Direct In Vivo Electrochemical Detection of Haemoglobin in Red Blood Cells
The electrochemical behavior of iron ion in haemoglobin provides insight to the chemical activity in the red blood cell which is important in the field of hematology. Herein, the detection of haemoglobin in human red blood cells on glassy carbon electrode (GC) was demonstrated. Red blood cells or ra...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2014
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| Online Access: | http://hdl.handle.net/1721.1/91269 https://orcid.org/0000-0001-7215-1439 |
| _version_ | 1826201546602315776 |
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| author | Toh, Rou Jun Peng, Weng Kung Han, Jongyoon Pumera, Martin |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Toh, Rou Jun Peng, Weng Kung Han, Jongyoon Pumera, Martin |
| author_sort | Toh, Rou Jun |
| collection | MIT |
| description | The electrochemical behavior of iron ion in haemoglobin provides insight to the chemical activity in the red blood cell which is important in the field of hematology. Herein, the detection of haemoglobin in human red blood cells on glassy carbon electrode (GC) was demonstrated. Red blood cells or raw blood cells was immobilized on a glassy carbon electrode surface with Nafion films employed to sandwich the layer of biological sample firmly on the electrode surface. Cyclic voltammetry (CV) analyses revealed a well-defined reduction peak for haemoglobin at about −0.30 V (vs. Ag/AgCl) at the red blood cell (GC-Nf-RBC-3Nf) and blood (GC-Nf-B-3Nf) film modified GCE in a pH 3.5 phosphate buffer solution. We further demonstrated that the complex biological conditions of a human red blood cell displayed no interference with the detection of haemoglobin. Such findings shall have an implication on the possibilities of studying the electrochemical behaviour of haemoglobin directly from human blood, for various scientific and clinical purposes. |
| first_indexed | 2024-09-23T11:53:08Z |
| format | Article |
| id | mit-1721.1/91269 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:53:08Z |
| publishDate | 2014 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/912692022-10-01T06:42:23Z Direct In Vivo Electrochemical Detection of Haemoglobin in Red Blood Cells Toh, Rou Jun Peng, Weng Kung Han, Jongyoon Pumera, Martin Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Han, Jongyoon The electrochemical behavior of iron ion in haemoglobin provides insight to the chemical activity in the red blood cell which is important in the field of hematology. Herein, the detection of haemoglobin in human red blood cells on glassy carbon electrode (GC) was demonstrated. Red blood cells or raw blood cells was immobilized on a glassy carbon electrode surface with Nafion films employed to sandwich the layer of biological sample firmly on the electrode surface. Cyclic voltammetry (CV) analyses revealed a well-defined reduction peak for haemoglobin at about −0.30 V (vs. Ag/AgCl) at the red blood cell (GC-Nf-RBC-3Nf) and blood (GC-Nf-B-3Nf) film modified GCE in a pH 3.5 phosphate buffer solution. We further demonstrated that the complex biological conditions of a human red blood cell displayed no interference with the detection of haemoglobin. Such findings shall have an implication on the possibilities of studying the electrochemical behaviour of haemoglobin directly from human blood, for various scientific and clinical purposes. Singapore-MIT Alliance for Research and Technology 2014-11-03T15:16:34Z 2014-11-03T15:16:34Z 2014-08 2014-05 Article http://purl.org/eprint/type/JournalArticle 2045-2322 http://hdl.handle.net/1721.1/91269 Toh, Rou Jun, Weng Kung Peng, Jongyoon Han, and Martin Pumera. “Direct In Vivo Electrochemical Detection of Haemoglobin in Red Blood Cells.” Sci. Rep. 4 (August 28, 2014): 6209. https://orcid.org/0000-0001-7215-1439 en_US http://dx.doi.org/10.1038/srep06209 Scientific Reports Creative Commons Attribution http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Nature Publishing Group Nature Publishing Group |
| spellingShingle | Toh, Rou Jun Peng, Weng Kung Han, Jongyoon Pumera, Martin Direct In Vivo Electrochemical Detection of Haemoglobin in Red Blood Cells |
| title | Direct In Vivo Electrochemical Detection of Haemoglobin in Red Blood Cells |
| title_full | Direct In Vivo Electrochemical Detection of Haemoglobin in Red Blood Cells |
| title_fullStr | Direct In Vivo Electrochemical Detection of Haemoglobin in Red Blood Cells |
| title_full_unstemmed | Direct In Vivo Electrochemical Detection of Haemoglobin in Red Blood Cells |
| title_short | Direct In Vivo Electrochemical Detection of Haemoglobin in Red Blood Cells |
| title_sort | direct in vivo electrochemical detection of haemoglobin in red blood cells |
| url | http://hdl.handle.net/1721.1/91269 https://orcid.org/0000-0001-7215-1439 |
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