A microfluidic “baby machine” for cell synchronization
Common techniques used to synchronize eukaryotic cells in the cell cycle often impose metabolic stress on the cells or physically select for size rather than age. To address these deficiencies, a minimally perturbing method known as the “baby machine” was developed previously. In the technique, susp...
| Main Authors: | , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Royal Society of Chemistry, The
2014
|
| Online Access: | http://hdl.handle.net/1721.1/91265 https://orcid.org/0000-0001-5223-9433 https://orcid.org/0000-0003-3415-3614 |
| _version_ | 1826201610817110016 |
|---|---|
| author | Shaw, Josephine Payer, Kristofor Robert Son, Sungmin Grover, William H. Manalis, Scott R |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Shaw, Josephine Payer, Kristofor Robert Son, Sungmin Grover, William H. Manalis, Scott R |
| author_sort | Shaw, Josephine |
| collection | MIT |
| description | Common techniques used to synchronize eukaryotic cells in the cell cycle often impose metabolic stress on the cells or physically select for size rather than age. To address these deficiencies, a minimally perturbing method known as the “baby machine” was developed previously. In the technique, suspension cells are attached to a membrane, and as the cells divide, the newborn cells are eluted to produce a synchronous population of cells in the G1 phase of the cell cycle. However, the existing “baby machine” is only suitable for cells which can be chemically attached to a surface. Here, we present a microfluidic “baby machine” in which cells are held onto a surface by pressure differences rather than chemical attachment. As a result, our method can in principle be used to synchronize a variety of cell types, including cells which may have weak or unknown surface attachment chemistries. We validate our microfluidic “baby machine” by using it to produce a synchronous population of newborn L1210 mouse lymphocytic leukemia cells in G1 phase. |
| first_indexed | 2024-09-23T11:54:03Z |
| format | Article |
| id | mit-1721.1/91265 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:54:03Z |
| publishDate | 2014 |
| publisher | Royal Society of Chemistry, The |
| record_format | dspace |
| spelling | mit-1721.1/912652022-10-01T06:48:15Z A microfluidic “baby machine” for cell synchronization Shaw, Josephine Payer, Kristofor Robert Son, Sungmin Grover, William H. Manalis, Scott R Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Mechanical Engineering Massachusetts Institute of Technology. Microsystems Technology Laboratories Koch Institute for Integrative Cancer Research at MIT Manalis, Scott R. Shaw, Josephine Payer, Kristofor Robert Manalis, Scott R. Grover, William H. Son, Sungmin Common techniques used to synchronize eukaryotic cells in the cell cycle often impose metabolic stress on the cells or physically select for size rather than age. To address these deficiencies, a minimally perturbing method known as the “baby machine” was developed previously. In the technique, suspension cells are attached to a membrane, and as the cells divide, the newborn cells are eluted to produce a synchronous population of cells in the G1 phase of the cell cycle. However, the existing “baby machine” is only suitable for cells which can be chemically attached to a surface. Here, we present a microfluidic “baby machine” in which cells are held onto a surface by pressure differences rather than chemical attachment. As a result, our method can in principle be used to synchronize a variety of cell types, including cells which may have weak or unknown surface attachment chemistries. We validate our microfluidic “baby machine” by using it to produce a synchronous population of newborn L1210 mouse lymphocytic leukemia cells in G1 phase. National Cancer Institute (U.S.). Physical Sciences-Oncology Center (U54CA143874) National Institute of General Medical Sciences (U.S.) (EUREKA R01GM085457) 2014-11-03T14:59:24Z 2014-11-03T14:59:24Z 2012-04 2012-03 Article http://purl.org/eprint/type/JournalArticle 1473-0197 1473-0189 http://hdl.handle.net/1721.1/91265 Shaw, Josephine, Kristofor Payer, Sungmin Son, William H. Grover, and Scott R. Manalis. “A Microfluidic ‘baby Machine’ for Cell Synchronization.” Lab Chip 12, no. 15 (2012): 2656. https://orcid.org/0000-0001-5223-9433 https://orcid.org/0000-0003-3415-3614 en_US http://dx.doi.org/10.1039/c2lc40277g Lab on a Chip Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Royal Society of Chemistry, The Prof. Manalis via Howard Silver |
| spellingShingle | Shaw, Josephine Payer, Kristofor Robert Son, Sungmin Grover, William H. Manalis, Scott R A microfluidic “baby machine” for cell synchronization |
| title | A microfluidic “baby machine” for cell synchronization |
| title_full | A microfluidic “baby machine” for cell synchronization |
| title_fullStr | A microfluidic “baby machine” for cell synchronization |
| title_full_unstemmed | A microfluidic “baby machine” for cell synchronization |
| title_short | A microfluidic “baby machine” for cell synchronization |
| title_sort | microfluidic baby machine for cell synchronization |
| url | http://hdl.handle.net/1721.1/91265 https://orcid.org/0000-0001-5223-9433 https://orcid.org/0000-0003-3415-3614 |
| work_keys_str_mv | AT shawjosephine amicrofluidicbabymachineforcellsynchronization AT payerkristoforrobert amicrofluidicbabymachineforcellsynchronization AT sonsungmin amicrofluidicbabymachineforcellsynchronization AT groverwilliamh amicrofluidicbabymachineforcellsynchronization AT manalisscottr amicrofluidicbabymachineforcellsynchronization AT shawjosephine microfluidicbabymachineforcellsynchronization AT payerkristoforrobert microfluidicbabymachineforcellsynchronization AT sonsungmin microfluidicbabymachineforcellsynchronization AT groverwilliamh microfluidicbabymachineforcellsynchronization AT manalisscottr microfluidicbabymachineforcellsynchronization |