Combinatorial discovery of polymers resistant to bacterial attachment
Bacterial attachment and subsequent biofilm formation pose key challenges to the optimal performance of medical devices. In this study, we determined the attachment of selected bacterial species to hundreds of polymeric materials in a high-throughput microarray format. Using this method, we identifi...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2014
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| Online Access: | http://hdl.handle.net/1721.1/91141 https://orcid.org/0000-0001-5629-4798 https://orcid.org/0000-0003-4255-0492 |
| _version_ | 1826190130428248064 |
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| author | Hook, Andrew L. Chang, Chien-Yi Yang, Jing Luckett, Jeni Cockayne, Alan Atkinson, Steve Mei, Ying Bayston, Roger Irvine, Derek J. Williams, Paul Davies, Martyn C. Alexander, Morgan R. Anderson, Daniel Griffith Langer, Robert S |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Hook, Andrew L. Chang, Chien-Yi Yang, Jing Luckett, Jeni Cockayne, Alan Atkinson, Steve Mei, Ying Bayston, Roger Irvine, Derek J. Williams, Paul Davies, Martyn C. Alexander, Morgan R. Anderson, Daniel Griffith Langer, Robert S |
| author_sort | Hook, Andrew L. |
| collection | MIT |
| description | Bacterial attachment and subsequent biofilm formation pose key challenges to the optimal performance of medical devices. In this study, we determined the attachment of selected bacterial species to hundreds of polymeric materials in a high-throughput microarray format. Using this method, we identified a group of structurally related materials comprising ester and cyclic hydrocarbon moieties that substantially reduced the attachment of pathogenic bacteria (Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli). Coating silicone with these 'hit' materials achieved up to a 30-fold (96.7%) reduction in the surface area covered by bacteria compared with a commercial silver hydrogel coating in vitro, and the same material coatings were effective at reducing bacterial attachment in vivo in a mouse implant infection model. These polymers represent a class of materials that reduce the attachment of bacteria that could not have been predicted to have this property from the current understanding of bacteria-surface interactions. |
| first_indexed | 2024-09-23T08:35:30Z |
| format | Article |
| id | mit-1721.1/91141 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T08:35:30Z |
| publishDate | 2014 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/911412022-09-30T09:48:51Z Combinatorial discovery of polymers resistant to bacterial attachment Hook, Andrew L. Chang, Chien-Yi Yang, Jing Luckett, Jeni Cockayne, Alan Atkinson, Steve Mei, Ying Bayston, Roger Irvine, Derek J. Williams, Paul Davies, Martyn C. Alexander, Morgan R. Anderson, Daniel Griffith Langer, Robert S Harvard University--MIT Division of Health Sciences and Technology Massachusetts Institute of Technology. Department of Chemical Engineering Koch Institute for Integrative Cancer Research at MIT Mei, Ying Langer, Robert Anderson, Daniel Griffith Bacterial attachment and subsequent biofilm formation pose key challenges to the optimal performance of medical devices. In this study, we determined the attachment of selected bacterial species to hundreds of polymeric materials in a high-throughput microarray format. Using this method, we identified a group of structurally related materials comprising ester and cyclic hydrocarbon moieties that substantially reduced the attachment of pathogenic bacteria (Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli). Coating silicone with these 'hit' materials achieved up to a 30-fold (96.7%) reduction in the surface area covered by bacteria compared with a commercial silver hydrogel coating in vitro, and the same material coatings were effective at reducing bacterial attachment in vivo in a mouse implant infection model. These polymers represent a class of materials that reduce the attachment of bacteria that could not have been predicted to have this property from the current understanding of bacteria-surface interactions. 2014-10-21T19:17:52Z 2014-10-21T19:17:52Z 2012-08 2012-06 Article http://purl.org/eprint/type/JournalArticle 1087-0156 1546-1696 http://hdl.handle.net/1721.1/91141 Hook, Andrew L, Chien-Yi Chang, Jing Yang, Jeni Luckett, Alan Cockayne, Steve Atkinson, Ying Mei, et al. “Combinatorial Discovery of Polymers Resistant to Bacterial Attachment.” Nature Biotechnology 30, no. 9 (August 12, 2012): 868–875. https://orcid.org/0000-0001-5629-4798 https://orcid.org/0000-0003-4255-0492 en_US http://dx.doi.org/10.1038/nbt.2316 Nature Biotechnology Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Nature Publishing Group PMC |
| spellingShingle | Hook, Andrew L. Chang, Chien-Yi Yang, Jing Luckett, Jeni Cockayne, Alan Atkinson, Steve Mei, Ying Bayston, Roger Irvine, Derek J. Williams, Paul Davies, Martyn C. Alexander, Morgan R. Anderson, Daniel Griffith Langer, Robert S Combinatorial discovery of polymers resistant to bacterial attachment |
| title | Combinatorial discovery of polymers resistant to bacterial attachment |
| title_full | Combinatorial discovery of polymers resistant to bacterial attachment |
| title_fullStr | Combinatorial discovery of polymers resistant to bacterial attachment |
| title_full_unstemmed | Combinatorial discovery of polymers resistant to bacterial attachment |
| title_short | Combinatorial discovery of polymers resistant to bacterial attachment |
| title_sort | combinatorial discovery of polymers resistant to bacterial attachment |
| url | http://hdl.handle.net/1721.1/91141 https://orcid.org/0000-0001-5629-4798 https://orcid.org/0000-0003-4255-0492 |
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