotactic cell trapping [Meier B, et al. (2011) Proc Natl Acad Sci USA 108:11417–11422], suggesting that the limiting factor may be the slowness of the internal chemically-based motility apparatus. SUTD-MIT International Design Centre (Grant IDG31400104) 2014-10-20T18:44:48Z 2014-10-20T18:44:48Z 2014-09 2014-05 Article http://purl.org/eprint/type/JournalArticle 1932-6203 http://hdl.handle.net/1721.1/91005 Zhu, Xiaoying, Roland Bouffanais, and Dick K. P. Yue. “Persistent Cellular Motion Control and Trapping Using Mechanotactic Signaling.” Edited by Ming Dao. PLoS ONE 9, no. 9 (September 10, 2014): e105406. https://orcid.org/0000-0002-0141-2168 https://orcid.org/0000-0003-1273-9964 en_US http://dx.doi.org/10.1371/journal.pone.0105406 PLoS ONE Creative Commons Attribution http://creativecommons.org/licenses/by/4.0/ application/pdf Public Library of Science Public Library of Science |
| spellingShingle | Zhu, Xiaoying Bouffanais, Roland Yue, Dick K. P. Persistent Cellular Motion Control and Trapping Using Mechanotactic Signaling |
| title | Persistent Cellular Motion Control and Trapping Using Mechanotactic Signaling |
| title_full | Persistent Cellular Motion Control and Trapping Using Mechanotactic Signaling |
| title_fullStr | Persistent Cellular Motion Control and Trapping Using Mechanotactic Signaling |
| title_full_unstemmed | Persistent Cellular Motion Control and Trapping Using Mechanotactic Signaling |
| title_short | Persistent Cellular Motion Control and Trapping Using Mechanotactic Signaling |
| title_sort | persistent cellular motion control and trapping using mechanotactic signaling |
| url | http://hdl.handle.net/1721.1/91005 https://orcid.org/0000-0002-0141-2168 https://orcid.org/0000-0003-1273-9964 |
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