Remotely Activated Protein-Producing Nanoparticles
The development of responsive nanomaterials, nanoscale systems that actively respond to stimuli, is one general goal of nanotechnology. Here we develop nanoparticles that can be controllably triggered to synthesize proteins. The nanoparticles consist of lipid vesicles filled with the cellular machin...
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| Format: | Article |
| Language: | en_US |
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American Chemical Society (ACS)
2014
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| Online Access: | http://hdl.handle.net/1721.1/91138 https://orcid.org/0000-0001-5629-4798 https://orcid.org/0000-0003-4255-0492 |
| _version_ | 1826209423669854208 |
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| author | Schroeder, Avi Goldberg, Michael Solomon Kastrup, Christian Wang, Yingxia Jiang, Shan Joseph, Brian J. Levins, Christopher G. Kannan, Sneha T. Langer, Robert Anderson, Daniel Griffith |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Schroeder, Avi Goldberg, Michael Solomon Kastrup, Christian Wang, Yingxia Jiang, Shan Joseph, Brian J. Levins, Christopher G. Kannan, Sneha T. Langer, Robert Anderson, Daniel Griffith |
| author_sort | Schroeder, Avi |
| collection | MIT |
| description | The development of responsive nanomaterials, nanoscale systems that actively respond to stimuli, is one general goal of nanotechnology. Here we develop nanoparticles that can be controllably triggered to synthesize proteins. The nanoparticles consist of lipid vesicles filled with the cellular machinery responsible for transcription and translation, including amino acids, ribosomes, and DNA caged with a photolabile protecting group. These particles served as nanofactories capable of producing proteins including green fluorescent protein (GFP) and enzymatically active luciferase. In vitro and in vivo, protein synthesis was spatially and temporally controllable, and could be initiated by irradiating micrometer-scale regions on the time scale of milliseconds. The ability to control protein synthesis inside nanomaterials may enable new strategies to facilitate the study of orthogonal proteins in a confined environment and for remotely activated drug delivery. |
| first_indexed | 2024-09-23T14:22:17Z |
| format | Article |
| id | mit-1721.1/91138 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:22:17Z |
| publishDate | 2014 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/911382022-10-01T20:53:01Z Remotely Activated Protein-Producing Nanoparticles Schroeder, Avi Goldberg, Michael Solomon Kastrup, Christian Wang, Yingxia Jiang, Shan Joseph, Brian J. Levins, Christopher G. Kannan, Sneha T. Langer, Robert Anderson, Daniel Griffith Harvard University--MIT Division of Health Sciences and Technology Massachusetts Institute of Technology. Department of Chemical Engineering Massachusetts Institute of Technology. Department of Chemical Engineering Koch Institute for Integrative Cancer Research at MIT Schroeder, Avi Goldberg, Michael Solomon Kastrup, Christian Wang, Yingxia Jiang, Shan Joseph, Brian J. Levins, Christopher G. Kannan, Sneha T. Langer, Robert Anderson, Daniel Griffith The development of responsive nanomaterials, nanoscale systems that actively respond to stimuli, is one general goal of nanotechnology. Here we develop nanoparticles that can be controllably triggered to synthesize proteins. The nanoparticles consist of lipid vesicles filled with the cellular machinery responsible for transcription and translation, including amino acids, ribosomes, and DNA caged with a photolabile protecting group. These particles served as nanofactories capable of producing proteins including green fluorescent protein (GFP) and enzymatically active luciferase. In vitro and in vivo, protein synthesis was spatially and temporally controllable, and could be initiated by irradiating micrometer-scale regions on the time scale of milliseconds. The ability to control protein synthesis inside nanomaterials may enable new strategies to facilitate the study of orthogonal proteins in a confined environment and for remotely activated drug delivery. National Cancer Institute (U.S.) (MIT-Harvard Center for Cancer Nanotechnology Excellence Grant U54 CA151884) Marie D. and Pierre Casimir-Lambert Fund National Cancer Institute (U.S.) (Cancer Center Support (Core) Grant P30-CA14051) National Institutes of Health (U.S.) (Grant EB000244) 2014-10-21T18:51:16Z 2014-10-21T18:51:16Z 2012-03 2012-03 Article http://purl.org/eprint/type/JournalArticle 1530-6984 1530-6992 http://hdl.handle.net/1721.1/91138 Schroeder, Avi, Michael S. Goldberg, Christian Kastrup, Yingxia Wang, Shan Jiang, Brian J. Joseph, Christopher G. Levins, Sneha T. Kannan, Robert Langer, and Daniel G. Anderson. “Remotely Activated Protein-Producing Nanoparticles.” Nano Lett. 12, no. 6 (June 13, 2012): 2685–2689. https://orcid.org/0000-0001-5629-4798 https://orcid.org/0000-0003-4255-0492 en_US http://dx.doi.org/10.1021/nl2036047 Nano Letters Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf American Chemical Society (ACS) PMC |
| spellingShingle | Schroeder, Avi Goldberg, Michael Solomon Kastrup, Christian Wang, Yingxia Jiang, Shan Joseph, Brian J. Levins, Christopher G. Kannan, Sneha T. Langer, Robert Anderson, Daniel Griffith Remotely Activated Protein-Producing Nanoparticles |
| title | Remotely Activated Protein-Producing Nanoparticles |
| title_full | Remotely Activated Protein-Producing Nanoparticles |
| title_fullStr | Remotely Activated Protein-Producing Nanoparticles |
| title_full_unstemmed | Remotely Activated Protein-Producing Nanoparticles |
| title_short | Remotely Activated Protein-Producing Nanoparticles |
| title_sort | remotely activated protein producing nanoparticles |
| url | http://hdl.handle.net/1721.1/91138 https://orcid.org/0000-0001-5629-4798 https://orcid.org/0000-0003-4255-0492 |
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