Single particle tracking reveals spatial and dynamic organization of the Escherichia coli biofilm matrix
Biofilms are communities of surface-adherent bacteria surrounded by secreted polymers known as the extracellular polymeric substance. Biofilms are harmful in many industries, and thus it is of great interest to understand their mechanical properties and structure to determine ways to destabilize the...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | en_US |
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IOP Publishing
2014
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| Online Access: | http://hdl.handle.net/1721.1/91006 https://orcid.org/0000-0001-8260-338X https://orcid.org/0000-0002-8717-7049 |
| _version_ | 1826210730433576960 |
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| author | Birjiniuk, Alona Nance, Elizabeth Hanes, Justin Ribbeck, Katharina Billings, Amanda Nicole Doyle, Patrick S. |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Birjiniuk, Alona Nance, Elizabeth Hanes, Justin Ribbeck, Katharina Billings, Amanda Nicole Doyle, Patrick S. |
| author_sort | Birjiniuk, Alona |
| collection | MIT |
| description | Biofilms are communities of surface-adherent bacteria surrounded by secreted polymers known as the extracellular polymeric substance. Biofilms are harmful in many industries, and thus it is of great interest to understand their mechanical properties and structure to determine ways to destabilize them. By performing single particle tracking with beads of varying surface functionalization it was found that charge interactions play a key role in mediating mobility within biofilms. With a combination of single particle tracking and microrheological concepts, it was found that Escherichia coli biofilms display height dependent charge density that evolves over time. Statistical analyses of bead trajectories and confocal microscopy showed inter-connecting micron scale channels that penetrate throughout the biofilm, which may be important for nutrient transfer through the system. This methodology provides significant insight into a particular biofilm system and can be applied to many others to provide comparisons of biofilm structure. The elucidation of structure provides evidence for the permeability of biofilms to microscale objects, and the ability of a biofilm to mature and change properties over time. |
| first_indexed | 2024-09-23T14:54:49Z |
| format | Article |
| id | mit-1721.1/91006 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:54:49Z |
| publishDate | 2014 |
| publisher | IOP Publishing |
| record_format | dspace |
| spelling | mit-1721.1/910062022-10-01T23:17:02Z Single particle tracking reveals spatial and dynamic organization of the Escherichia coli biofilm matrix Birjiniuk, Alona Nance, Elizabeth Hanes, Justin Ribbeck, Katharina Billings, Amanda Nicole Doyle, Patrick S. Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Chemical Engineering Birjiniuk, Alona Billings, Amanda Nicole Ribbeck, Katharina Doyle, Patrick S. Biofilms are communities of surface-adherent bacteria surrounded by secreted polymers known as the extracellular polymeric substance. Biofilms are harmful in many industries, and thus it is of great interest to understand their mechanical properties and structure to determine ways to destabilize them. By performing single particle tracking with beads of varying surface functionalization it was found that charge interactions play a key role in mediating mobility within biofilms. With a combination of single particle tracking and microrheological concepts, it was found that Escherichia coli biofilms display height dependent charge density that evolves over time. Statistical analyses of bead trajectories and confocal microscopy showed inter-connecting micron scale channels that penetrate throughout the biofilm, which may be important for nutrient transfer through the system. This methodology provides significant insight into a particular biofilm system and can be applied to many others to provide comparisons of biofilm structure. The elucidation of structure provides evidence for the permeability of biofilms to microscale objects, and the ability of a biofilm to mature and change properties over time. National Science Foundation (U.S.) (CBET-1335938) Cystic Fibrosis Foundation (HANES07XX0) Massachusetts Institute of Technology (Charles E. Reed Faculty Initiative Fund) Burroughs Wellcome Fund (Preterm Birth Research Grant) National Institute of Allergy and Infectious Diseases (U.S.) (F30 Fellowship 1F30AI110053-01) National Institute of Allergy and Infectious Diseases (U.S.) (Training Grant in Toxicology 5 T32 ES7020-37) 2014-10-20T19:11:15Z 2014-10-20T19:11:15Z 2014-08 2014-04 Article http://purl.org/eprint/type/JournalArticle 1367-2630 http://hdl.handle.net/1721.1/91006 Birjiniuk, Alona, Nicole Billings, Elizabeth Nance, Justin Hanes, Katharina Ribbeck, and Patrick S Doyle. “ Single Particle Tracking Reveals Spatial and Dynamic Organization of the Escherichia Coli Biofilm Matrix .” New Journal of Physics 16, no. 8 (August 1, 2014): 085014. © 2014 IOP Publishing Ltd and Deutsche Physikalische Gesellschaft https://orcid.org/0000-0001-8260-338X https://orcid.org/0000-0002-8717-7049 en_US http://dx.doi.org/10.1088/1367-2630/16/8/085014 New Journal of Physics Creative Commons Attribution http://creativecommons.org/licenses/by/3.0/ application/pdf IOP Publishing IOP Publishing |
| spellingShingle | Birjiniuk, Alona Nance, Elizabeth Hanes, Justin Ribbeck, Katharina Billings, Amanda Nicole Doyle, Patrick S. Single particle tracking reveals spatial and dynamic organization of the Escherichia coli biofilm matrix |
| title | Single particle tracking reveals spatial and dynamic organization of the Escherichia coli biofilm matrix |
| title_full | Single particle tracking reveals spatial and dynamic organization of the Escherichia coli biofilm matrix |
| title_fullStr | Single particle tracking reveals spatial and dynamic organization of the Escherichia coli biofilm matrix |
| title_full_unstemmed | Single particle tracking reveals spatial and dynamic organization of the Escherichia coli biofilm matrix |
| title_short | Single particle tracking reveals spatial and dynamic organization of the Escherichia coli biofilm matrix |
| title_sort | single particle tracking reveals spatial and dynamic organization of the escherichia coli biofilm matrix |
| url | http://hdl.handle.net/1721.1/91006 https://orcid.org/0000-0001-8260-338X https://orcid.org/0000-0002-8717-7049 |
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